Distinct B-cell clonal bands in Helicobacter pylori gastritis with lymphoid hyperplasia

Saxena, Anurag; Moshynska, Oksana; Kanthan, Rani; Bhutani, Mohit; Maksymiuk, Andrew W.; Lukie, Bryan E.

doi:10.1002/(sici)1096-9896(200001)190:1<47::aid-path506>3.3.co;2-f

Cited by 21 publications

(39 citation statements)

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“…Saxona et al reported that B-cell monoclonality was common in Hp gastritis with lymphoid hyperplasia. (50%, 10/20) [26]. They also reported none of these patients had developed lymphoma (follow-up 21-44 months) and that B-cell monoclonality was a useful feature in favoring a reactive process.…”

Section: Discussionmentioning

confidence: 94%

“…Ono et al also reported that B-cell monoclonality was detected in 88% of primary gastric lymphomas, whereas monoclonarity was not observed in reactive lymphoreticular hyperplasia, chronic gastritis (CG), or gastric ulcers [22]. Several authors have reported investigations into the incidence of B-cell monoclonality in Hp-associated gastritis [3,11,15,26]. Calvert reported that B-cell monoclonality was detected in 11% (3/28) of Hp-associated gastritis [3].…”

Section: Discussionmentioning

confidence: 99%

“…Although FG is strongly associated with Hp infection [1,4] and is histologically similar to MALT lymphoma, there are few studies investigating the relationship between FG and MALT lymphoma. [3,11,26] Therefore, we addressed this issue by examining the IgH gene rearrangements to clarify whether FG is a precursor of MALT lymphoma.…”

Section: Introductionmentioning

confidence: 99%

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High incidence of B-cell monoclonality in follicular gastritis: a possible association between follicular gastritis and MALT lymphoma

et al. 2002

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Many studies have indicated a close association between gastric mucosa-associated lymphoid tissue (MALT) lymphoma and Helicobacter pylori ( Hp) infection. Follicular gastritis (FG), a rare type of gastritis, is also closely associated with Hp infection and histologically similar to MALT lymphoma. However, there are few studies investigating the relationship between FG and MALT lymphoma. To clarify the issue, we examined the B-cell monoclonality in FG by immunoglobulin heavy chain (IgH) gene rearrangement. Seventy FG patients (all Hp-positive, 23 males and 47 females, median age 33 yr) were investigated; 70 age-and gender-matched non-FG Hp-positive controls and 24 non-FG Hp-negative controls were also examined. Two gastric biopsies, one from the antrum and one from the corpus, were obtained from each patient. DNA was extracted from formalin-fixed, paraffin-embedded biopsy specimens. IgH gene rearrangement was examined by semi-nested polymerase chain reaction (PCR). In the antral mucosa, monoclonal B-cell populations were detected in 19 (32%) of 60 FG, in 6 (10%) of 60 Hp-positive controls, and in none of 20 Hp-negative controls. In the corporal mucosa, monoclonal B-cell populations were detected in 14 (30%) of 47 FG, in 6 (11%) of 54 Hp-positive controls, and in none of 20 Hp-negative controls. The incidence of monoclonal B-cell populations in the FG patients was higher than in both Hp-positive and Hp-negative controls ( P<0.05). The monoclonal B-cell populations disappeared after successful Hp eradication in 8 of 8 FG patients examined. These data suggest that FG may be strongly associated with MALT lymphoma, and that Hp eradication therapy may be indicated in FG.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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High incidence of B-cell monoclonality in follicular gastritis: a possible association between follicular gastritis and MALT lymphoma

et al. 2002

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“…In this regard, others have shown that non-reproducible pseudomonoclonal bands can also be generated in DNA samples obtained from specimens containing abundant lymphocytic aggregates. 19,24 We recommend that for reliable assessment of IgH PCR-based clonality studies using DNA obtained from fixed, paraffin-embedded tissue extracted samples, no less than 50 ng of DNA should be used as the starting quantity of template DNA, of which B cells should account for at least 5 ng (10% of the total cells). We also recommend that multiple (at least two) aliquots of a DNA sample be subjected to IgH PCR analysis, and that specimens that produce pseudomonoclonal bands be categorized as indeterminate.…”

