The role of physical support in secondary metabolite production by Streptomyces species

Lajtai-Szabó, Piroska; Hülber-Beyer, Éva; Nemestóthy, Nándor; Bélafi–Bakó, Katalin

doi:10.1016/j.bej.2022.108495

Cited by 8 publications

(9 citation statements)

References 59 publications

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“…However, the subject of morphology tailoring is still underrepresented and should be investigated by using the novel tools of metabolic engineering and synthetic biology [ 11 , 12 ]. Surprisingly, only a few studies on morphological engineering with actinomycete have been reported so far in the review articles [ 13 , 14 ]. S. rimosus was only once mentioned by Kuhl et al [ 7 ] without any specific data.…”

Section: Introductionmentioning

confidence: 99%

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)

Ścigaczewska,

Boruta,

Bizukojć

2024

Bioprocess Biosyst Eng

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Streptomyces produce a broad spectrum of biologically active molecules such as oxytetracycline and rimocidin, which are widely used in human and animal treatments. microparticle-enhanced cultivation (MPEC) is one of the tools used for Streptomyces bioprocesses intensification by the control of mycelial morphology. In the present work, morphological changes of Streptomyces rimosus caused by the addition of 10 µm talc microparticles in MPEC were correlated with the biosynthetic activity of the microorganism. Comparing the runs with and without microparticles, major morphological changes were observed in MPEC, including the deformation of pellets, variation of their size, appearance of hyphae and clumps as well as the aggregation of mycelial objects. The presence of talc microparticles also influenced the levels of the studied secondary metabolites produced by S. rimosus. Comparing control and MPEC runs, the addition of talc microparticles increased the amounts of oxytetracycline (9-fold), 2-acetyl-2-decarboxamido-oxytetracycline (7-fold), milbemycin A3+4[O] (3-fold) and CE 108 (1.5-fold), while rimocidin (27-ethyl) and milbemycin β11+4[O] production was reduced. In summary, the addition of talc microparticles to S. rimosus cultivations led to the development of smaller morphological forms like hyphae and clumps as well as to the changes in the amounts of secondary metabolites. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)

Ścigaczewska,

Boruta,

Bizukojć

2024

Bioprocess Biosyst Eng

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“…Nevertheless, factors such as support properties, microbial cell surface, and environmental conditions affect the efficiency of the process. 11,12 Concerning the support materials, the critical factors for cell integration include their porosity and capacity to adhere to cells through van der Waals interactions, ionic forces, hydrogen bonding, or other mechanisms. 12,13 The porous matrices have a high permeability that allows diffusion of oxygen and nutrients needed characteristics when into the material are encapsulated microorganisms for water purification, biomolecule production, and ethanol production.…”

Section: Introductionmentioning

confidence: 99%

“…The adsorption (physical or covalent binding) of the microbial cells on supports (as films and membranes) is a technique also used frequently with this goal. Nevertheless, factors such as support properties, microbial cell surface, and environmental conditions affect the efficiency of the process. , …”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, factors such as support properties, microbial cell surface, and environmental conditions affect the efficiency of the process. 11 , 12 …”

Section: Introductionmentioning

confidence: 99%

“…Concerning the support materials, the critical factors for cell integration include their porosity and capacity to adhere to cells through van der Waals interactions, ionic forces, hydrogen bonding, or other mechanisms. , The porous matrices have a high permeability that allows diffusion of oxygen and nutrients needed characteristics when into the material are encapsulated microorganisms for water purification, biomolecule production, and ethanol production . Other applications of these matrices are in tissue engineering, drug delivery systems, wound healing, as well as in the production of probiotic food, biosorption and bioremediation of chemicals, and antimicrobial food packaging .…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and Design of a Synthetic-Living Material Composed of Chitosan, Calendula officinalis Hydroalcoholic Extract, and Yeast with Applications as a Biocatalyst

et al. 2023

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Design and development of materials that couple synthetic and living components allow taking advantage of the complexity of biological systems within a controlled environment. However, their design and fabrication represent a challenge for material scientists since it is necessary to synthesize synthetic materials with highly specialized biocompatible and physicochemical properties. The design of synthetic-living materials (vita materials) requires materials capable of hosting cell ingrowth and maintaining cell viability for extended periods. Vita materials offer various advantages, from simplifying product purification steps to controlling cell metabolic activity and improving the resistance of biological systems to external stress factors, translating into reducing bioprocess costs and diversifying their industrial applications. Here, chitosan sponges, functionalized with Calendula officinalis hydroalcoholic extract, were synthesized using the freeze-drying method; they showed small pore sizes (7.58 μm), high porosity (97.95%), high water absorption (1695%), and thermal stability, which allows the material to withstand sterilization conditions. The sponges allowed integration of 58.34% of viable Saccharomyces cerevisiae cells, and the cell viability was conserved 12 h post-process (57.14%) under storage conditions [refrigerating temperature (4 °C) and without a nutrient supply]. In addition, the synthesized vita materials conserved their biocatalytic activity after 7 days of the integration process, which was evaluated through glucose consumption and ethanol production. The results in this paper describe the synthesis of complex vita materials and demonstrate that biochemically modified chitosan sponges can be used as a platform material to host living and metabolically active yeast with diverse applications as biocatalysts.

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Antifungal Activity Enhancement of Cell-Free Streptomyces griseus Extract Obtained by Fermentation with Magnetic Manganese Ferrite Nanoparticles

Farias-Vazquez,

Ramos-González,

Pacios-Michelena

et al. 2024

Appl Biochem Biotechnol

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The role of physical support in secondary metabolite production by Streptomyces species

Cited by 8 publications

References 59 publications

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)

Synthesis and Design of a Synthetic-Living Material Composed of Chitosan, Calendula officinalis Hydroalcoholic Extract, and Yeast with Applications as a Biocatalyst

Antifungal Activity Enhancement of Cell-Free Streptomyces griseus Extract Obtained by Fermentation with Magnetic Manganese Ferrite Nanoparticles

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