Disseminated<i>Salmonella</i>infection

Johari, Muhammad Izzad; Besari, Alwi Muhd; Ghazali, Wan Syamimee Wan; Yusof, Zurkurnai

doi:10.1136/bcr-2018-226337

Cited by 3 publications

(3 citation statements)

References 17 publications

(15 reference statements)

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“…The strain is a common food contaminant, and has been incriminated in several outbreaks in Malaysia in recent years [ 11 ]. Moreover, human infections are being repeatedly reported, with chicken and chicken products being one of the most common sources of infection [ 11 , 27 , 28 ]. Therefore, it has become paramount to investigate and understand the potential role of chicken (meat and meat products) in the dissemination of Salmonella species.…”

Section: Discussionmentioning

confidence: 99%

Virulence Gene Profile, Antimicrobial Resistance and Multilocus Sequence Typing of Salmonella enterica Subsp. enterica Serovar Enteritidis from Chickens and Chicken Products

Zakaria

Hassan

Sharif

et al. 2022

Animals

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This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of Salmonella in the Central Region of Peninsular Malaysia. A total of 45 Salmonella Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples upon cultural isolation and serotyping. Similarly, an antimicrobial susceptibility test based on the Kirby Bauer disk diffusion method as well as antimicrobial resistance AMR genes, virulence determinants and multilocus sequence typing (MLST) typing were conducted after the Whole Genome Sequencing and analysis of the isolates. The results indicate that sequence types ST1925 (63.7%), and ST11 (26.5%) were the predominant out of the seven sequence types identified (ST292, ST329, ST365, ST423 and ST2132). The phenotypic antimicrobial profile corresponds to the genotypic characterization in that the majority of the isolates that exhibited tetracycline, gentamycin and aminoglycoside resistance; they also possessed the tetC and blaTEM β-Lactam resistance genes. However, isolates from cloacal swabs showed the highest number of resistance genes compared to the chicken products (fresh and ready-to-eat meat) samples. Furthermore, most of the virulence genes were found to cluster in the Salmonella pathogenicity island (SPI). In this study, all the isolates were found to possess SPI-1, which codes for the type III secretion system, which functions as actin-binding proteins (SptP and SopE). The virulence plasmid (VP) genes (spvB, spvC) were present in all genotypes except ST365. The findings of this study, particularly with regard to the molecular subtypes and AMR profiles of the Salmonella Enteritidis serotype shows multidrug-resistance features as well as genetic characteristics indicative of high pathogenicity.

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Section: Discussionmentioning

confidence: 99%

Virulence Gene Profile, Antimicrobial Resistance and Multilocus Sequence Typing of Salmonella enterica Subsp. enterica Serovar Enteritidis from Chickens and Chicken Products

Zakaria

Hassan

Sharif

et al. 2022

Animals

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“…Previous studies have indicated that the human gut microbiota is affected by a wide range of host factors such as diet, genetics, disease, region, and medical conditions (He et al, 2018;Wan et al, 2019). Envfit (see Methods) was conducted to quantitatively evaluate the effect of individual infant factors on the gut microbiota.…”

Section: Multi-factorial Impacts and Disorganized Development Of Gut ...mentioning

confidence: 99%

“…Clinical studies have demonstrated distinct compositions of gut microbiota in subjects with obesity ( Ley et al., 2006 ), diabetes ( Qin et al., 2012 ), cystic fibrosis ( Hayden et al., 2020 ), kidney disease ( Joossens et al., 2019 ), SAM ( Subramanian et al., 2014 ), and moderate acute malnutrition (MAM) ( Raman et al., 2019 ) compared to the compositions of healthy individuals. In clinical practice, individual somatic conditions caused by microorganisms are relatively common such as Salmonella infection ( Johari et al., 2019 ) and rabies ( Hemachudha et al., 2013 ). In regard to the growth of children, a previous study revealed the development of the gut microbiota from infancy to childhood is spontaneous ( Stewart et al., 2018 ) and synchronizes with the maturation of both the somatic body and nervous system.…”

Section: Introductionmentioning

confidence: 99%

Underdevelopment of gut microbiota in failure to thrive infants of up to 12 months of age

Zhang

Miao

et al. 2022

Front. Cell. Infect. Microbiol.

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Laboratory and clinical studies have revealed the importance of gut microbiota in children with severe pediatric pathological conditions such as severe acute malnutrition (SAM); however, under relatively milder conditions such as, failure to thrive (FTT), the role of the gut microbiota remains poorly characterized. Here, we analyzed stool samples from 54 subjects with a clinical diagnosis of failure to thrive (FTT), 49 preterm subjects with corrected normal growth (NFTT-pre), and 49 healthy subjects (NFTT) between 3-12 months of age using 16S rRNA gene sequencing. We observed that the clinical condition of FTT, age, head circumference, intrauterine growth restriction (IUGR), and feeding methods significantly affected gut microbiota. The microbiota age of subjects was significantly correlated with their anthropomorphic features, and the FTT subjects exhibited underdeveloped gut microbiota characterized by a significantly decreased microbiota-for-age Z-score (MAZ). The FTT and NFTT-pre groups exhibited an obvious disrupted developmental trajectory of gut microbiota across age, and the development of their alpha diversities and the observed OTU and Shannon indices were inadequate, particularly in subjects with FTT. Moreover, sequential colonization and enrichment of bacteria such as Bacteroides, Bifidobacterium, Streptococcus and most age-discriminatory bacterial taxa and their microbial functions were disorganized in FTT compared to that in NFTT. Our results revealed an underdevelopment of the gut microbiota in infants with failure to thrive that possesses potential clinical and practical importance.

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