Distinct populations of hypothalamic dopaminergic neurons exhibit differential responses to brain‐derived neurotrophic factor (BDNF) and neurotrophin‐3 (NT3)

Loudes, Catherine; Petit, Florence; Kordon, C.; Faivre-Bauman, A.

doi:10.1046/j.1460-9568.1999.00463.x

Cited by 47 publications

(47 citation statements)

References 43 publications

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“…4D), we concluded that they were specific NT-3 protein forms. Others have described a 35kDa NT-3 species in neuronal tissues and peripheral targets (Huff et al 1997;Loudes et al 1999;Reinshagen et al 2002), but this form was not detected in the current study using the NT-3 antibody from Santa Cruz.…”

Section: Nt-3 Protein Expression In Control Animals and Effects Of Decontrasting

confidence: 70%

Regulation of NGF and NT-3 protein expression in peripheral targets by sympathetic input

2007

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Nerve growth factor (NGF) and neurotrophin-3 (NT-3) are target-derived proteins that regulate innervating sympathetic neurons. Here, we used western blot analysis to investigate changes in NGF and NT-3 protein in several peripheral tissues following loss of sympathetic input. Following removal of the superior cervical ganglion (SCG), large molecular weight (MW) NGF species, including proNGF-A, were increased in distal intracranial SCG targets, such as pineal gland and extracerebral blood vessels (bv). Mature NGF was a minor species in these tissues and unchanged following sympathectomy. Large MW NGF species also were increased when sympathectomy was followed by in vivo NGF administration. Mature NT-3, which was abundant in controls, was significantly decreased in these targets following sympathetic denervation. The decrease in mature NT-3 was enhanced following NGF administration. The trigeminal ganglion, which provides sensory input to these targets, showed increased NGF, but decreased NT-3, in these treatments, demonstrating that decreased NT-3 at the targets did not result from enhanced NT-3 uptake. Unlike pineal gland and extracerebral bv, the external carotid artery, an extracranial proximal SCG target, showed no change in NGF following denervation, and mature NT-3 was significantly increased. Following NGF administration, NT-3 was significantly decreased. We provide evidence for sympathetic regulation of NGF and NT-3 in peripheral targets and that elevated NGF can depress NT-3. The differential response in distal and proximal adult targets is consistent with the idea that neurons innervating proximal and distal targets may serve different roles in regulating neurotrophin protein. In addition, we conclude that previous ELISA results showing increased NGF protein following sympathetic denervation may have resulted from increases in large MW species, rather than an increase in mature NGF.

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Section: Nt-3 Protein Expression In Control Animals and Effects Of Decontrasting

confidence: 70%

Regulation of NGF and NT-3 protein expression in peripheral targets by sympathetic input

2007

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Section: Introductionmentioning

confidence: 99%

“…The BDNF mature protein has been detected in perinatal hypothalamic tissue [10], and in arcuate and periventricular nuclei in the newborn [11]. In contrast to BDNF, NT-3 is found mostly as its precursor form in the neonatal arcuate nucleus, the 14-kD mature protein being only present in the periventricular area [11]. TrkB and TrkC, the respective receptors for BDNF and NT-3, are present in most hypothalamic nuclei in the adult [12, 13, 14]; they have also been described in cultured hypothalamic neurons [15], suggesting an autocrine or a paracrine role for BDNF and NT-3 during development.…”

Section: Introductionmentioning

confidence: 99%

“…BDNF and NT-3 enhance in vitro survival of hypothalamic dopaminergic (DA) neurons [13]. More recently, we also demonstrated that distinct populations of hypothalamic DA neurons exhibit differential responses to BDNF and NT-3: NT-3 enhances morphological and biochemical differentiation of cultured arcuate DA neurons, while BDNF has the same properties, but in periventricular DA neurons only [11]. …”

Section: Introductionmentioning

confidence: 99%

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Brain-Derived Neurotrophic Factor But Not Neurotrophin-3 Enhances Differentiation of Somatostatin Neurons in Hypothalamic Cultures

