Disruption of the chitin synthase gene CHS1 from Fusarium asiaticum results in an altered structure of cell walls and reduced virulence

Type II myosin gene in Fusarium graminearum is required for septation, development, mycotoxin biosynthesis and pathogenicity

“…Mycelia were collected and lyophilized before weighing. Perithecial development was examined on autoclaved wheat kernels as previously described (Liang and Wang, 1981;Xu et al, 2010). Conidiation was measured by inoculating 15 ml CMC with 10 ll macroconidial spores (5 Â 10 5 ml À1 ) and shaking at 28°C under illumination for 14 days.…”

“…The hyphae were fixed in 2.5% (v/v) glutaraldehyde and 1% (v/v) osmium tetroxide. Sections were prepared and visualized using a H-7650 transmission electron microscope (Hitachi, Tokyo, Japan) as described by Xu et al (2010). Ultrastructural images were used to measure cell wall thickness with NIS Elements software v3.22.…”

An antibody that confers plant disease resistance targets a membrane‐bound glyoxal oxidase in Fusarium

“…The experiment was repeated three times. Escherichia coli strain XL1-Blue and Agrobacterium tumefaciens strain EHA105 were grown as previously described (Xu et al, 2010).…”

“…Virulence assays of the Fg strains were performed via single floret injection with wheat cultivar Huamai13 as previously described (Xu et al, 2010). Thirty spikes were inoculated for each strain and the number of spikelets with FHB symptoms was counted 21 d post inoculation (dpi).…”

“…Disruption, complementation, overexpression and green fluorescent protein tag expression of the GLX gene in Fusarium fungi Deletion of the GLX gene from two Fg strains (Fg 5035 and Fg 54606), one Fv strain (Fv 5611) and one Fo strain (Fo 297) was achieved by homologous recombination with strain-appropriate disruption vectors, as previously described (Xu et al, 2010) (Supporting Information Table S1; Fig. S1).…”