Distinctive glycolipid patterns in Wilms' tumor and renal cell carcinoma

Sakakibara, Norikazu; Gasa, Shinsei; Kamio, Koichi; Makita, Akira; Nonomura, K.; Terabe, Masaki; Koyanagi, Tomohiko; Hatae, Y; Takeda, Kazuyoshi

doi:10.1016/0304-3835(91)90155-b

Cited by 26 publications

(21 citation statements)

References 14 publications

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“…The increased synthesis of sulfatide(s) was observed in adenocarcinoma tissues of lung [60], stomach [61, 621, colon [63], and renal cells [64,65]. SM2 was found to be synthesized in cell lines derived from human endometrial adenocarcinoma [66].…”

Section: Discussionmentioning

confidence: 99%

Isolation and structural characterization of a mono‐sulfated isoglobotetraosylceramide, the first sulfoglycosphingolipid of the isoglobo‐series, from rat kidney

Tadano-Aritomi¹,

Kasama²,

Handa³

et al. 1992

European Journal of Biochemistry

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A novel sulfoglycosphingolipid based on the isoglobo-series core structure was isolated from rat kidney and purified by column chromatographies with DEAE-Sephadex and silica beads. The structure was characterized by solvolysis, compositional analysis, proton NMR spectroscopy, Fouriertransform infrared spectroscopy, methylation analysis and liquid secondary ion mass spectrometry (LSIMS). The characteristic fragment ions for a sulfate and a sulfated N-acetylhexosamine were observed in LSIMS spectra. The two-dimensional chemical-shift-correlated spectroscopy (COSY) and nuclear Overhauser enhancement spectroscopy experiments evidenced the presence of a 3-0-sulfated N-acetylgalactosamine and a Gala1 -3Gal structure in the molecule. The major ceramide consisted of 4-hydroxysphinganine linked to a CZ4 nonhydroxy fatty acid, deduced from both compositional analysis and LSIMS. From the above results, the following structure was established for this glycolipid: HS03-3GalNAcfll-3Galal-3Gal~l-4Glcfll-1Cer, isoglobotetraosylceramide (iGb4Cer) IV3-sulfate. Rat kidney also contained globotetraosylceramide (Gb,Cer) IV3-sulfate which has a carbohydrate core identical to that from human kidney. The yields of iGb,Cer IV3-sulfate and Gb4Cer IV3-sulfate were 0.27 and 0.07 nmol/g wet tissue, respectively.

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Section: Discussionmentioning

confidence: 99%

Isolation and structural characterization of a mono‐sulfated isoglobotetraosylceramide, the first sulfoglycosphingolipid of the isoglobo‐series, from rat kidney

Tadano-Aritomi¹,

Kasama²,

Handa³

et al. 1992

European Journal of Biochemistry

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“…For human renal cell carcinoma cell lines, marked increases of CST mRNA and CST activity were observed in six cell lines (SMKT-R1, SMKT-R2, SMKT-R3, SMKT-R4, TOS-1, and TOS-2), whereas ACHN cells showed only a slight increase, as compared with normal cells ( 105 ). Wilms' tumor (nephroblastoma) shows no detection of sulfatide ( 106 ). Thus, elevated expression of sulfatide is not necessarily common in all types of cancer.…”

Section: Hemostasis/thrombosismentioning

confidence: 99%

Role of sulfatide in normal and pathological cells and tissues

Takahashi

Suzuki

2012

Journal of Lipid Research

244

231

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“…Since renal cell carcinoma tissue showed significantly elevated cerebroside sulfotransferase activity [21], the transferase activity was assayed in sera from a number of patients with urological and other cancers.…”

Section: Serum Cerebroside Sulfotransferase Level In Normal Subjects mentioning

confidence: 99%

“…tric carcinoma [20] and renal cell carcinoma [21], all of which histologically belong to the adenocarcinoma type. Furthermore, the increment of the sulfolipid contents in those carcinomas was associated with the enhanced activity of glycolipid sulfotransferase in those tissues [20 -221.…”

