Dissemination of antibiotic resistance genes and their potential removal by on-farm treatment processes in nine swine feedlots in Shandong Province, China

Ben, Weiwei; Wang, Jian; Pan, Xun; Qiang, Zhimin

doi:10.1016/j.chemosphere.2016.10.013

Cited by 74 publications

(32 citation statements)

References 33 publications

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“…In the past decade, high prevalence rates of sulfonamide resistance have been observed in mainly Gram-negative bacteria isolated from animals and humans all over the world (Wu et al, 2010; Card et al, 2016; Ben et al, 2017). Resistance to sulfonamides occurs principally through the acquisition of the alternative dihydropteroate synthase (DHPS) gene sul , the product of which has a low affinity for sulfonamides (Changkaew et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Diverse Mobile Genetic Elements and Conjugal Transferability of Sulfonamide Resistance Genes (sul1, sul2, and sul3) in Escherichia coli Isolates From Penaeus vannamei and Pork From Large Markets in Zhejiang, China

et al. 2019

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High prevalence rates of sulfonamide resistance genes sul1 , sul2 , and sul3 have been observed in Gram-negative bacteria isolated from humans, domestic animals, and aquaculture species worldwide. We investigated the distribution characteristics, location, conjugative transferability, and genetic environments of sul genes from Escherichia coli isolates collected from Penaeus vannamei and pork samples from three large markets in Zhejiang, China. The prevalence rates of sul genes in sulfonamide-resistant E. coli isolates from P. vannamei and pork samples were 90.0 and 88.6%, respectively, and the prevalence of sul1 and sul2 was significantly higher than that of sul3 ( p < 0.05). Twenty-four representative sul -positive E. coli isolates were analyzed in detail. Southern blot hybridization confirmed that sul genes of E. coli isolates were located on plasmids and/or chromosomes. Transfer of resistance through conjugation was observed in all 18 E. coli isolates harboring sul genes on plasmids. Replicon typing identified seven different incompatibility groups and IncF was the dominant replicon type among sul gene-containing plasmids from both sources. PCR walking analysis indicated that 87.5% (35/40) of sul gene-related fragments carried insertion sequences (ISs) belonging to a variety of families in diverse sites, with IS 26 occurring most frequently. In addition, the sul1 gene was detected mainly in fragments carrying class 1 integrons. Co-location on the same fragment with resistance genes that may contribute to the persistence and dissemination of sul1 and/or sul2 genes. The diversity of mobile genetic elements and resistance genes adjacent to sul3 was much lower than those adjacent to sul1 and sul2 , especially those located in chromosomes, which reduced the transmission potential of the sul3 gene. In conclusion, combined with the results of clonal relatedness analysis by PFGE and MLST of 24 representative E. coli isolates from P. vannamei and pork samples, it showed that a small number of sul genes were vertically transmitted among E. coli from P. vannamei and that horizontal gene transfer was likely the main transmission mechanism of sul genes from both sources. Our results provide important information to better unde...

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Section: Introductionmentioning

confidence: 99%

Diverse Mobile Genetic Elements and Conjugal Transferability of Sulfonamide Resistance Genes (sul1, sul2, and sul3) in Escherichia coli Isolates From Penaeus vannamei and Pork From Large Markets in Zhejiang, China

et al. 2019

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“…For example, heavy metals such as copper and zinc in feed formulations for food animals select for ARGs that co-localize with metal-resistance genes [ 18, 19 ]. Selection pressure afforded by any one of a number of antibiotics and heavy metals (zinc, cadmium, mercury) that contaminate faecal waste or those used in food-producing and hospital environments is sufficient to select for the retention and spread of complex resistance regions [ 20, 21 ]. Understanding of how ARGs assemble on mobile genetic elements, and the extent to which these then traffic through human, food animal and environmental reservoirs, remains limited.…”

Section: Introductionmentioning

confidence: 99%

Porcine commensal Escherichia coli: a reservoir for class 1 integrons associated with IS26

et al. 2017

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Porcine faecal waste is a serious environmental pollutant. Carriage of antimicrobial-resistance genes (ARGs) and virulence-associated genes (VAGs), and the zoonotic potential of commensal Escherichia coli from swine are largely unknown. Furthermore, little is known about the role of commensal E. coli as contributors to the mobilization of ARGs between food animals and the environment. Here, we report whole-genome sequence analysis of 103 class 1 integron-positive E. coli from the faeces of healthy pigs from two commercial production facilities in New South Wales, Australia. Most strains belonged to phylogroups A and B1, and carried VAGs linked with extraintestinal infection in humans. The 103 strains belonged to 37 multilocus sequence types and clonal complex 10 featured prominently. Seventeen ARGs were detected and 97 % (100/103) of strains carried three or more ARGs. Heavy-metal-resistance genes merA, cusA and terA were also common. IS26 was observed in 98 % (101/103) of strains and was often physically associated with structurally diverse class 1 integrons that carried unique genetic features, which may be tracked. This study provides, to our knowledge, the first detailed genomic analysis and point of reference for commensal E. coli of porcine origin in Australia, facilitating tracking of specific lineages and the mobile resistance genes they carry.

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“…Microbial fermentation bed (MFB), septic tank (ST), biogas digester (BD) and natural drying are four commonly used methods to treat waste manure. Ben et al (2017) assessed the effectiveness of these four on-farm waste treatment methods to eliminate seven target ARGs.…”

Section: Antibiotic Resistance Genesmentioning

confidence: 99%

Detection, Occurrence and Fate of Emerging Contaminants in Agricultural Environments

Snow

Cassada

Biswas

et al. 2018

Water Environment Research

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A total of 75 papers published in 2017 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to ecological effects and sampling techniques for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, steroids, antibiotic resistance genes, and engineered nanoparticles agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Fate and Occurrence, Pharmaceutical Metabolites, Anthelmintics, Antibiotic Resistence Genes, and Engineered Nanomaterials.

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Dissemination of antibiotic resistance genes and their potential removal by on-farm treatment processes in nine swine feedlots in Shandong Province, China

Cited by 74 publications

References 33 publications

Diverse Mobile Genetic Elements and Conjugal Transferability of Sulfonamide Resistance Genes (sul1, sul2, and sul3) in Escherichia coli Isolates From Penaeus vannamei and Pork From Large Markets in Zhejiang, China

Diverse Mobile Genetic Elements and Conjugal Transferability of Sulfonamide Resistance Genes (sul1, sul2, and sul3) in Escherichia coli Isolates From Penaeus vannamei and Pork From Large Markets in Zhejiang, China

Porcine commensal Escherichia coli: a reservoir for class 1 integrons associated with IS26

Detection, Occurrence and Fate of Emerging Contaminants in Agricultural Environments

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