Dissipation and residue determination of penicillin G and its two metabolites in citrus under field conditions by DSPE/UPLC–MS/MS

Zhou, Jie; An, Jiao; Dong, C.Y.; Zhang, Yaohai; Zhao, Qiyang; Cui, Yongliang

doi:10.1002/bmc.4962

Cited by 6 publications

(4 citation statements)

References 27 publications

(29 reference statements)

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“…DP1 ( m / z 353), DP3 ( m / z 309), DP4 ( m / z 335), DP5 ( m / z 335), and DP6 ( m / z 194) were identified as penicilloic acid, penilloic acid, penillic acid, isopenicillic acid, and phenylacetylglycine, respectively. They also were detected under various agricultural conditions and sewage treatment by other researchers [ 17 , 26 , 37 , 38 ]. DP2 ( m / z 309), DP7 ( m / z 160), and DP9 ( m / z 161) were named as 2-(5, 5-dimethylthiazolidin-2-yl)-2-(2-phenylacetamido) acetic acid, 4, 4-dimethyl-7-oxa-3-thia-1-azabicyclo [3.2.0] heptan-6-one, and 5,5-dimethylthiazolidine-4-carboxylic acid, respectively.…”

Section: Resultsmentioning

confidence: 77%

Effects of pH and Metal Ions on the Hydrothermal Treatment of Penicillin: Kinetic, Pathway, and Antibacterial Activity

Zhang

Niu

et al. 2022

IJERPH

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Antibiotic residues lead to the risk of resistance gene enrichment, which is the main reason why penicillin mycelial dreg (PMD) is defined as hazardous waste. Hydrothermal treatment (HT) is an effective method to treat penicillin mycelial dreg, but the degradation mechanism of penicillin is unclear. In the study, we researched the effects of pH (4–10) at 80–100 °C and metal ions (Mn2+, Fe2+, Cu2+, and Zn2+) at several concentrations on the HT of penicillin, identified the degradation products (DPs) under different conditions, and evaluated the antibacterial activity of hydrothermally treated samples. The results show that penicillin degradation kinetics highly consistent with pseudo-first-order model (R2 = 0.9447–0.9999). The degradation rates (k) at pH = 4, 7, and 10 were 0.1603, 0.0039, and 0.0485 min−1, indicating acidic conditions were more conducive to penicillin degradation. Among the four tested metal ions, Zn2+ had the most significant catalytic effect. Adding 5 mg·L−1 Zn2+ caused 100% degradation rate at pH = 7 after HT for 60 min. Six degradation products (DPs) with low mass-to-charge (m/z ≤ 335) were detected under acidic condition. However, only two and three DPs were observed in the samples catalyzed by Zn2+ and alkali, respectively, and penilloic acid (m/z = 309) was the main DPs under these conditions. Furthermore, no antibacterial activity to Bacillus pumilus was detected in the medium with up to 50% addition of the treated samples under acidic condition. Even though acid, alkali, and some metal ions can improve the degradation ability of penicillin, it was found that the most effective way for removing its anti-bacterial activity was under the acidic condition. Therefore, resistance residue indicates the amount of additive in the process of resource utilization, and avoids the enrichment of resistance genes.

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Section: Resultsmentioning

confidence: 77%

Effects of pH and Metal Ions on the Hydrothermal Treatment of Penicillin: Kinetic, Pathway, and Antibacterial Activity

Zhang

Niu

et al. 2022

IJERPH

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“…In recent years, with increasing usage, penicillin G contamination has been widely detected in surface water, groundwater, sewage water, and sometimes in drinking water [1][2][3] . Penicillin G and its intermediates could cause potential secondary water pollution [4,5] . There are various degradation methods for penicillin G, such as physical degradation, chemical degradation, and biodegradation, among which biodegradation has attracted much attention because of its green, environmental, friendly, and efficient advantages [6,7] .…”

Section: Introductionmentioning

confidence: 99%

Investigation of the mechanism of accelerated biodegradation ofParacoccus-KDSPL-02

Wang,

Xu,

Shen

et al. 2024

Preprint

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Paracoccus-KDSPL-02 can accelerate to degrade penicillin G under light remain poorly understood, largely due to the lack of high-throughput genome engineering tools. Firstly, this study sequenced the genome of Paracoccus-KDSPL-02 and mined the genes that might be involved, and in order to understand in detail whether the expression of the mined genes changed during light. Further, for genes with altering transcriptional levels under light, this study obtained PROKKA_01468 which a photoreceptor protein in Paracoccus-KDSPL-02. In the end, for validating the function of PROKKA_01468, this study knocked down the sequence of the PROKKA_01468 by applying gene editing system, and the knockdown strain showed significant change in the rate of degradation of phenylacetic acid, which is the intermediate product of penicillin G degradation, by light compared with darkness, so that the PROKKA_01468 is the most effective photoreceptor protein in Paracoccus-KDSPL-02

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“…However, with increased use, penicillin G contamination has been widely detected in surface water, groundwater, sewage water, and sometimes in drinking water [10,11]. Penicillin G and its intermediates could cause potential secondary water pollution [12,13].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

et al. 2021

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Background The Paracoccus sp. strain isolated from sludge was identified and evaluated for catalytic activity in the degradation of penicillin G. Results High degradation efficiency and synergistic catalytic effects of the whole cell and visible light without additional catalysts were observed. The key factors influencing the degradation and kinetics of penicillin G were investigated. The results showed the phenylacetic acid, which was produced during penicillin G biodegradation, exhibited stronger inhibiting effects on KDSPL-02. However, this effect was reduced by visible light irradiation without any additional photocatalyst; furthermore, the rate of penicillin G biodegradation was accelerated, reaching a 100% rate in 12 h at a penicillin G concentration of 1.2 g/L. Four key intermediates produced during penicillin G degradation were isolated and identified by LC–MS, 1H NMR, and 13C NMR. Enzymes involved in the PAA pathway were proposed from a genomic analysis of KDSPL-02. Conclusions These results provide a new method for bio-degrading of penicillin or other antibiotic pollutants using photoaccelerating biocatalysts with greater efficiency and more environmentally friendly conditions.

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Dissipation and residue determination of penicillin G and its two metabolites in citrus under field conditions by DSPE/UPLC–MS/MS

Cited by 6 publications

References 27 publications

Effects of pH and Metal Ions on the Hydrothermal Treatment of Penicillin: Kinetic, Pathway, and Antibacterial Activity

Effects of pH and Metal Ions on the Hydrothermal Treatment of Penicillin: Kinetic, Pathway, and Antibacterial Activity

Investigation of the mechanism of accelerated biodegradation ofParacoccus-KDSPL-02

Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

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