Distinguishing nuclei-specific benzo[a]pyrene-induced effects from whole-cell alterations in MCF-7 cells using Fourier-transform infrared spectroscopy

Abstract

Exposure to chemicals such as benzo[a]pyrene (B[a]P) can generate intracellular toxic mechanisms. Fourier-transform infrared (FTIR) spectroscopy is a novel approach that allows the non-destructive analysis of underlying chemical bond alterations in patho-physiological processes. This study set out to examine whether B[a]P-induced whole cell alterations could be distinguished from effects on nuclei of exposed cells. Using attenuated total reflection FTIR (ATR-FTIR) spectroscopy, alterations in nuclei isolated from B[a]P-treated MCF-7 cells concentrated either in G0/G1- or S-phase were observed. B[a]P-induced effects in whole-cells included alterations to lipids, DNA and protein spectral regions. Absorbance areas for protein and DNA/RNA regions in B[a]P-treated whole cells differed significantly (P<0.0001) from vehicle controls and these observations correlated with alterations noted in isolated nuclei. Our findings provide evidence that FTIR spectroscopy has the ability to identify specific chemical-induced alterations.

Biochemical alterations in duckweed and algae induced by carrier solvents: Selection of an appropriate solvent in toxicity testing

“…Alterations in DNA/RNA are always associated with bands at 1250 cm −1 and 1080 cm −1 , and these 2 bands are assigned to asymmetric and symmetric stretching vibrations of phosphodiester linkage .Alterations in phosphodiester linkage were detected in the present study for the 4 solvents, even at doses below the OECD recommended limit. Alterations in carbohydrates are mainly reflected on bands of 1000 cm −1 to 1200 cm −1 .…”

4-Nonylphenol effects on rat testis and sertoli cells determined by spectrochemical techniques coupled with chemometric analysis

“…Attenuated total reflection (ATR)-FTIR or Raman spectroscopies have many advantages over traditional molecular biology techniques (e.g., ELISA, Western blotting, RT-PCR), since they are able to analyse samples in a non-destructive and label-free manner (Andrew Chan and Kazarian, 2016;Butler et al, 2016;Paraskevaidi et al, 2017b), with minimal sample preparation (Butler et al, 2016;Obinaju and Martin, 2016), and allowing a simultaneous analysis of a wide range of different biomolecules (Paraskevaidi et al, 2017b). In the past few years, ATR-FTIR and Raman spectroscopy have been extensively applied in toxicology studies, including in vivo (Chen et al, 2015;Li et al, 2015;Obinaju and Martin, 2016) and in vitro tests (Obinaju et al, 2015;Li et al, 2016;Strong et al, 2016). In addition, their potential for disease diagnosis has also been demonstrated in many publications (Gajjar et al, 2012;Owens et al, 2014;Lima et al, 2015;Paraskevaidi et al, 2017b).…”

Iodine excess induces hepatic, renal and pancreatic injury in female mice as determined by attenuated total reflection Fourier‐transform infrared spectrometry

“…The combination of ATR‐FTIR spectroscopy and PCA‐LDA offers the feasibility of capturing biochemical and biophysical properties of tissues and cells (Duan, Li, et al, 2019; Duan, Liu, et al, 2019; Strong et al, 2016). The relative intensity and area of the infrared absorption bands are dependent on the concentration and structure of each absorbing molecule (Duan, Li, et al, 2019; Maitra et al, 2019; Obinaju et al, 2015). Herein, ATR‐FTIR data the disclosed pronounced separation of different levels of EI‐exposed and control mice liver/kidney/pancreas, indicating obvious differences in the biochemical profiles brought about by EI administration.…”