“…A primary role in the network governing nitrate uptake, assimilation, storage, and distribution among different plant tissues and organs is played by two protein families, the low-affinity nitrate transporter peptide family (NPF; LATS > 0.5 mM) and the high-affinity nitrate transporter system (NRT2; HATS < 0.5 mM). NPF is a large plant family consisting of 53, 93, and 86 members in Arabidopsis thaliana, Oryza sativa, and Lotus japonicus, respectively (Tsay et al, 2007;Léran et al, 2014;Sol et al, 2019). To date, nitrate transport activity has been reported for 17 A. thaliana NPF proteins (Corratgé-Faillie and Lacombe, 2017), with AtNPF6.3 being the only exception as this displays a switching dual nitrate affinity, controlled via the phosphorylation of the threonine residue at position 101, in response to fluctuating external concentrations (Liu et al, 1999;Liu and Tsay, 2003;Parker and Newstead, 2014;Sun et al, 2014).…”