Distinct chemokine and cytokine gene expression pattern of murine dendritic cells and macrophages in response toMycobacterium tuberculosisinfection

Abstract

In this study, the early innate cytokine and chemokine response of murine dendritic cells (DCs) and macrophages to Mycobacterium tuberculosis infection was compared. The findings indicate a dissimilar gene expression pattern between the two cell types. The expression of IL-12 and IL-23, important for promoting Th1 and Th17 cells, respectively, was up-regulated only in DCs. In addition, expression of CCL1 and CCL17, which are important in recruitment of T regulatory cells, was DC-specific, as was the expression of the immunosuppressive cytokine IL-10. Macrophages, in contrast, exhibited enhanced expression for CCL2 and CXCL10, chemokines that recruit cells to sites of inflammation, and for mycobactericidal molecules NO synthase 2 and TNF. Together, the findings suggest that a component of the innate DC response is not only programmed toward Th1 priming but is also for controlling the magnitude of the Th1 response, and part of the macrophage response is intended for recruiting cells to the lung and for mycobactericidal functions.

Bone Marrow–Specific Deficiency of Nuclear Receptor Nur77 Enhances Atherosclerosis

“…43 The enhanced expression of SDF-1␣ is the most robust difference between Nur77 Ϫ/Ϫ and WT BMM. SDF-1␣ is a pleiotropic chemokine, which is expressed in many cell types including monocytes and macrophages, 44 and, in contrast to other chemokines, this factor is not induced in response to inflammatory stimuli (Figure 1). SDF-1␣ displays multiple functions, among which the induction of migration of monocytes, macrophages, and lymphocytes.…”

Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells

“…Furthermore, compared with macrophages, DCs secrete more MCP-1 in response to M.tb infection (Jang et al, 2008). Therefore, the higher production of MCP-1 and RANTES induced in BCGinfected DCs observed in this study should be significant for protection against TB after vaccination with BCG.…”

Regulation network of serum cytokines induced by tuberculosis-specific antigens reveals biomarkers for tuberculosis diagnosis

“…Some of these cytokines have been previously identified in the context of TB. For example, I-309 was shown to be upregulated in DC cells after TB-specific antigen stimulation (Jang et al, 2008;Yu et al, 2012) as were CXCL9 (MIG) (Bai et al, 2011), IL-10 (Hasan et al, 2011), IL-6 (Zhang et al, 1994, IL-7, IL-8 (Nakaya et al, 1995;Fietta et al, 2002;Boggaram et al, 2013;Huang et al, 2014), and G-CSF (Higgins et al, 2008). IL-10 is ananti-inflammatory molecule that mainly plays a negative role in immune regulation (Boussiotis et al, 2000).…”