Dissemination of metallo-β-lactamase in Pseudomonas aeruginosa
 isolates in Egypt: mutation in bla
VIM-4

Hashem, Hany R.; Hanora, Amro; Abdalla, Salah; Shaeky, Alaa; Saad, Alaa

doi:10.1111/apm.12669

Cited by 19 publications

(19 citation statements)

References 23 publications

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“…Also, Rossi Goncalves23 concluded a high rate of MDR (73.9%). However, a lower rate of MDR was reported in other studies conducted in Egypt 24,25. This high rate of resistance among our isolates may be due to the fact that they were from hospital-acquired infections 26…”

Section: Discussioncontrasting

confidence: 56%

Virulence Factors Of Carbapenem-Resistant Pseudomonas aeruginosa In Hospital-Acquired Infections In Mansoura, Egypt

El-Mahdy¹,

El-Kannishy²

2019

IDR

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PurposeThe problem of carbapenem-resistant Pseudomonas aeruginosa in health-care settings is growing worse. This study was conducted to investigate the rate of carbapenemase genes, antibiotic resistance, and virulence factors in carbapenem-resistant P. aeruginosa associated with hospital-acquired infections.Patients and methodsIsolates of P. aeruginosa were collected from patients with hospital-acquired infections at Mansoura University Hospital in Mansoura. Carbapenem susceptibility was done by broth dilution. The presence of carbapenemase genes and quorum-sensing genes was assessed by PCR. Production of protease, pyocyanin, twitching motility, hemolytic activity and biofilm formation was evaluated.ResultsOut of 80 P. aeruginosa isolates, 34 (42.5%) were resistant to carbapenem. Among carbapenem-resistant P. aeruginosa isolates, 21 (61.8%) were carbapenemase producers. The most prevalent gene detected was blaVIM. The frequency of protease, pyocyanin, twitching motility, hemolytic activity and biofilm formation was 76.2%, 58.8%, 83.8%, 93.8% and 77.5%, respectively. Biofilm formation was significantly associated with carbapenem-resistant P. aeruginosa. On the other hand, pyocyanin production was significantly lower in carbapenem-resistant isolates. No correlation existed between carbapenem resistance and any other studied virulence factors or quorum-sensing genes.ConclusionAssociation of carbapenem-resistant P. aeruginosa with other antibiotic resistance or the presence of virulence factors in hospital-acquired infection may represent a warning that enhances the need for a stringent surveillance program.

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Section: Discussioncontrasting

confidence: 56%

Virulence Factors Of Carbapenem-Resistant Pseudomonas aeruginosa In Hospital-Acquired Infections In Mansoura, Egypt

El-Mahdy¹,

El-Kannishy²

2019

IDR

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“…In the 2017 study conducted by Hashem et al in Ismailia, Egypt, only one (4%) isolate of 147 isolates of P. aeruginosa from different sources had bla IMP , but in the current study, the frequency of this gene was higher among burn patients (25%) (25).…”

Section: Discussioncontrasting

confidence: 63%

Survey on Some Carbapenems and Colistin Resistance Genes Among Pseudomonas aeruginosa Isolates from Burn and Cystic Fibrosis Patients, Tehran, Iran

Talebi

Hakemi-Vala

2019

Arch Clin Infect Dis

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Background: Psseudomonas aerugiona is a major cause of morbidity and mortality among hospitalized patients. Objectives: Because of increasing antibiotic resistance, this study investigated the prevalence of blaIMP, blaPER, blaVEB, blaGES, pmrA, pmrB, and mcr-1 genes in P. aeruginosa isolates among burn and CF (Cystic Fibrosis) patients in Tehran. Methods: During 2016-2017, 80 and 41 isolates of P. aeruginosa were collected from burn and CF patients, respectively, in Shahid Motahhari and Mofid Children's hospitals. Based on the CLSI protocol, an antibiotic susceptibility test was performed using the disk diffusion method. Then PCR and further sequencing were used to evaluate the frequency of the above-named genes. Results: In both groups, high rates of resistance to amikacin, cefepime, imipenem, ciprofloxacin, ceftazidime, aztreonam, piperacillin, gentamycin and piperacillin-tazobactam were detected. Colistin was determined to be the best choice for treatment. In burn patients, the highest frequency was detected for the blaVEB-1 (55%) gene and the frequencies of blaPER-1, blaIMP-1, and blaGES-1 were 27.5%, 25%, and 13.75%, respectively. In CF patients, the blaPER-1 gene was more common (12.19%), and the frequency rates of blaVEB-1 and blaIMP-1 were 2.43% each. The blaGES-1 gene was not detected. Despite the fact that all of the isolates in both groups had pmrA and pmrB genes, different mutations were detected by sequencing. The mcr-1 gene was not shown in all isolates. Conclusions: Hopefully, by the low frequency of mcr-1 gene and the rate of mutation in pmrAB genes in this study, the rate of resistance can keep low with caution prescription to polymyxins.

