Diverse Mobile Genetic Elements and Conjugal Transferability of Sulfonamide Resistance Genes (sul1, sul2, and sul3) in Escherichia coli Isolates From Penaeus vannamei and Pork From Large Markets in Zhejiang, China

Jiang, Han; Cheng, Hui; Liang, Yi; Yu, Shengtao; Yu, Ting; Fang, Jian Jun; C, Zhu

doi:10.3389/fmicb.2019.01787

Cited by 70 publications

(45 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sul 1 gene is a plasmid-borne sulfonamide resistance gene that is linked to the universal and long-known sulfonamide resistance in Gram-negative bacteria [ 31 ]. This gene determined in all selected E. coli isolates.…”

Section: Discussionmentioning

confidence: 99%

Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Raheel¹,

Hassan²,

Salem³

et al. 2020

J Adv Vet Anim Res

View full text Add to dashboard Cite

Objective: The objectives of this study were to determine the biofilm-forming capability and antimicrobial susceptibility of Escherichia coli recovered from bovine endometritis samples. Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated E. coli by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes. Results: A total of 58(48.3%) E. coli isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethoxazole- trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all isolates harbored bla TEM , sul 1, tet A, qnr S, bla CTX-M , and fim H with a percentage of 100%, pap C (40%), and hly A (10%). Fim H was the most prevalent biofilm-associated gene. Conclusion: The present study highlights the high prevalence of multi-drug- resistant E. coli associated with bovine endometritis. The detection of the fim H gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic E. coli .

show abstract

Section: Discussionmentioning

confidence: 99%

Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Raheel¹,

Hassan²,

Salem³

et al. 2020

J Adv Vet Anim Res

View full text Add to dashboard Cite

show abstract

“…Nowday, sulphonamides are rarely used to treat bacterial infections in humans in many regions, but they are still widely used in aquaculture, animal husbandry and veterinary because of the lower price [20]. Massive use plus great potential for penetrate into the environment.…”

Section: Discussionmentioning

confidence: 99%

“…Massive use plus great potential for penetrate into the environment. For these reasons, the concentration of sulfonamides becomes a priority for sewage treatment, rivers and water sources [20]. Sulfonamineresistant genes (sul) have also spread widely for these reason.…”

Section: Discussionmentioning

confidence: 99%

“…After analyzing the genetic environment of sul gene, Jang et al [20] said that compared with the other two genes, the diversity of adjacent genetic transfer elements and the resistance genes of sul3 were lower, and some sul3 even existed on chromosomes, which affected the transmission of sul3. But the studies also showed that [20,47], sul3 is related to type I integron, and can replace sul1 to form atypical type I integron, which emphasize the potential for widespread in the future. This indicates that there is still a certain transmission problem of sul3 at present, but this problem may be solved over time.…”

Section: Discussionmentioning

confidence: 99%

“…Sul3 is a sulfanilamide drug-resistant gene type discovered in 2003 [17]. Since its discovery, sul3 has been successively found in more and more regions, sources and strains [18][19][20][21], among which even have human-originated Escherichia coli [22] Nanning is the capital of Guangxi Province, which is located in the southwest of China. The breeding industry that in Nanning is mainly composed of retail investors.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial and Transconjugants Characteristics of Sul3 Positive Escherichia Coli Isolated from Animals in Nanning, Guangxi Province

Chen

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Sulfonamides is the second most popular antibiotic in many countries, which leads to the widespread emergence of sulfonamides resistance. Sul3 is a late sulfanilamide resistance gene, whose research is relatively little. Result: 46 sul3 positive E. coli strains were separated. A total of 12 ST types were observed, and 1 of those was previously unknown type. The ST350 is the most numerous type. All isolates were multidrug-resistant E. coli, with high antimicrobial rates to penicillin, ceftriaxone sodium, streptomycin, tetracycline, ciprofloxacin, gatifloxacin and chloramphenicol (100%, 73.9%, 82.6%, 100%, 80.4%, 71.7% and 97.8%), and with at least 3 resistance genes in addition to sul3. The plasmids transfered from 3 sul3-positive isolates to C600, the most of which brought 7 antibiotic resistance and increased resistance genes to C600. The transferred sul3 gene and the plasmid that carries it could be stably inherited in the recipient bacteria for at least 20 days. Those plasmids had no effect on the growth of the recipient bacteria, but it would greatly reduce (at least 60 time) the in vitro competitiveness of the strains. Conclusions: In Nanning, these sul3-positive Escherichia coli have strong antimicrobial resistance, and the plasmid carrying sul3 has the ability to transfer multiple resistance genes, so long-term monitoring is necessary. Since the transferred plasmid will greatly reduce the in vitro competitiveness of the strain, we can consider limiting the spread of antimicrobial in this respect.

show abstract

Metabolically Active Prokaryotes and Actively Transcribed Antibiotic Resistance Genes in Sewer Systems: Implications for Public Health and Microbially Induced Corrosion

2021

View full text Add to dashboard Cite

Sewer systems are reservoirs of pathogens and bacteria carrying antibiotic resistance genes (ARGs). However, most recent high-throughput studies rely on DNA-based techniques that cannot provide information on the physiological state of the cells nor expression of ARGs. In this study, wastewater and sewer sediment samples were collected from combined and separate sanitary sewer systems. The metabolically active prokaryote community was evaluated using 16S rRNA amplicon sequencing and actively transcribed ARG abundance was measured using mRNA RT-qPCR. Three ( sul 1, bla TEM , tet (G)) of the eight tested ARGs were quantifiable in select samples. Sewer sediment samples had greater abundance of actively transcribed ARGs compared to wastewater. Microbiome analysis showed the presence of metabolically active family taxa that contain clinically relevant pathogens (Pseudomonadaceae, Enterobacteraceae, Streptococcaceae, Arcobacteraceae, and Clostridiaceae) and corrosion-causing prokaryotes (Desulfobulbaceae and Desulfovibrionaceae) in both matrices. Spirochaetaceae and methanogens were more common in the sediment matrix while Mycobacteraceae were more common in wastewater. The microbiome obtained from 16S rRNA sequencing had a significantly different structure from the 16S rRNA gene microbiome. Overall, this study demonstrates active transcription of ARGs in sewer systems and provides insight into the abundance and physiological state of taxa of interest in the different sewer matrices and sewer types relevant for wastewater-based epidemiology, corrosion, and understanding the hazard posed by different matrices during sewer overflows. Supplementary Information The online version contains supplementary material available at 10.1007/s00248-021-01775-y.

show abstract

Diverse Mobile Genetic Elements and Conjugal Transferability of Sulfonamide Resistance Genes (sul1, sul2, and sul3) in Escherichia coli Isolates From Penaeus vannamei and Pork From Large Markets in Zhejiang, China

Cited by 70 publications

References 40 publications

Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Antimicrobial and Transconjugants Characteristics of Sul3 Positive Escherichia Coli Isolated from Animals in Nanning, Guangxi Province

Metabolically Active Prokaryotes and Actively Transcribed Antibiotic Resistance Genes in Sewer Systems: Implications for Public Health and Microbially Induced Corrosion

Contact Info

Product

Resources

About