Dissection of floral induction pathways using global expression analysis

Schmid, Markus; Uhlenhaut, N. Henriette; Godard, François; Demar, Monika; Bressan, Ray A.; Weigel, Detlef; Lohmann, Jan U.

doi:10.1242/dev.00842

Cited by 438 publications

(498 citation statements)

References 76 publications

Supporting

Mentioning

461

Contrasting

Unclassified

Order By: Relevance

“…GIGANTEA (GI), is an Arabidopsis clock protein that links the circadian pacemaker and the photoperiodic flowering response through interaction with COSTANT (CO) and FLOWER-ING LOCUS T (FT) (Yanovsky and Kay 2003;Mizoguchi et al 2005). Interestingly, a plant miRNA (miR-172) was identified that responds to day length (Schmid et al 2003). A recent study showed that GI regulates the response of miR-172 to change in day length (Jung et al 2007).…”

Section: Role Of Mirna In the Circadian Clockmentioning

confidence: 99%

The role of microRNAs (miRNA) in circadian rhythmicity

Pegoraro

Tauber

2008

J Genet

View full text Add to dashboard Cite

MicroRNA (miRNA) is a recently discovered new class of small RNA molecules that have a significant role in regulating gene and protein expression. These small RNAs (∼22 nt) bind to 3 untranslated regions (3 UTRs) and induce degradation or repression of translation of their mRNA targets. Hundreds of miRNAs have been identified in various organisms and have been shown to play a significant role in development and normal cell functioning. Recently, a few studies have suggested that miRNAs may be an important regulators of circadian rhythmicity, providing a new dimension (posttranscriptional) of our understanding of biological clocks. Here, we describe the mechanisms of miRNA regulation, and recent studies attempting to identify clock miRNAs and their function in the circadian system. [Pegoraro M. and Tauber E. 2008 The role of microRNAs (miRNA) in circadian rhythmicity. J. Genet. 87,[505][506][507][508][509][510][511]

show abstract

Section: Role Of Mirna In the Circadian Clockmentioning

confidence: 99%

The role of microRNAs (miRNA) in circadian rhythmicity

Pegoraro

Tauber

2008

J Genet

View full text Add to dashboard Cite

show abstract

“…In addition, LFY activates floral homeotic genes and their cofactors (Busch et al, 2001;Lamb et al, 2002;Parcy et al, 1998;Schmid et al, 2003;Weigel and Meyerowitz, 1993;William et al, 2004). These functions are generally preserved in other angiosperms, although FLO/LFY has acquired additional roles in some plants such as pea (Ahearn et al, 2001;Bomblies et al, 2003;Coen et al, 1990;Hofer et al, 1997;MolineroRosales et al, 1999;Schultz and Haughn, 1991;Souer et al, 1998;Weigel et al, 1992).…”

Section: Evolution Of Flo/lfy Genes and Body Plan In Land Plantsmentioning

confidence: 99%

Diversification of gene function: homologs of the floral regulatorFLO/LFYcontrol the first zygotic cell division in the mossPhyscomitrella patens

et al. 2005

View full text Add to dashboard Cite

After fertilization, the zygote undergoes dynamic changes in chromosomal and cytoplasmic organization, and begins the cell cycles that eventually lead to formation of the multicellular embryo. Specific transcription factors that initiate this cascade of events in land plants have not been identified. We have identified two FLO/LFY genes, PpLFY1 and PpLFY2, that regulate the first cell division after formation of the zygote in the moss Physcomitrella patens. The deduced amino acid sequences of the two PpLFY genes are 94.8% identical to each other and show similar expression patterns. While fertilization occurred in the PpLFY disruptants, the development of double disruptant zygotes was arrested at the single-cell stage. When the double disruptants, as the female parent, were crossed with the wild type, as the male parent, normal sporophytes were formed, supporting the notion that the PpLFY genes function after fertilization to regulate the first mitotic cell division in zygotes. The rare sporophytes that formed on the PpLFY double disruptants showed mostly normal organogenesis, but had abnormalities in the pattern of cell division, supporting a role of PpLFY genes in regulating cell division. The FLO/LFY genes in angiosperms are conserved master regulators of floral identity without any obvious effects on cell division. By contrast, our study suggests that FLO/LFY genes have functions throughout sporophyte development in the basal land plant lineages.

show abstract

“…Recent studies have uncovered the role of microRNAs (miRNAs), ~21-nucleotide noncoding RNAs, as regulators of floral patterning gene expression in Arabidopsis flower development (Aukerman and Sakai 2003;Schmid et al 2003;Achard et al 2004;Chen 2004;Mallory et al 2004a;Baker et al 2005;Millar and Gubler 2005). Plant miRNAs regulate cognate protein coding RNAs through either transcript cleavage or translational inhibition or both (reviewed in Bartel and Bartel 2003;Carrington and Ambros 2003;Bartel 2004;Dugas and Bartel 2004;He and Hannon 2004;Mallory and Vaucheret 2004;Kidner and Martienssen 2005;Chen 2005;Valencia-Sanchez et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Floral patterning defects induced by Arabidopsis APETALA2 and microRNA172 expression in Nicotiana benthamiana

et al. 2006

View full text Add to dashboard Cite

Floral patterning and morphogenesis are controlled by many transcription factors including floral homeotic proteins, by which floral organ identity is determined. Recent studies have uncovered widespread regulation of transcription factors by microRNAs (miRNAs), ~21-nucleotide noncoding RNAs that regulate protein-coding RNAs through transcript cleavage and/or translational inhibition. The regulation of the floral homeotic gene APETALA2 (AP2) by miR172 is crucial for normal Arabidopsis flower development and is likely to be conserved across plant species. Here we probe the activity of the AP2/miR172 regulatory circuit in a heterologous Solanaceae species, Nicotiana benthamiana. We generated transgenic N. benthamiana lines expressing Arabidopsis wild type AP2 (35S∷AP2), miR172-resistant AP2 mutant (35S∷AP2m3) and MIR172a-1 (35S∷MIR172) under the control of the cauliflower mosaic virus 35S promoter. 35S∷AP2m3 plants accumulated high levels of AP2 mRNA and protein and exhibited floral patterning defects that included proliferation of numerous petals, stamens and carpels indicating loss of floral determinacy. On the other hand, nearly all 35S∷AP2 plants accumulated barely detectable levels of AP2 mRNA or protein and were essentially non-phenotypic. Overall, the data indicated that expression of the wild type Arabidopsis AP2 transgene was repressed at the mRNA level by an endogenous N. benthamiana miR172 homologue that could be detected using Arabidopsis miR172 probe. Interestingly, 35S∷MIR172 plants had sepal-to-petal transformations and/or more sepals and petals, suggesting interference with N. benthamiana normal floral homeotic gene function in perianth organs. Our studies uncover the potential utility of the Arabidopsis AP2/miR172 system as a tool for manipulation of floral architecture and flowering time in non-model plants.

show abstract

Dissection of floral induction pathways using global expression analysis

Cited by 438 publications

References 76 publications

The role of microRNAs (miRNA) in circadian rhythmicity

The role of microRNAs (miRNA) in circadian rhythmicity

Diversification of gene function: homologs of the floral regulatorFLO/LFYcontrol the first zygotic cell division in the mossPhyscomitrella patens

Floral patterning defects induced by Arabidopsis APETALA2 and microRNA172 expression in Nicotiana benthamiana

Contact Info

Product

Resources

About