Disruption of urogenital biofilms by lactobacilli

McMillan, Amy; Dell, Melissa; Zellar, Michelle P.; Cribby, Sarah; Martz, Sarah; Hong, Emilio; Fu, Jennifer; Abbas, Ahmed M.; Dang, Thien; Miller, Wayne L.; Reid, Gregor

doi:10.1016/j.colsurfb.2011.03.016

Cited by 127 publications

(87 citation statements)

References 35 publications

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“…It has been shown that 80% CFS from L. rhamnosus GR-1 and L. reuteri RC-14 can influence surface membrane characteristics of an UPEC isolate by upregulation of OMPs and downregulation of type 1 and P fimbriae synthesis [17]. Furthermore, non-diluted CFS from L. rhamnosus GR-1 were able to influence pre-formed UPEC biofilms by increasing the number of dead cells and decreasing cell density [19]. Antibacterial activities of Lactobacillus non-diluted CFS may thus be a decisive factor for biofilm deterioration [19].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, non-diluted CFS from L. rhamnosus GR-1 were able to influence pre-formed UPEC biofilms by increasing the number of dead cells and decreasing cell density [19]. Antibacterial activities of Lactobacillus non-diluted CFS may thus be a decisive factor for biofilm deterioration [19].…”

Section: Discussionmentioning

confidence: 99%

“…Released antibacterial substances in cell-free culture supernatants (CFS) can inhibit both the growth of different pathotypes of E. coli [14][15][16] and the expression of virulence factors [17]. In spite of the observation that in mixed biofilms lactobacilli can influence biofilm growth of other microorganisms or displace them from already formed biofilms [13,[18][19][20], the effects of secreted Lactobacillus metabolites on biofilm formation have only rarely been addressed [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

et al. 2012

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E. coli biofilms cause serious problems in medical practice by contaminating surfaces and indwelling catheters. Due to the rapid development of antibiotic resistance, alternative approaches to biofilm suppression are needed. This study addresses whether products released by antagonistic bacteria — Lactobacillus isolates from vaginal and dairy-product samples could be useful for controlling E. coli biofilms. The effects of diluted cell-free supernatants (CFS) from late-exponential Lactobacillus cultures on the growth and biofilm production of Escherichia coli were tested. Most of the CFS applied as 10−2 had no impact on bacterial growth, biofilm development however was influenced even by 10−4 of CFS. Initial screening by crystal violet assay showed that biofilm modulation varied between different CFS and E. coli combinations from inhibition to activation; however three of the tested CFS showed consistency in biofilm suppression. This was not due to antibacterial activity since Live/Dead fluorescence labeling showed insignificant differences in the amount of dead cells in control and treated samples. Some E. coli strain-specific mechanisms of response to the three CFS included reduction in hydrophobicity and motility. Released exoploysaccharides isolated from the three CFS stimulated sessile growth, but proteinase K reduced their inhibitory activities implying participation of protein or peptide biofilm suppression factor(s).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

et al. 2012

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“…McMillan et al [141] indicated that urogenital probiotic L. rhamnosus GR-1 caused a marked decrease in cell density and increased cell death of biofilms formed by pathogens associated to BV. Thus, probiotic lactobacilli could interfere with an aberrant vaginal microbiota through the eradication of pathogenic biofilms.…”

Section: Aggregation and Biofilm Formationmentioning

confidence: 99%

Profiles and technological requirements of urogenital probiotics

Nader‐Macías

Tomás

2015

Advanced Drug Delivery Reviews

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Probiotics, defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host, are considered a valid and novel alternative for the prevention and treatment of female urogenital tract infections. Lactobacilli, the predominant microorganisms of the healthy human vaginal microbiome, can be included as active pharmaceutical ingredients in probiotics products. Several requirements must be considered or criteria fulfilled during the development of a probiotic product or formula for the female urogenital tract. This review deals with the main selection criteria for urogenital probiotic microorganisms: host specificity, potential beneficial properties, functional specifications, technological characteristics and clinical trials used to test their effect on certain physiological and pathological conditions. Further studies are required to complement the current knowledge and support the clinical applications of probiotics in the urogenital tract. This therapy will allow the restoration of the ecological equilibrium of the urogenital tract microbiome as well as the recovery of the sexual and reproductive health of women.Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentin

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“…ОБЗОРЫ / REVIEWS ки [64]. Таким образом, сочетание антибакте-риальных препаратов с пробиотиками перспек-тивно в отношении лечения БВ.…”

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Current views on vaginal microbiota and its impact on pregnancy outcomes

Siniakova

Александровна

2017

Z.akus.zen.bolezn

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ВведениеНевынашивание беременности -основ-ная причина перинатальной заболеваемости и смертности во всем мире и одна из важ-нейших проблем практического акушерства.Согласно данным ВОЗ частота встречаемости данной патологии в разных странах остается стабильно высокой и составляет 10-25 % от всех выявленных беременностей (20-25 % -самопроизвольные выкидыши, 5-10 % -пре- ■ Дисбиоз влагалища -одна из ведущих причин неблагоприятных исходов беременности. Основная роль в под-держании постоянства микробиоценоза влагалища принадлежит лактобациллам. Инфекционный процесс мо-гут вызвать как патогенные, так и условно-патогенные микроорганизмы, однако в подавляющем большинстве случаев возбудителями генитальной инфекции являются представители нормальной микрофлоры. Нарушение микробиоценоза влагалища в сочетании с несостоятельностью цервикального барьера ведет к миграции бак-терий в амниотическую полость и запуску патологических механизмов, приводящих к прерыванию беремен-ности. В статье представлены современные данные о роли инфекционного фактора в генезе невынашивания беременности. Рассмотрен вопрос о способности Lactobacillus iners предрасполагать к развитию патологической влагалищной микрофлоры. Уделено внимание основным подходам к профилактике и лечению дисбиотических нарушений. Затронут вопрос о применении бактериофагов в акушерстве и гинекологии.■ Ключевые слова: микробиоценоз влагалища; преждевременные роды; самопроизвольный выкидыш; бактери-альный вагиноз; аэробный вагинит. CURRENT VIEWS ON VAGINAL MICROBIOTA AND ITS IMPACT ON PREGNANCY OUTCOMES © A.A. SiniakovaThe ■ Vaginal dysbiosis is one of the leading causes of adverse pregnancy outcomes. The main role in maintaining the constancy of vaginal microbiocenosis belongs to lactobacilli. The infection process can be caused by both pathogenic and opportunistic microorganism, however, in the vast majority of cases vaginal infections is associated with the normal microflora. Violation of microbiocenosis of the vagina in combination with a cervical barrier failure leads to migration of bacteria in the amniotic cavity and the launch of the pathological mechanisms leading to pregnancy loss. The paper presents current data on the influence of vaginal infection on pregnancy loss. Consider the ability of Lactobacillus iners predispose to the development of pathological vaginal microflora. Attention was paid to the basic approaches to prevention and treatment of dysbiotic disorders. Discussion on the use of bacteriophages in obstetrics and gynecology.

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Disruption of urogenital biofilms by lactobacilli

Cited by 127 publications

References 35 publications

Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

Profiles and technological requirements of urogenital probiotics

Current views on vaginal microbiota and its impact on pregnancy outcomes

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