Disposable electrochemical immunosensor for carcinoembryonic antigen using ferrocene liposomes and MWCNT screen-printed electrode

Subramanian, Venkataraman; Rani, C. Nirmala; Anand, Annadurai Vijay; Ho, Ja‐an Annie

doi:10.1016/j.bios.2008.10.006

Cited by 152 publications

(88 citation statements)

References 32 publications

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“…Thus the generation of -OH and -COOH groups on MWCNTs due to functionalization was observed (Supporting information Fig. S1) [30]. In order to prevent the MWCNT from aggregating into clumps, they were enclosed in a partially polyallylamine matrix.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical immunosensor for multiplexed detection of food-borne pathogens using nanocrystal bioconjugates and MWCNT screen-printed electrode

Subramanian

Rani²,

2012

Talanta

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150

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a b s t r a c tBacterial food poisoning is an ever-present threat that can be prevented with proper care and handling of food products. A disposable electrochemical immunosensor for the simultaneous measurements of common food pathogenic bacteria namely Escherichia coli O157:H7 (E. coli), campylobacter and salmonella were developed. The immunosensor was fabricated by immobilizing the mixture of anti-E. coli, anticampylobacter and anti-salmonella antibodies with a ratio of 1:1:1 on the surface of the multiwall carbon nanotube-polyallylamine modified screen printed electrode (MWCNT-PAH/SPE). Bacteria suspension became attached to the immobilized antibodies when the immunosensor was incubated in liquid samples. The sandwich immunoassay was performed with three antibodies conjugated with specific nanocrystal (␣-E. coli-CdS, ␣-campylobacter-PbS and ␣-salmonella-CuS) which has releasable metal ions for electrochemical measurements. The square wave anodic stripping voltammetry (SWASV) was employed to measure released metal ions from bound antibody nanocrystal conjugates. The calibration curves for three selected bacteria were found in the range of 1 × 10 3 -5 × 10 5 cells mL −1 with the limit of detection (LOD) 400 cells mL −1 for salmonella, 400 cells mL −1 for campylobacter and 800 cells mL −1 for E. coli. The precision and sensitivity of this method show the feasibility of multiplexed determination of bacteria in milk samples.

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Section: Resultsmentioning

confidence: 99%

Electrochemical immunosensor for multiplexed detection of food-borne pathogens using nanocrystal bioconjugates and MWCNT screen-printed electrode

Subramanian

Rani²,

2012

Talanta

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150

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“…Nevertheless, screen printed electrodes have been preferred due to their low cost, easy manufacture and suitability for mass production. 47 Although there is a preference for disposable electro-immuno sensors, [48][49][50] there is a growing trend of surface renewal for reusable purposes. 51 However, the latter involves long renewal times, which increases the response time of the system.…”

Section: Electro-immuno Sensorsmentioning

confidence: 99%

Electro-Immuno Sensors: Current Developments and Future Trends

Barbosa¹,

Segura²,

Osma³

2017

IJBSBE

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Abbreviations: Iot, internet of things; ELISA, enzyme-linked immuno sorbent assay; WB, western blot; IHC, immunohistochemistry; ICC, immunocytochemistry; FACS, fluorescence-activated cell sorting; IP, immuno precipitation; ELISPOT, enzyme-linked immuno spot; PSA, prostate specific antigen; IDEs, interdigitated electrodes; HPV, human papilloma virus IntroductionBiosensors have been mostly used in the food industry, medical and biological fields, among others, where physical and chemical sensors cannot accurately measure all variables. Biosensors are usually comprised by a biological recognition element and a transducer.1,2 Among the different commercial biosensors, the most common recognition elements are DNA, 3 enzymes, 4 aptamers 5,6 and antibodies.7,8 DNA-based biosensors detect specific analytes by their cleavage and ligation to a functionalized DNA strand.9 Enzymatic biosensors immobilize an enzyme to an electrode to facilitate the transport of electrons from the enzyme to the electrode, produced by specific reactions at the active site of the enzyme.2 Aptamers are chemically synthesized oligonucleotides 10 that provide high specificity and sensitivity towards a specific target. Antibody-based biosensors recognize a specific antigen from the immobilization of a monoclonal or polyclonal antibody.8 In general, transducers vary according to the end user towards which the sensor is thought. Among others, transducers include electrochemical responses, mass changes, optical absorption or transmission, and thermal readings. During the last decades, several immunoassay techniques have appeared as a response to the need of having specific and sensitive tests for antigen recognition. The methods by which the recognition is perform varies from one to another, but the presence of at least one recognition antibody remains as a constant. Among others, the most common immune tests used in laboratories and medical facilities are the enzyme-linked immuno sorbent assay (ELISA), western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), fluorescence-activated cell sorting (FACS), immuno precipitation (IP), and enzyme-linked immuno spot (ELISPOT). Figure 1 shows a general diagram of these immunoassays.ELISA is a widely used technique for the recognition of antibodies' antigens within a sample by means of the biochemical recognition of an enzyme.11 In this technique, specific antibodies are conjugated to an enzyme that catalyzes a colorimetric molecule. Spectrophotometric measurements are used to determine the antigen concentration in the sample. ELISA tests can be performed in a qualitative way, in which the results are positive or negative by statistical methods; or quantitative, in which a standard curve from spectrophotometric measurements can be used to quantify the concentration of the antigen. 12WB is a technique for the detection of specific proteins in a sample through the electrophoretic transfer from a separating gel to a blotting matrix.13 WB uses available monoclonal or polyclonal antibodies for the d...

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“…Several disposable electrochemical immunosensors using different electroactive materials such as conducting polymers, 43 graphite, 44 carbon nanotubes, 45 or metals 46 47 Delivered by Publishing 50 and Viswanathan et al 51 for the detection of the carcinoembryonic antigen, a tumor marker. In the former, the authors have proposed a sensitive electrochemical immunosensor based on a carbon nanoparticle-modified screen-printed graphite electrode covered with specific antibodies, using CdS nanocrystals as biotracers as a strategy for signal amplification.…”

Section: Disposable Immusensorsmentioning

confidence: 99%

Disposable Biosensors for Clinical Diagnosis

Janegitz¹,

Cancino²,

Zucolotto³

2014

j. nanosci. nanotech.

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We present an overview on the use of disposable electrochemical biosensors for diagnostics, focusing on the applications of these devices as immunosensors and DNA sensors in the point-of-care diagnostics. Analytical biosensors have emerged as efficient alternatives for the detection of innumerous diseases, because of their high specificity and the convenience of detecting the electrochemical signals produced by the presence of an analyte using a portable equipment. This review highlights the recently developed strategies toward immobilization of different biological molecules on disposable electrodes such as carbon nanotubes and metal nanoparticles. In the course of the review, we first introduced the disposable biosensors, followed by an overview of the immunosensors, and discussed the applications of DNA sensors and disposable biosensors in point-of-care diagnostics. We also have evaluated the prospects and future applications of these devices in the field of biomedical research.

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Disposable electrochemical immunosensor for carcinoembryonic antigen using ferrocene liposomes and MWCNT screen-printed electrode

Cited by 152 publications

References 32 publications

Electrochemical immunosensor for multiplexed detection of food-borne pathogens using nanocrystal bioconjugates and MWCNT screen-printed electrode

Electrochemical immunosensor for multiplexed detection of food-borne pathogens using nanocrystal bioconjugates and MWCNT screen-printed electrode

Electro-Immuno Sensors: Current Developments and Future Trends

Disposable Biosensors for Clinical Diagnosis

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