Distinguish cancer cells based on targeting turn-on fluorescence imaging by folate functionalized green emitting carbon dots

Liu, Qiuling; Xu, Shenghao; Niu, Caixia; Li, Minfeng; He, Dacheng; Lu, Zhong‐Lin; Ma, Lin; Na, Na; Huang, Fu Qiang; Jiang, Hua; Ouyang, Jin

doi:10.1016/j.bios.2014.08.052

Cited by 144 publications

(58 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, HepG2 and A549 cells were used to evaluate the targeting effect of the FA-functionalized liposomes, as the two cell lines were reported to be of FR overexpression (positive cell model, FR[+]) and lack FR expression (negative cell model, FR[−]), respectively 25. Cells were grown in Roswell Park Memorial Institute (RPMI)-1640 supplemented with 10% FBS and antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin) and incubated at 37°C in a humidified atmosphere of 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Enzymatic synthesis and in vitro evaluation of folate-functionalized liposomes

Guo¹,

Xu²,

Liu³

et al. 2017

DDDT

View full text Add to dashboard Cite

In this study, folate–poly(ethylene glycol)3400–cholesterol conjugates (FA–PEG–Chol) were enzymatically synthesized in one step and incorporated into liposomes to prepare folate (FA)-functionalized liposomes for targeted drug delivery. The FA-functionalized liposomes loaded with betulinic acid (BA) (FA-L-BA) were prepared by thin lipid film method. The FA-L-BA was characterized by their morphology, particle size, zeta potential, encapsulation efficiency (EE), stability, cell cytotoxicity and cellular uptake. The average size of FA-L-BA was 222±8 nm. The spherical particles exhibited a negative electrical charge of −20.12±1.45 mV and high EE of 91.61%±1.16%. The liposomes were taken up selectively by HepG2 cells. FA-L-BA showed enhanced cytotoxicity (50% inhibitory concentration [IC50] =63.07±2.22 μg/mL) compared to nontargeted control normal liposomes loaded with BA (L-BA; IC50 =93.14±2.19 μg/mL) in HepG2 cells in vitro. In addition, FA-functionalized liposomes loaded with Ir-1 (FA-L-Ir-1) showed significantly higher cellular uptake in HepG2 cells compared to nontargeted control normal liposomes loaded with Ir-1 (L-Ir-1). This novel approach for the liposomes surface modified with FA by a one-step enzymatic amidation was expected to provide potential application as a drug carrier for active targeted delivery to tumor cells.

show abstract

Section: Methodsmentioning

confidence: 99%

Enzymatic synthesis and in vitro evaluation of folate-functionalized liposomes

Guo¹,

Xu²,

Liu³

et al. 2017

DDDT

View full text Add to dashboard Cite

show abstract

“…Since the first microwave CDs synthesis from glucose and poly(ethylene glycol) (PEG-200) [7], several other precursors had been used: glucose/poly(acrylatesodium) [8], carbohydrates/inorganic salts [9], glycerol/phosphate [9], glycerol/4,7,10-trioxa-1,13-tridecanediamine [10], citric acid (CA)/1,2-ethylenediamine (EDA) [11], CA/amines [12], phytic acid/EDA [13], poloxamer [14], triammonium citrate/soluble phosphate [15], chitosan [16] and dopamine [17]. Depending on the synthesis conditions and for the precursors used, CDs with different dimensions and quantum yields can be obtained [7][8][9][10][11][12][13][14][15][16][17]. CDs microwave synthesized from CA and EDA were used for bioimaging [12] and iodide detection [11] with a reported quantum yield (˚) respectively of 30.2 and 43.8%.…”

Section: Introductionmentioning

confidence: 99%

Peroxynitrite and nitric oxide fluorescence sensing by ethylenediamine doped carbon dots

Simões

Silva

Leitão

2015

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…Before the fluorescence imaging experiments, the A549 cells were transferred and seeded on a 24-well plate with clean slides in culture medium incubating for 24 h at 7 The morphology of the as-prepared CDs was identified by transmission electron microscope (TEM) (Fig.1A) and atomic force microscopy (AFM) (Fig. 1B).…”

Section: Intracellular Imaging Of Fe 3+mentioning

confidence: 99%