Disruption of <i>Arabidopsis thaliana MYB26</i> results in male sterility due to non‐dehiscent anthers

Steiner-Lange, Sabine; Unte, Ulrike S.; Eckstein, Luca; Yang, Caiyun; Wilson, Zoe A.; Schmelzer, Elmon; Dekker, Koen; Saedler, Heinz

doi:10.1046/j.1365-313x.2003.01745.x

Cited by 184 publications

(126 citation statements)

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“…Similar observations were made in terms of organ-specific expression patterns: AtMYB7, AtMYB44 and AtMYB73 were expressed in all plant organs studied while AtMYB46 was only detected in siliques and AtMYB21 only in flower buds (Shin et al 2002). Several MYB-related genes expressed in anthers have been identified, namely AtMYB26 and AtMYB103 in Arabidopsis (Steiner-Lange et al 2003;Higginson et al 2003) and NtMYBAS1 in tobacco . In Arabidopsis, AtMYB103 gene expression was restricted to the tapetum of developing anthers and trichomes, down-regulation results in early tapetal degeneration and aberrant pollen.…”

Section: Evolution Of Mybsupporting

confidence: 79%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

824

544

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Regulation of gene expression at the level of transcription controls many crucial biological processes. Transcription factors (TFs) play a great role in controlling cellular processes and MYB TF family is large and involved in controlling various processes like responses to biotic and abiotic stresses, development, differentiation, metabolism, defense etc. Here, we review MYB TFs with particular emphasis on their role in controlling different biological processes. This will provide valuable insights in understanding regulatory networks and associated functions to develop strategies for crop improvement.

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Section: Evolution Of Mybsupporting

confidence: 79%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

824

544

View full text Add to dashboard Cite

show abstract

“…However, the lignification of tissues contiguous to those that will experience cell separation appears to be required if abscission is to occur. That is the case for the lignification of the endothecium and the valve margin cells, which are essential to provide the mechanical force needed for anther dehiscence and pod shattering, respectively, in Arabidopsis (Steiner-Lange et al, 2003;Mitsuda and Ohme-Takagi, 2008).…”

Section: Osh15-qsh1 Dimer Functions For Az Developmentmentioning

confidence: 99%

KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes

et al. 2017

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Seed shattering is an agronomically important trait. Two major domestication factors are responsible for this: qSH1 and SH5. Whereas qSH1 functions in cell differentiation in the abscission zone (AZ), a major role of SH5 is the repression of lignin deposition. We have determined that a KNOX protein, OSH15, also controls seed shattering. Knockdown mutations of OSH15 showed reduced seed-shattering phenotypes. Coimmunoprecipitation experiments revealed that OSH15 interacts with SH5 and qSH1, two proteins in the BELL homeobox family. In transgenic plants carrying the OSH15 promoter-GUS reporter construct, the reporter gene was preferentially expressed in the AZ during young spikelet development. The RNA in situ hybridization experiment also showed that OSH15 messenger RNAs were abundant in the AZ during spikelet development. Analyses of osh15 SH5-D double mutants showed that SH5 could not increase the degree of seed shattering when OSH15 was absent, indicating that SH5 functions together with OSH15. In addition to the seedshattering phenotype, osh15 mutants displayed dwarfism and accumulated a higher amount of lignin in internodes due to increased expression of the genes involved in lignin biosynthesis. Knockout mutations of CAD2, which encodes an enzyme for the last step in the monolignol biosynthesis pathway, caused an easy seed-shattering phenotype by reducing lignin deposition in the AZ. This indicated that the lignin level is an important determinant of seed shattering in rice (Oryza sativa). Chromatin immunoprecipitation assays demonstrated that both OSH15 and SH5 interact directly with CAD2 chromatin. We conclude that OSH15 and SH5 form a dimer that enhances seed shattering by directly inhibiting lignin biosynthesis genes.

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“…Genetic analyses have led to the isolation of several mutants with distinct defects in stamen development (Dawson et al, 1993;Sanders et al, 1999;Schiefthaler et al, 1999;Yang et al, 1999;Canales et al, 2002;Zhao et al, 2002;Reddy et al, 2003;Sorensen et al, 2003;Steiner-Lange et al, 2003;Albrecht et al, 2005). Cloning of the genes affected in these mutants resulted in the identification of key regulators of stamen formation.…”

mentioning

confidence: 99%

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

et al. 2007

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To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.

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Disruption of Arabidopsis thaliana MYB26 results in male sterility due to non‐dehiscent anthers

Cited by 184 publications

References 50 publications

MYB transcription factor genes as regulators for plant responses: an overview

MYB transcription factor genes as regulators for plant responses: an overview

KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

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