Distinct domains within the NITROGEN LIMITATION ADAPTATION protein mediate its subcellular localization and function in the nitrate-dependent phosphate homeostasis pathway

Hannam, Carol; Gidda, Satinder K.; Humbert, Sabrina; Peng, Mingsheng; Cui, Yuhai; Dyer, John M.; Rothstein, Steven J.; Mullen, Robert T.

doi:10.1139/cjb-2017-0149

Cited by 5 publications

(5 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the results of the split-ubiquitin Y2H assay showed that only MtNLA3.3 and MtNLA1/MtNLA2 can interact with MtPT1, a Pi-starvation-induced PHT1 family member ( Figure 5 ). Combined with the findings of Hannam et al [ 28 ] and our observation of the subcellular localization of MtNLA3 variants, we suggested that the presence of the second and third SPX subdomains was enough for plasma membrane targeting and to interact with PHT1.…”

Section: Discussionsupporting

confidence: 89%

“…A bipartite nuclear localization signal (NLS) at the N terminus was conserved in Arabidopsis, rice and Medicago NLA1 paralogs ( Figure S1 ). Substitution of the positively charged residues in the NLS or deletion of any SPX subdomains in AtNLA1 disrupted the protein functions and nucleoli and nuclear speckle localization but did not affect protein targeting to the plasma membrane [ 28 ]. If the whole SPX domain is deleted, the truncated proteins are unable to associate with the plasma membrane [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Deletion of the SPX domain interferes with the interaction with PHT1s and blocks the protein-trafficking to the plasma membrane. Further dissection of the importance of each sub-domain in protein localization found that the loss of any SPX subdomain affects nuclear speckle localization [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential Responses of Medicago truncatula NLA Homologs to Nutrient Deficiency and Arbuscular Mycorrhizal Symbiosis

Lin,

Yang,

Hsieh

et al. 2023

Plants

View full text Add to dashboard Cite

NITROGEN LIMITATION ADAPTATION (NLA), a plasma-membrane-associated ubiquitin E3 ligase, plays a negative role in the control of the phosphate transporter family 1 (PHT1) members in Arabidopsis and rice. There are three NLA homologs in the Medicago truncatula genome, but it has been unclear whether the function of these homologs is conserved in legumes. Here we investigated the subcellular localization and the responses of MtNLAs to external phosphate and nitrate status. Similar to AtNLA1, MtNLA1/MtNLA2 was localized in the plasma membrane and nucleus. MtNLA3 has three alternative splicing variants, and intriguingly, MtNLA3.1, the dominant variant, was not able to target the plasma membrane, whereas MtNLA3.2 and MtNLA3.3 were capable of associating with the plasma membrane. In contrast with AtNLA1, we found that MtNLAs were not affected or even upregulated by low-phosphate treatment. We also found that MtNLA3 was upregulated by arbuscular mycorrhizal (AM) symbiosis, and overexpressing MtNLA3.1 in Medicago roots resulted in a decrease in the transcription levels of STR, an essential gene for arbuscule development. Taken together, our results highlight the difference between MtNLA homologs and AtNLA1. Further characterization will be required to reveal the regulation of these genes and their roles in the responses to external nutrient status and AM symbiosis.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Differential Responses of Medicago truncatula NLA Homologs to Nutrient Deficiency and Arbuscular Mycorrhizal Symbiosis

Lin,

Yang,

Hsieh

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Under N deficiency, nla mutants display premature senescence indicating hypersensitivity to the stress ( Peng et al, 2007 ). NLA, which is predominantly localized to the plasma membrane, mediates the ubiquitin-dependent degradation of NRT1.7 ( Liu et al, 2017 ; Hannam et al, 2018 ; Figure 2A ). The phloem expressed NRT1.7 is involved in source-to-sink remobilization of nitrate ( Fan et al, 2009 ).…”

Section: The Ups and Nutrient Stressmentioning

confidence: 99%

The Ubiquitin Proteasome System and Nutrient Stress Response

Mackinnon

Stone

2022

Front. Plant Sci.

View full text Add to dashboard Cite

Plants utilize different molecular mechanisms, including the Ubiquitin Proteasome System (UPS) that facilitates changes to the proteome, to mitigate the impact of abiotic stresses on growth and development. The UPS encompasses the ubiquitination of selected substrates followed by the proteasomal degradation of the modified proteins. Ubiquitin ligases, or E3s, are central to the UPS as they govern specificity and facilitate the attachment of one or more ubiquitin molecules to the substrate protein. From recent studies, the UPS has emerged as an important regulator of the uptake and translocation of essential macronutrients and micronutrients. In this review, we discuss select E3s that are involved in regulating nutrient uptake and responses to stress conditions, including limited or excess levels of nitrogen, phosphorus, iron, and copper.

show abstract

“…

and Pi are also important signaling molecules, and their signaling pathways interact at several levels ( Hu et al., 2019 ; Krouk and Kiba, 2020 ; Torres-Rodriguez et al., 2021 ). Accumulating evidence suggests that the P starvation response (PSR) strongly depends on N availability ( Hannam et al., 2018 ; Hu et al., 2019 ; Medici et al., 2019 ; Ueda et al., 2020 ).

inducible transcription factors, such as HRS1, Hox52, and GLK1, affect the PSR and root development, thereby regulating Pi uptake in response to P deficiency ( Medici et al., 2015 ; Li et al., 2022 ; Wei et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions

et al. 2023

View full text Add to dashboard Cite

Nitrogen (N) and phosphorus (P) are essential phytomacronutrients, and deficiencies in these two elements limit growth and yield in apple (Malus domestica Borkh.). The rootstock plays a key role in the nutrient uptake and environmental adaptation of apple. The objective of this study was to investigate the effects of N and/or P deficiency on hydroponically-grown dwarfing rootstock ‘M9-T337’ seedlings, particularly the roots, by performing an integrated physiological, transcriptomics-, and metabolomics-based analyses. Compared to N and P sufficiency, N and/or P deficiency inhibited aboveground growth, increased the partitioning of total N and total P in roots, enhanced the total number of tips, length, volume, and surface area of roots, and improved the root-to-shoot ratio. P and/or N deficiency inhibited NO3 − influx into roots, and H+ pumps played a important role in the response to P and/or N deficiency. Conjoint analysis of differentially expressed genes and differentially accumulated metabolites in roots revealed that N and/or P deficiency altered the biosynthesis of cell wall components such as cellulose, hemicellulose, lignin, and pectin. The expression of MdEXPA4 and MdEXLB1, two cell wall expansin genes, were shown to be induced by N and/or P deficiency. Overexpression of MdEXPA4 enhanced root development and improved tolerance to N and/or P deficiency in transgenic Arabidopsis thaliana plants. In addition, overexpression of MdEXLB1 in transgenic Solanum lycopersicum seedlings increased the root surface area and promoted acquisition of N and P, thereby facilitating plant growth and adaptation to N and/or P deficiency. Collectively, these results provided a reference for improving root architecture in dwarfing rootstock and furthering our understanding of integration between N and P signaling pathways.

show abstract

Distinct domains within the NITROGEN LIMITATION ADAPTATION protein mediate its subcellular localization and function in the nitrate-dependent phosphate homeostasis pathway

Cited by 5 publications

References 65 publications

Differential Responses of Medicago truncatula NLA Homologs to Nutrient Deficiency and Arbuscular Mycorrhizal Symbiosis

Differential Responses of Medicago truncatula NLA Homologs to Nutrient Deficiency and Arbuscular Mycorrhizal Symbiosis

The Ubiquitin Proteasome System and Nutrient Stress Response

Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions

Contact Info

Product

Resources

About