Disruption of Kidney Metabolism in Rats after Subchronic Combined Exposure to Low-Dose Cadmium and Chlorpyrifos

Xu, Mingyuan; Wang, Pan; Sun, Ying-Jian; Wu, Yi‐Jun

doi:10.1021/acs.chemrestox.8b00219

Cited by 20 publications

(10 citation statements)

References 44 publications

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“…A large number of metabolomic studies have employed animal models or cell lines to study the toxicity of different pesticides. ,− In recent years, metabolomics has also been applied to study the effect of OP, PYR, or OC pesticide exposure. For example, a cross-sectional study of 83 pregnant women characterizing the urine metabolome found that pesticide mixtures increased oxidative stress and disturbed energy metabolism, while a larger study of 750 pregnant women identified mitochondrial catabolic pathways as being associated with low-level exposure to OCs .…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Metabolomic Assessment of Pesticide Exposure in Central Valley, California

Paul

Walker

et al. 2021

Chem. Res. Toxicol.

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Pesticides are widely used in the agricultural Central Valley region of California. Historically, this has included organophosphates (OPs), organochlorines (OCs), and pyrethroids (PYRs). This study aimed to identify perturbations of the serum metabolome in response to each class of pesticide and mutual associations between groups of metabolites and multiple pesticides. We conducted high-resolution metabolomic profiling of serum samples from 176 older adults living in the California Central Valley using liquid chromatography with high-resolution mass spectrometry. We estimated chronic pesticide exposure (from 1974 to year of blood draw) to OPs, OCs, and PYRs from ambient sources at homes and workplaces with a geographic information system (GIS)-based model. Based on partial least-squares regression and pathway enrichment analysis, we identified metabolites and metabolic pathways associated with one or multiple pesticide classes, including mitochondrial energy metabolism, fatty acid and lipid metabolism, and amino acid metabolism. Utilizing an integrative network approach, we found that the fatty acid β-oxidation pathway is a common pathway shared across all three pesticide classes. The disruptions of the serum metabolome suggested that chronic pesticide exposure might result in oxidative stress, inflammatory reactions, and mitochondrial dysfunction, all of which have been previously implicated in a wide variety of diseases. Overall, our findings provided a comprehensive view of the molecular mechanisms of chronic pesticide toxicity, and, for the first time, our approach informs exposome research by moving from macrolevel population exposures to microlevel biologic responses.

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Section: Introductionmentioning

confidence: 99%

High-Resolution Metabolomic Assessment of Pesticide Exposure in Central Valley, California

Paul

Walker

et al. 2021

Chem. Res. Toxicol.

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“…The abuse of Chl could cause environmental pollution including water contamination, soil degradation, accumulation in crops and potential risk to animals [1 − 3]. Besides, exposure to Chl also has great threat to human health, such as affect male reproduction [4], neurotoxicity [5], nephrotoxicity [6], induce the proliferation of breast cancer cells [7,8] and acute toxicity [9]. Thus, the toxicity of Chl to environment and humans lead to urgently exploit sensitive, simple, low cost and on-site rapid methods for Chl analysis and detection, since the instrumental techniques (high performance liquid chromatography [10,11], gas chromatography [12], mass spectrometry [13], surface enhanced Raman scattering [14], etc.)…”

Section: Discussionmentioning

confidence: 99%

A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection

Liu¹,

Yang³

et al. 2021

Preprint

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Background Chlorpyrifos (Chl) is an organophosphorus pesticide, which has toxicity to environment, animals and human beings. To overcome the shortages of traditional detection methods of small molecular, this study aimed to develop a sensitive, simple, low cost and on-site rapid method for Chl analysis and detection. Thus, we developed a simple label-free gold nanoparticles (AuNPs) based colorimetric biosensor aptasensor for Chl detection using an aptamer as the caputure probe. Results The Chl-aptamer with low dissociation constant (Kd) of 58.59 ± 6.08 (nM) was selected by ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for AuNPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately thus a self-aggregation of AuNPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the limit of detection as low as 14.46 nM. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, which showed satisfying results with excellent recovery values between 96.2% and 105.6% and acceptable RSD values below 5%. Conclusions The developed colorimetric aptasensor can serve as a promising candidate for Chl detection in the area of biosensors, which also showed a great potential in simple, cheap and rapid detection of Chl.

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“…For histopathological analysis, the kidney and liver tissues of the rats were dissected immediately at the end of the 90-day exposure to the pesticide, and then were fixed in a solution of 10% formalin. After fixation, the specimens were dehydrated with 80, 95, 100% gradient alcohol, and then soaked in melted wax, and finally embedded in paraffin [ 25 ]. After that, the paraffin blocks were cut into 4-μm-thickness sections using a microtome (Microm HM 340E, Thermo Fisher Scientific, USA), then were stained with hematoxylin and eosin.…”

Section: Methodsmentioning

confidence: 99%

Long-term low-dose exposure of permethrin induces liver and kidney damage in rats

Sun

Liang

Lin

et al. 2022

BMC Pharmacol Toxicol

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Background Permethrin is one of the pyrethroid insecticides, which is widely used in agriculture and public health. Although acute toxicity of the insecticide has been studied, the chronic toxicity upon the long-term exposure has not been clear yet. The purpose of the current study is to investigate the organ toxicities of permethrin following its long-term low-dose exposure. Methods Male Wistar rats were daily administrated orally with permethrin (75 mg/kg body weight/day, gavage) for 90 days, and then the samples of biofluids (blood and urine) and organs including liver and kidney were collected. The serum and urine samples were measured by biochemical assay and the tissues of kidney and liver were examined and analyzed by histopathological method. Results The results showed that no change was found in serum and urine biochemical parameters for the toxicity; however, significant changes including hyperchromatic nuclei swollen in the hepatic parenchymal cells and the swelling proximal tubules in the kidneys were observed in the tissue structures of liver and kidneys in the histopathological sections. Conclusion These results indicate that low-dose long-term exposure of permethrin can cause chronic toxicity with slight liver and kidney damage.

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Disruption of Kidney Metabolism in Rats after Subchronic Combined Exposure to Low-Dose Cadmium and Chlorpyrifos

Cited by 20 publications

References 44 publications

High-Resolution Metabolomic Assessment of Pesticide Exposure in Central Valley, California

High-Resolution Metabolomic Assessment of Pesticide Exposure in Central Valley, California

A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection

Long-term low-dose exposure of permethrin induces liver and kidney damage in rats

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