Loudes

Petit²,

Kordon³

et al. 2000

Neuroendocrinology

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The present work investigated whether neurotrophins could differentially affect in vitro growth and maturation of two related subsets of hypothalamic neurons, hypophysiotropic somatostatin (SRIH) neurons projecting from the periventricular area and arcuate SRIH interneurons. For this purpose, the hypothalamus of 17-day-old rat fetuses was sampled and separated into a ventral and a dorsal fragment containing respectively periventricular and arcuate regions. Each fragment was dissociated and seeded separately in defined medium. Brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3), two important members of the neurotrophin family involved in neuronal differentiation and plasticity, were added to the cultures at seeding time. After 6 or 11 days in vitro, neurons were labeled with an anti-SRIH antiserum and submitted to morphometric analysis. In parallel, SRIH mRNA was estimated by semiquantitative reverse-transcriptase-polymerase chain reaction, and neuronal SRIH content, basal and depolarisation-stimulated releases measured by radioimmunoassay. The response of control, non-labeled neurons was estimated by neuronal counts and by assaying glutamic acid decarboxylase, a marker of a large majority of hypothalamic neurons. BDNF markedly increased the size and the branching number of SRIH periventricular cell bodies. Expression of SRIH mRNA, as well as SRIH content and release into the culture medium, were also stimulated by the neurotrophin. Non-SRIH neurons were not affected by the treatment. Under the same conditions, arcuate neurons exhibited a weak, mostly transient response to BDNF. NT-3 was ineffective on either neuronal subset. Immunoneutralization of Trk receptors provided further evidence for BDNF effect specificity. The results indicate that BDNF is a selective activator of the differentiation of hypophysiotropic SRIH neurons in the periventricular area of the hypothalamus.

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“…In BDNFknockout mice, the density of SRIH cells is drastically reduced in hippocampus and cortex (Gro e et al 2005). In hypothalamic cultures, BDNF has been reported to have a stimulatory effect on SRIH content and mRNA expression (Rage et al 1999) and on differentiation (Loudes et al 1999). …”

Section: Introductionmentioning

confidence: 99%

Involvement of brain-derived neurotrophic factor in the regulation of hypothalamic somatostatin in vivo

Givalois¹,

Naert²,

Tapia‐Arancibia³

et al. 2006

Journal of Endocrinology

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Brain-derived neurotrophic factor (BDNF) has been extensively studied in the central nervous system as a survival and differentiation factor and in plasticity processes. In vitro, BDNF has been shown to stimulate cellular differentiation and neurohormones synthesis and release. We demonstrated that BDNF is a potent and specific stimulatory agent of somatostatin (SRIH) synthesis in primary cultures of hypothalamic neurons. However, less information is available about its function on SRIH neurons in vivo. In the present study, we examined the effect of in vivo intracerebroventricular BDNF administration in adult non-anesthetized male rats. Two distinct experimental approaches were used: acute intracerebroventricular injection and long-term (14 days) continuous infusion (Alzet micro-pumps). We demonstrate that single intracerebroventricular BDNF injections (5 µg/rat) induce an early (60 and 180 min) decrease in the SRIH mRNA signal in the hypothalamic periventricular nucleus (PeVN) accompanied by a decrease of the hypothalamic SRIH content. 48 h after the acute injection, SRIH mRNA levels and peptide content strongly and significantly increased. After continuous intracerebroventricular BDNF administration (12 µg/day for 14 days), a significant increase in the SRIH hypothalamic content was observed. Nevertheless, the increase in peptide content was not correlated with a similar increase in the PeVN messenger level. These findings show the involvement of BDNF in the in vivo regulation of somatostatinergic neurons in adult rats, which clearly differs according to the BDNF administration mode.

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Distinct populations of hypothalamic dopaminergic neurons exhibit differential responses to brain‐derived neurotrophic factor (BDNF) and neurotrophin‐3 (NT3)

Cited by 47 publications

References 43 publications

Regulation of NGF and NT-3 protein expression in peripheral targets by sympathetic input

Regulation of NGF and NT-3 protein expression in peripheral targets by sympathetic input

Brain-Derived Neurotrophic Factor But Not Neurotrophin-3 Enhances Differentiation of Somatostatin Neurons in Hypothalamic Cultures

Involvement of brain-derived neurotrophic factor in the regulation of hypothalamic somatostatin in vivo

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