Dissociation studies on the 80 S ribosome from Pisum sativum

Phillips, Marshall; Hersh, Robert T.

doi:10.1016/0014-4827(70)90459-3

Experimental Cell Research

1970

DOI: 10.1016/0014-4827(70)90459-3

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Dissociation studies on the 80 S ribosome from Pisum sativum

Marshall Phillips

Robert T. Hersh

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1971

1976

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Cited by 6 publications

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“…These salt-dissociated subunits were active in protein synthesis. Dickman (3,20,26). But at present there is no evidence that plant ribosomes can be dissociated into subunits which are active in aminoacyl-tRNA binding and in amino acid incorporation into protein.…”

mentioning

confidence: 99%

Dissociation of N₂ Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea

Lin

Key

1971

Plant Physiol.

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The dissociation of N2 gas-induced monomeric ribosomes from the pea root was studied by varying the concentration of KCI (or NILCI) and MgCL2 in the presence of dithiothreitoL These monoribosomes were shown to dissociate completely into subunits at 0.5 M KCI or NH4CI in the presence of 5 mM MgCI2. The 40S subunits were more susceptible to structural change in KC1 than were the 60S subunits. On the other hand, the 60S subunits appeared to be more labile to NH4CI.The activity of the subunits relative to aminoacyl-tRNA binding and peptide bond formation was investigated using subunits derived from 0.5 M KCI (or NH4CI)

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mentioning

confidence: 99%

Dissociation of N₂ Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea

Lin

Key

1971

Plant Physiol.

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Characterization of unfolded and compact ribosomal subunits from plants and their relationship to those of lower and higher animals: Evidence for physicochemical heterogeneity among eucaryotic ribosomes

Cammarano¹,

Pons²,

Romeo³

et al. 1972

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

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Size heterogeneity of the large ribosomal subunits and conservation of the small subunits in eucaryote evolution

Cammarano¹,

Romeo²,

Gentile³

et al. 1972

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

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Dissociation studies on the 80 S ribosome from Pisum sativum

Cited by 6 publications

References 17 publications

Dissociation of N₂ Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea

Dissociation of N₂ Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea

Characterization of unfolded and compact ribosomal subunits from plants and their relationship to those of lower and higher animals: Evidence for physicochemical heterogeneity among eucaryotic ribosomes

Size heterogeneity of the large ribosomal subunits and conservation of the small subunits in eucaryote evolution

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Dissociation studies on the 80 S ribosome from Pisum sativum

Cited by 6 publications

References 17 publications

Dissociation of N2 Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea

Dissociation of N2 Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea

Characterization of unfolded and compact ribosomal subunits from plants and their relationship to those of lower and higher animals: Evidence for physicochemical heterogeneity among eucaryotic ribosomes

Size heterogeneity of the large ribosomal subunits and conservation of the small subunits in eucaryote evolution

Contact Info

Product

Resources

About

Dissociation of N₂ Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea

Dissociation of N₂ Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea