Disposable biosensors for determination of biogenic amines

High-throughput sensing microtiter plate for determination of biogenic amines in seafood using fluorescence or eye-vision

“…Therefore, the BA content found in the real samples was related to the histamine concentration and was expressed in equivalents of histamine, similarly, as was done by others. 23 This total amount of amines (TAC) was quantified by spiking 20,22,[34][35][36] standard solutions of histamine (5, 10, 20, 30, 40 and 50 mg mL À1 ) to the diluted extracts of seafood sample. A comparison of the slopes of the calibration plot of histamine and the spiked sample of fresh codfish revealed almost identical slopes (data not shown).…”

“…Disposable screen-printed electrode biosensors with enzymes have been shown as a step forward to reduced sample pretreatment. 23 Another approach uses a home-built reflectometric sensing system to monitor the total volatile amines in the headspace over fish samples. 24,25 The color change shown at various pH has potential to be applied in food packaging sensors.…”

Electrode Materials (Bulk Materials and Modification)

“…438 In this sense, DNA oligonucleotides have been attached to carbon, 439,440 diamond 441,442 and silicon. 443 Two strategies have been developed to this aim. A first strategy is based on the attachment of the activated nucleotides to diazonium-modified substrates 439,443 whereas a second approach involves the diazotization of the aminophenyl groups of the analyte which is electrochemically addressed to the substrate.…”

“…443 Two strategies have been developed to this aim. A first strategy is based on the attachment of the activated nucleotides to diazonium-modified substrates 439,443 whereas a second approach involves the diazotization of the aminophenyl groups of the analyte which is electrochemically addressed to the substrate. 444 Analogous procedures have been employed for the development of other biosensors, mainly based on the attachment of proteins and enzymes to surfaces.…”

An Extended-gate Type Organic FET Based Biosensor for Detecting Biogenic Amines in Aqueous Solution

“…2 Therefore, the detection of biogenic amines is considered very meaningful in medicinal and pharmaceutical chemistry, as well as food science. To date, the detection of biogenic amines has relied on high-performance liquid chromatography (HPLC), 3 electrochemical sensors [4][5][6][7] or fluorescent probes. [8][9][10][11] Although these analytical methods are very accurate and reliable, the development of low-cost and easy-to-use sensor devices for the monitoring of biogenic amine concentrations has become necessary.…”