Continuous Production of Oxytetracycline in Fluidized Bed Bioreactor by Immobilized Cells of Streptomyces varsoviensis MTCC-1537: Effect of Dilution and Glucose Loading Rates

Abstract

Oxytetracycline (OT) production using glutaraldehyde cross-linked calcium alginate immobilized cells of Streptomyces varsoviensis in continuous fluidized bed reactor (FBR) was investigated. Initially, batch experiments were carried in stirred tank reactor (STR) and FBR using calcium alginate immobilized cells. Higher OT production of 0.45 gm/L was achieved by FBR when compared with 0.33 g/L of OT in STR. All subsequent studies were carried out in continuous mode of operation in FBR. During 21 days of operation, effect of glucose concentration and different dilution rates were studied. A maximum of 0.75 g/L OT was achieved in the medium having 10 g/L of glucose concentration. The highest OT concentration of 0.92 g/L and the highest yield of OT with respect to biomass at 0.1713 g/g were obtained at the dilution rate of 0.25 day(-1).

Dairy effluent treatment and lipids production by Chlorella pyrenoidosa and Euglena gracilis: study on open and closed systems

“…In this context, immobilization of algal cells has been proposed to overcome harvest problem as well as retaining high‐value algal biomass for further processing . Immobilization has been a proven technology for microbial confinement for variety of purposes in the area of industrial biotechnology for enzymes and antibiotics production and in environmental biotechnology for wastewater treatment …”

Voltammetric investigation of complex growth media at a bare glassy carbon electrode: A case study of oxytetracycline

“…OTC has also shown promise as a low-dose/long term growth promoter for livestock [11,12]. Production of OTC by Streptomyces rimosus in fermentation vessels in the bioprocess industry has been explored in a wide range of different microbiological growth media for optimal generation of OTC [13][14][15][16][17] which, coupled with its wide-spread use, provides a relevant case study for this work.…”