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“…The results shown in Table 1 indicate that in Egypt from 2000 through 2020, resistance to various cephalosporins, such as cefuroxime, cefotaxime, ceftazidime, and cefepime, was higher than that to b-lactam/b-lactamase inhibitor combinations, e.g., amoxicillin-clavulanic acid and piperacillin-tazobactam. A rise in resistance was reported against aminoglycosides; gentamcin/amikacin, ciprofloxacin (a quinolone drug) and carbapenems, i.e., imipenem and meropenem [ 84 , 85 , 86 , 87 , 88 , 89 ]. The increase in antibiotic resistance in these isolates has also been demonstrated by β-lactamase detection.…”

Section: Resultsmentioning

confidence: 99%

Retrospective Analysis on Antimicrobial Resistance Trends and Prevalence of β-lactamases in Escherichia coli and ESKAPE Pathogens Isolated from Arabian Patients during 2000–2020

Nasser

Palwe²,

Bhargava

et al. 2020

Microorganisms

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The production of diverse and extended spectrum β-lactamases among Escherichia coli and ESKAPE pathogens is a growing threat to clinicians and public health. We aim to provide a comprehensive analysis of evolving trends of antimicrobial resistance and β-lactamases among E. coli and ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acine to bacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) in the Arabian region. A systematic review was conducted in Medline PubMed on papers published between January 2000 and February 2020 on countries in the Arab region showing different antibiotic resistance among E. coli and ESKAPE pathogens. A total of n = 119,144 clinical isolates were evaluated for antimicrobial resistance in 19 Arab countries. Among these clinical isolates, 74,039 belonged to E. coli and ESKAPE pathogen. Distribution of antibiotic resistance among E. coli and ESKAPE pathogens indicated that E. coli (n = 32,038) was the predominant pathogen followed by K. pneumoniae (n = 17,128), P. aeruginosa (n = 11,074), methicillin-resistant S. aureus (MRSA, n = 4370), A. baumannii (n = 3485) and Enterobacter spp. (n = 1574). There were no reports demonstrating Enterococcus faecium producing β-lactamase. Analyses revealed 19 out of 22 countries reported occurrence of ESBL (Extended-Spectrum β-Lactamase) producing E. coli and ESKAPE pathogens. The present study showed significantly increased resistance rates to various antimicrobial agents over the last 20 years; for instance, cephalosporin resistance increased from 37 to 89.5%, fluoroquinolones from 46.8 to 70.3%, aminoglycosides from 40.2 to 64.4%, mono-bactams from 30.6 to 73.6% and carbapenems from 30.5 to 64.4%. An average of 36.9% of the total isolates were reported to have ESBL phenotype during 2000 to 2020. Molecular analyses showed that among ESBLs and Class A and Class D β-lactamases, blaCTX-M and blaOXA have higher prevalence rates of 57% and 52.7%, respectively. Among Class B β-lactamases, few incidences of blaVIM 27.7% and blaNDM 26.3% were encountered in the Arab region. Conclusion: This review highlights a significant increase in resistance to various classes of antibiotics, including cephalosporins, β-lactam and β-lactamase inhibitor combinations, carbapenems, aminoglycosides and quinolones among E. coli and ESKAPE pathogens in the Arab region.

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Dissemination of metallo-β-lactamase in Pseudomonas aeruginosa isolates in Egypt: mutation in bla VIM-4

Cited by 19 publications

References 23 publications

<p>Virulence Factors Of Carbapenem-Resistant <em>Pseudomonas aeruginosa</em> In Hospital-Acquired Infections In Mansoura, Egypt</p>

<p>Virulence Factors Of Carbapenem-Resistant <em>Pseudomonas aeruginosa</em> In Hospital-Acquired Infections In Mansoura, Egypt</p>

Survey on Some Carbapenems and Colistin Resistance Genes Among Pseudomonas aeruginosa Isolates from Burn and Cystic Fibrosis Patients, Tehran, Iran

Retrospective Analysis on Antimicrobial Resistance Trends and Prevalence of β-lactamases in Escherichia coli and ESKAPE Pathogens Isolated from Arabian Patients during 2000–2020

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