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confidence: 99%

Presence and characterization of glycolipid sulfotransferase in human cancer serum

Gasa

Časl

Makita

et al. 1990

European Journal of Biochemistry

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Sulfotransferase, which catalyzes sulfation of the carbohydrate of galactosylceramide (GalCer) and is localised in the Golgi membrane of cells, was assayed for activity in human serum. To do this, an organic solvent was added to the incubated reaction mixture containing GalCer as an acceptor and phosphoadenosine p h~s p h o [~ sulfate as a donor of sulfate to dissociate the synthesized sulfolipid from serum protein. This was followed by isolation of the sulfolipid on an anion-exchange column. Through this procedure, human serum was found to contain sulfotransferase activity. The serum enzyme was activated by Mn2+. K, values of the enzyme for GalCer and 'active sulfate' were 4.6 pM and 5.2 pM, respectively. The enzyme activity was assayed in sera of cancer patients. The serum activity (mean 5 SE, 0.27 0.027 pmol . pl-' . h-l) in renal cell carcinoma patients, whose activity has been demonstrated to be elevated, was significantly ( P < 0.005) increased compared to that of the normal control (mean SE, 0.18 f 0.0014 pmol . pl-' . h-') and of other urological tumors examined.Cerebroside sulfotransferase transfers a sulfate group from 3'-phosphoadenosine 5'-phosphosulfate (PAdoPS) to C3 of the galactose moiety of GalCer to form a sulfolipid [l]. The sulfotransferase activity was demonstrated in particulate fractions from brains of rat [2 -41, sheep [5] and mouse [6,7] and in the Golgi apparatus from rat brain [8] and rat kidneys [9 -111. The localization of cerebroside sulfotransferase is similar to that of glycosaminoglycan sulfotransferases [12] and tyrosylprotein sulfotransferase [13], but different from that of sulfotransferases acting on aryl compounds, hydroxysteroids, estrogens, and bile acids [14], all ofwhich are found in cytosol. Glycosyltransferase activity catalyzing glycolipid synthesis is, in general, hardly detectable in body fluids and no glycolipid sulfotransferase activity has been demonstrated in them.The cerebroside sulfotransferase was solubilized from rat brains [3,15, 161 and from rat kidney [I I] and its enzymatic properties characterized. As to substrate specificity, the sulfotransferase catalyzed the sulfation not only of GalCer but also of LacCer [15] and galactosyldiacylglycerol [17]. Recently, Tennekoon et al. [18] highly purified the sulfotransferase from rat kidney; they demonstrated the presence of lipids bound to the enzyme, consisting of cholesterol and phospholipid, which irreversibly affected the enzyme activity. The sulfotransferase activity of partially delipidated microsomes from mouse brain was modulated by the microsomal lipids in an age-dependent manner [7]. These observations suggest that the enzyme activity is modulated by the surrounding lipid.In our previous studies on human cancer tissues, the sulfolipid increased in human lung adenocarcinoma [ 191, gas-Correspondence to S . Gasa, Biochemistry Laboratory, Cancer Institute, Hokkaido University School of Medicine, N15 W7, Sapporo 060, JapanAbbreviations. GalCer, galactosylceramide (cerebroside) ; LacCer, lactosylceramide...

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Distinctive glycolipid patterns in Wilms' tumor and renal cell carcinoma

Cited by 26 publications

References 14 publications

Isolation and structural characterization of a mono‐sulfated isoglobotetraosylceramide, the first sulfoglycosphingolipid of the isoglobo‐series, from rat kidney

Isolation and structural characterization of a mono‐sulfated isoglobotetraosylceramide, the first sulfoglycosphingolipid of the isoglobo‐series, from rat kidney

Role of sulfatide in normal and pathological cells and tissues

Presence and characterization of glycolipid sulfotransferase in human cancer serum

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