Distant somatic hybridization alters the structure of wheat root bacterial microbiota

Abstract

Root microbiota affect the host plant's adaptation to adverse environments. The structure and function of plant root bacterial communities are affected by soil physicochemical properties and plant genotypes. Distant hybridization is a common breeding strategy to introgress the excellent genes of wild relatives into the crop genome. The effect of distant hybridization on the crop root microbiota has not been reported. In this study, the structure and function of root bacterial community of the somatic hybrid wheat introgression lines SR3 and SR4, which have been respectively reported to be tolerant to salt and saline-alkali stress, and their parent wheat cultivar JN177 as well as common wheat cultivar Chinese Spring (CS) were analyzed using 16S rRNA high-throughput sequencing technology. The results showed that distant hybridization altered the structure and function of wheat (Triticum aestivum L.) root bacterial community. Compared with the parent JN177, some bacterial genera related to plant growth and stress resistance showed higher abundance in SR3 and SR4. Intriguingly, compared with parent JN177, the root bacterial communities of SR3 and SR4 are more similar to that of CS. This indicated that the process of distant hybridization may alter key genes regulating wheat root microbiota.

Advance in Cell Cultures, Cell Fusion and Cell Splitting of Medicinal and Edible Plant

“…It was reported that symmetric somatic hybridization induced a whole-genome scale shift of synonymous codon usage bias (SCUB) via whole genomic shock rather than local chromosomal shock. And there is a complicated association between genetic and epigenetic variation like DNA methylation [82].The process of distant somatic hybridization was recently reported to alter key regulating genes and altered the structure and function of the root bacterial community in somatic hybrid wheat introgression lines SR3 and SR4 [83]. Hence, asymmetric somatic hybridization could be an efficient strategy for wheat breeding.…”

Agricultural sciences and the environment: Reviewing recent technologies and innovations to combat the challenges of climate change, environmental protection, and food security

“…Microorganism application in agriculture is an environmentally friendly agricultural practice that can foster sustainable production (Popescu & Popescu, 2022). Yang, Chen, et al (2022) studied the effect of distant somatic hybridization on wheat root microbiota and reported that it may alter the structure of wheat root bacterial microbiota, concluded that root microbiome is an essential community for plant growth and development. Another paper related to the root bacterial community showed that microbial taxa could be integrated into the management strategy of wheat stripe rust .…”