2002
DOI: 10.1590/s0103-90162002000200031 View full text |Buy / Rent full text
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Abstract: The Opaque-2 (O2) gene encodes a transcriptional activator specifically expressed for grain development of maize. o2 mutants have an opaque and chalky kernel, with a decrease in zein storage protein content, and an increase in the proportions of lysine and tryptophan. In this review, we present recent results investigating genetic properties of the O2 network, using transcriptome and proteome approaches, associated with measurements of activities of enzymes of the aspartate pathway and lysine degradation. The … Show more

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“…Through transcriptome and proteome approaches, the regulatory role of the opaque-2 gene has been confirmed, since a 3' restriction site was shown to be associated with LOR-SDH mRNA abundance (Lefèvre et al, 2002). The use of such techniques certainly will contribute significantly in the future.…”
Section: Biochemical Mutants and Transgenic Plants For The Productionmentioning
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“…Through transcriptome and proteome approaches, the regulatory role of the opaque-2 gene has been confirmed, since a 3' restriction site was shown to be associated with LOR-SDH mRNA abundance (Lefèvre et al, 2002). The use of such techniques certainly will contribute significantly in the future.…”
Section: Biochemical Mutants and Transgenic Plants For The Productionmentioning
“…This mutation is characterized by an opaque phenotype with a farinaceous endosperm. The high lysine concentration observed in the endosperm is related to an increase in the concentration of soluble lysine and storage proteins with the simultaneous reduction of the prolamin fraction, which has only trace amounts of lysine (Lefèvre et al, 2002). The introduction of the opaque phenotype modifier genes allowed the production of opaque-2 maize lines with good grain productivity, that also exhibit characteristics of high lysine and tryptophan contents, but with a translucent phenotype, which have been denominated as quality protein maize -(QPM) (Vasal, 1994;Gaziola et al, 1999).…”
Section: Biochemical Mutants and Transgenic Plants For The Productionmentioning
“…Lysine and threonine, which are in low concentrations in cereal seeds are derived from aspartate (Lefèvre et al, 2002;Toro et al, 2003). The in vitro manipulation of AK has proved to be difficult and in order to isolate and characterize the enzyme from different plant tissues, the assay used to determine enzyme activity has to be efficient.…”
Section: Resultsmentioning
“…Amino acids are the major nitrogen containing compounds of plants and are the building-blocks of proteins (Helm et al, 2004). The essential amino acids lysine, threonine, methionine and isoleucine are derived from aspartic acid in a strongly regulated metabolic pathway (Lefèvre et al, 2002;Toro et al, 2003).…”
Section: Introductionmentioning