Dissolved carbon dioxide effects on growth, nutrient consumption, penicillin synthesis and morphology in batch cultures of Penicillium chrysogenum

McNeil, Brian; Harvey, Linda M.; El-Sabbagh, Nasser

doi:10.1016/j.enzmictec.2005.10.020

Cited by 22 publications

(21 citation statements)

References 29 publications

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“…Measurements which offer comparative value at the microscopic level include total hyphal length, hyphal growth unit (ratio of total hyphal length to the total branch number [30]) and the number of tips [31][32][33][34]. Macroscopic factors have included discriminators such as projected area, circularity, eccentricity, and fractal dimension [32,[35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis

Barry¹,

Chan²,

Williams³

2009

J Ind Microbiol Biotechnol

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Mycelial morphology is a critically important process property in industrial fermentations of filamentous micro-organisms, as particular phenotypes are associated with maximum productivity. However, the accurate quantification of complex morphologies still represents a significant challenge in elucidating this relationship. A system has been developed for high-resolution characterisation of filamentous fungal growth on a solid substrate, using membrane immobilization and fully-automatic plug-ins developed for the public domain, Java-based, image-processing software, ImageJ. The system has been used to quantify the microscopic development of Aspergillus oryzae on malt agar, by measuring spore projected area and circularity, the total length of a hyphal element, the number of tips per element, and the hyphal growth unit. Two different stages of growth are described, from the swelling of a population of conidiospores up to fully developed, branched hyphae 24 h after inoculation. Spore swelling expressed as an increase in mean equivalent spore diameter was found to be approximately linear with time. Widespread germination of spores was observed by 8 h after inoculation. From approximately 12 h, the number of tips was found to increase exponentially. The specific growth rate of a population of hyphae was calculated as approximately 0.24-0.27 h(-1). A wide variation in growth kinetics was found within the population. The robustness of the image-analysis system was verified by testing the effect of small variations in the input data.

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Section: Introductionmentioning

confidence: 99%

Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis

Barry¹,

Chan²,

Williams³

2009

J Ind Microbiol Biotechnol

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“…Rapid consumption of glucose was observed in subsequent batches of operation after the first batch of operation. Carbon dioxide produced during microbial metabolism could be effectively removed using column reactors for secondary metabolite production [22].…”

Section: Discussionmentioning

confidence: 99%

Continuous Production of Oxytetracycline in Fluidized Bed Bioreactor by Immobilized Cells of Streptomyces varsoviensis MTCC-1537: Effect of Dilution and Glucose Loading Rates

Jetty

Potumarthi

Rao

et al. 2009

Appl Biochem Biotechnol

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Oxytetracycline (OT) production using glutaraldehyde cross-linked calcium alginate immobilized cells of Streptomyces varsoviensis in continuous fluidized bed reactor (FBR) was investigated. Initially, batch experiments were carried in stirred tank reactor (STR) and FBR using calcium alginate immobilized cells. Higher OT production of 0.45 gm/L was achieved by FBR when compared with 0.33 g/L of OT in STR. All subsequent studies were carried out in continuous mode of operation in FBR. During 21 days of operation, effect of glucose concentration and different dilution rates were studied. A maximum of 0.75 g/L OT was achieved in the medium having 10 g/L of glucose concentration. The highest OT concentration of 0.92 g/L and the highest yield of OT with respect to biomass at 0.1713 g/g were obtained at the dilution rate of 0.25 day(-1).

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“…Some authors reported its stimulatory effects on fungal growth (2,46,47) , while other authors (4,48,49) have considered that CO 2 is an inhibitor of fungal growth. The concentration inducing either the inhibition or the stimulation of fungus activity is variable from one author to another.…”

Section: Dissolved Carbon Dioxidementioning

confidence: 97%

Submerged Liquid Culture for Production of Biomass and Spores ofPenicillium

Canteri¹,

Ghoul²

2015

Food Reviews International

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Mold fungi such as Penicillium, as spores or in vegetative states, are widely used in food applications for texturizing, flavoring or to protect foodstuffs. These spores are usually produced on a solid medium in "Roux flasks". However, to overcome the weaknesses of this process, the use of submerged liquid media has gained in importance over the past years. The aim of this paper is to review the recent progress in the field of production, in submerged liquid culture, of biomass and spores of mold fungi. The effect of the principal factors (medium composition, operating conditions, substrate, oxygen transfer, etc.) on the germination, growth and sporulation phases are reported and discussed.

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Dissolved carbon dioxide effects on growth, nutrient consumption, penicillin synthesis and morphology in batch cultures of Penicillium chrysogenum

Cited by 22 publications

References 29 publications

Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis

Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis

Continuous Production of Oxytetracycline in Fluidized Bed Bioreactor by Immobilized Cells of Streptomyces varsoviensis MTCC-1537: Effect of Dilution and Glucose Loading Rates

Submerged Liquid Culture for Production of Biomass and Spores ofPenicillium

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