Disposable amperometric immunosensor for simple and sensitive determination of aflatoxin B 1 in wheat

Ma, Haihua; Sun, Jifei; Zhang, Yuan; Xia, Shanhong

doi:10.1016/j.bej.2016.08.003

Cited by 38 publications

(11 citation statements)

References 42 publications

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“…The immunosensor stability was assessed by measuring response currents after three weeks of storage at 4 • C. The results revealed that immunosensor maintained 90% of its initial current signal, which indicates its long-term stability for anti-AFB1 detection. The immunosensor had similar performance to others reported in the literature, as shown in Table 1 [39,40,53,54]. As expected, the limit of detection of our immunosensor is not as low as in some of the previous reports, but it is advantageous because the sensing system was fabricated through a simple and low-cost process combined to a novel conductive ink formulation.…”

Section: Electrochemical Detection Of Mycotoxin Afb1supporting

confidence: 83%

“…For the immobilization of anti-AFB1, a solution of EDC (0.8 M) and NHS (0.2 M) in 0.1 mol•L −1 MES buffer was dripped onto the modified electrode. This solution was maintained in contact with the electrode for 40 min at 25 • C to activate the amine groups from chitosan and the carboxylic groups from MWCNTs [38,39]. After rinsing the electrode with 10 mM PBS (pH 7.4) and drying at room temperature, 10 µL of anti-AFB1 solution (100 µg/mL) in 0.1 mol•L −1 MES buffer were deposited onto the modified working electrode and left for 40 min at 25 • C. Next, the electrode was washed with PBS to remove non-immobilized antibodies and dried.…”

Section: Functionalization Of Printed Electrodes and Antibody Immobil...mentioning

confidence: 99%

“…An aliquot of 10 mL of the supernatant was diluted with 30 mL PBS and filtered with glass fiber. The extracts were spiked with AFB1 at concentrations varying from 1 to 30 ng mL −1 [39].…”

Section: Application Of the Immunosensors For Real Food Sample Analysismentioning

confidence: 99%

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Design of A Low-Cost and Disposable Paper-Based Immunosensor for the Rapid and Sensitive Detection of Aflatoxin B1

et al. 2020

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We report a paper-based electrochemical immunosensor made with sustainable materials to detect aflatoxin B1 (AFB1), a highly toxic, carcinogenic mycotoxin found in food. The immunosensor was prepared with a waterproof paper substrate and low-cost graphite-based conductive ink through a simple cut-printing method. The working electrode was functionalized with a drop-cast film of multiwalled carbon nanotubes (MWCNT)/chitosan on which a layer of anti-AFB1 monoclonal antibodies was immobilized covalently. The architecture of the immunosensor was confirmed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and electrochemical impedance spectroscopy (EIS), including the effective immobilization of the active layer of anti-AFB1. With EIS as the principle of detection, the immunosensor could detect AFB1 in the range from 1 to 30 ng·mL−1, and detection limit of 0.62 ng·mL−1. This sensitivity is sufficient to detect AFB1 in food according to regulatory agencies. The immunosensor exhibited good repeatability, reproducibility, stability, and selectivity in experiments with a possible interferent. Furthermore, detection of AFB1 in maize flour samples yielded recovery of 97–99%, in a demonstration of the possible use of the paper-based immunosensor to detect AFB1 using extraction solutions from food samples.

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Section: Electrochemical Detection Of Mycotoxin Afb1supporting

confidence: 83%

Section: Functionalization Of Printed Electrodes and Antibody Immobil...mentioning

confidence: 99%

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Design of A Low-Cost and Disposable Paper-Based Immunosensor for the Rapid and Sensitive Detection of Aflatoxin B1

et al. 2020

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“…2. The AuNPs/chitosan nanocomposite prepared by onestep electrodeposition 48 provided plentiful amine groups, high surface energy and excellent biocompatibility for antibody immobilization owing to its porous three-dimensional morphology increasing the effective surface of microelectrode. The disposable and label-free immunosensor obtained the linear range from 1.6-32 ng mL −1 in wheat.…”

Section: Biosensors Modified With Binary Nanocompositementioning

confidence: 99%

“…Fabrication processes of two immunosensors using AFB1 antibody (Yb) for the detection of AFB1. (A) AuNPs/Cu-apatite/Yb; 49 (B) AuNPs/chitosan nanocomposite 48.…”

mentioning

confidence: 99%

Review—Electrochemical Sensors and Biosensors Modified with Binary Nanocomposite for Food Safety

Lü

Zhu

et al. 2019

J. Electrochem. Soc.

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Binary nanocomposite has become an excellent modification material for sensors and biosensors, which is superior to single nanomaterial. Multiple electrochemical sensors with four types of binary nanocomposites and biosensors with four biological elements were presented in the application of food safety. Different sensors for the detection of the same food analyte were contrasted. The synergistic effects and interactions of nanocomposites, and analytical performance of modified (bio)sensors are elaborated. The future perspectives for binary nanocomposites used on food (bio)sensors are discussed.

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Chitosan for Sensors and Electrochemical Applications

Silva

Batista²,

Santin

2019

Chitin and Chitosan

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Disposable amperometric immunosensor for simple and sensitive determination of aflatoxin B 1 in wheat

Cited by 38 publications

References 42 publications

Design of A Low-Cost and Disposable Paper-Based Immunosensor for the Rapid and Sensitive Detection of Aflatoxin B1

Design of A Low-Cost and Disposable Paper-Based Immunosensor for the Rapid and Sensitive Detection of Aflatoxin B1

Review—Electrochemical Sensors and Biosensors Modified with Binary Nanocomposite for Food Safety

Chitosan for Sensors and Electrochemical Applications

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