Dissecting role of regulatory factors in NF-kappaB pathway with siRNA1

Abstract

NF‐κB, a family of related transcription factors, has been a focus of intense scientific research during the past decade. Multiple stimuli, both extracellular and intracellular, lead to its activation. The NF‐κB pathway regulates expression of a diverse array of genes involved in different biological processes. Various pathological states are characterized by the dysregulated NF‐κB pathway. Recently, NF‐κB activation has been connected with multiple aspects of oncogenesis and serves as an important mechanism to regulate cell survival in response to chemotherapy by activating different genes that inhibit apoptosis. Several methods of inhibiting NF‐κB activation, such as antisense oligonucleotides, proteosome inhibitors and RNA interference (RNAi) are currently under investigation. RNAi represents a powerful tool to better define the role of specific genes in different signal transduction pathways and has recently been used to define the function of genes that regulate the NF‐κB pathway. This review discusses the emerging role of RNAi to dissect the function of regulatory factors in the NF‐κB pathway and its potential use as a targeted therapy.

Lipid-based systemic delivery of siRNA

“…Inhibit angiogenesis via inhibiting VEGF-signaling [50–55]; 2. Inhibit tumor survival and induce apoptosis [56] via inhibiting Wnt pathway [57, 58], NF-κB pathway [59, 60], EGFR [61], Her-2/ neu [62], telomerase [63, 64], MDM2 and p53 [65, 66], or Bcl-2 [67]; 3. Enhance radio- or chemo-sensitivity by inhibit MDR1 [63, 64].…”

Effect of Reactive Oxygen Species Generation in Rabbit Corneal Epithelial Cells on Inflammatory and Apoptotic Signaling Pathways in the Presence of High Osmotic Pressure

“…NF-κB plays a key role in regulating transcription of mononuclear macrophage inflammatory response-related genes [16,17]. NF-κB could up-regulate gene transcription of TNF-a, IL-1β and other cytokines in inflammatory responses [18].…”

Perspectives of bronchial asthma treatment using small interfering RNA

“…Ген RELA (11q13) кодирует тран скрипционный фактор NF κB р65. МиРНК, нап равленные против субъединицы p65 NF κB (миРНК p65) и трансфецированные в эпителиальные клетки респираторного тракта, в экспериментах in vitro при водили к значительному снижению TNF α индуци рованного высвобождения IL 6, IL 8 [27].…”