Section: Discussionmentioning

confidence: 99%

PCR Analysis of the Immunoglobulin Heavy Chain Gene in Polyclonal Processes Can Yield Pseudoclonal Bands as an Artifact of Low B Cell Number

Elenitoba-Johnson¹,

Bohling²,

Mitchell³

et al. 2000

The Journal of Molecular Diagnostics

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Polymerase chain reaction (PCR)-based analysis for detecting immunoglobulin heavy chain gene (IgH)rearrangements in lymphoproliferative disorders is well established. The presence of one or two discrete bands is interpreted as a monoclonal proliferation, whereas a smear pattern represents a polyclonal population. Prompted by our observation of discrete bands in histologically reactive processes with a relative paucity of B cells, we sought to determine whether low numbers of B cells in biopsy specimens could artifactually produce pseudomonoclonal bands. We performed IgH PCR analysis on serially diluted DNA samples from 5 B cell non-Hodgkin's lymphomas (B-NHLs), 5 reactive lymph nodes, 5 reactive tonsils and 10 microdissected germinal centers from a lymph node with follicular hyperplasia. We also assessed multiple aliquots of DNA samples from small biopsy specimens of reactive lymphocytic processes from the stomach (5 cases). PCR products were evaluated using high resolution agarose or polyacrylamide gels, and DNA sequencing was performed on IgH PCR products from two reactive germinal centers, which yielded monoclonal bands of identical size. The utility of molecular techniques such as Southern blot hybridization (SBH) analysis and polymerase chain reaction (PCR) in the assessment of clonality in lymphoproliferative disorders is well established. Although SBH is a fairly sensitive and specific method, its utility as a routine diagnostic tool is hampered by its requirement for high quality DNA, its labor-intensive nature, and its consequent long turnaround time. On the other hand, the rapid and less labor-intensive PCR continues to gain popularity as a technique for the assessment of clonality in lymphoproliferative disorders. 1,2 The results obtained with antigen receptor gene PCR correlate well with the results of SBH. [3][4][5] PCR-based assays have additional advantages over SBH analysis. PCR requires smaller quantities of DNA, and high molecular weight DNA is not necessary. Therefore, PCR has been applied to small biopsies and fixed paraffin-embedded tissues, which are generally unsuitable for SBH analysis. 6 PCR is extremely sensitive and can detect 1 positive cell in a background of 10 5 negative cells. Immunoglobulin heavy chain (IgH) PCR is capable of detecting 1 monoclonal B cell in a background of 10 2 to 10 3 polyclonal B cells. 7 The extreme sensitivity of PCR also constitutes a potential source for pitfalls in the interpretation of PCR-based antigen receptor gene rearrangement studies. Clearly, contamination is a frequent concern. Additionally, we have observed discrete bands in samples obtained from small biopsy specimens in which histological and immunophenotypic evaluation revealed a reactive process. We believe that the discrete bands generated in this situation are related to the paucity of B

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“…In other extranodal sites, similar transient B-cell clonal expansions have also been observed, probably due to local antigen challenge. "Clonal" PCR bands have also been reported in 50% of benign gastric lymphoid hyperplasia (LH) [15] and in 41% of benign lymphoepithelial lesions (BLEL) of the salivary glands [16]. Likewise, spontaneous regression also occurs in gastric LH after Helicobacter pylori eradication, and most BLEL do not progress to salivary gland lymphoma.…”

Section: Discussionmentioning

confidence: 99%

Primary B‐cell lymphoma and lymphoma‐like lesions of the uterine cervix

Chan

Chung

et al. 2003

American J Hematol

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Distinct B-cell clonal bands in Helicobacter pylori gastritis with lymphoid hyperplasia

Cited by 21 publications

References 0 publications

High incidence of B-cell monoclonality in follicular gastritis: a possible association between follicular gastritis and MALT lymphoma

High incidence of B-cell monoclonality in follicular gastritis: a possible association between follicular gastritis and MALT lymphoma

PCR Analysis of the Immunoglobulin Heavy Chain Gene in Polyclonal Processes Can Yield Pseudoclonal Bands as an Artifact of Low B Cell Number

Primary B‐cell lymphoma and lymphoma‐like lesions of the uterine cervix

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