Dispersible MoS2 micro-sheets induced a proinflammatory response and apoptosis in the gills and liver of adult zebrafish

Yu, Yadong; Yi, Yanliang; Li, Yangying; Peng, Ting; Lao, Shanli; Zhang, Jiahao; Liang, Shaocui; Xiong, Yan; Shao, Shasha; Wu, Na; Zhao, Ye; Huang, He

doi:10.1039/c8ra00922h

Cited by 16 publications

(12 citation statements)

References 45 publications

(60 reference statements)

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“…Yu et al demonstrated that chitosan functionalized MSNs induced ROS accumulation in human dermal cells 37 and in the gills and liver of adult zebrafish. 38 Consistently, Pardo et al 39 found that fullerene-like MSNs could induce robust protective antioxidant/detoxification defenses in human bronchial cells, indicating ROS generation in this cell line. Using E. coli as the model organism, Wu et al 40 and Qureshi et al 41 proved that the MSN exposure at a concentration of 100-1000 μg/mL dramatically increased the intracellular concentrations of ROS in bacteria.…”

Section: Ros-induced Cytoskeleton Arrangement Participates In the Impmentioning

confidence: 77%

Immunostimulatory Potential of MoS2 Nanosheets: Enhancing Dendritic Cell Maturation, Migration and T Cell Elicitation

Deng

Pan

Sun

et al. 2020

IJN

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Background: Due to their extraordinary physical and chemical properties, MoS 2 nanosheets (MSNs) are becoming more widely used in nanomedicine. However, their influence on immune systems remains unclear. Materials and Methods: Two few-layered MSNs at sizes of 100-250 nm (S-MSNs) and 400-500 nm (L-MSNs) were used in this study. Bone marrow-derived dendritic cells (DCs) were exposed to both MSNs at different doses (0, 8, 16, 32, 64, 128 µg/mL) for 48 h and subjected to analyses of surface marker expression, cytokine secretion, lymphoid homing and in vivo T cell priming. Results: Different-sized MSNs of all doses did not affect the viability of DCs. The expression of CD40, CD80, CD86 and CCR7 was significantly higher on both S-MSN-and L-MSN-treated DCs at a dose of 128 μg/mL. As the dose of MSN increased, the secretion of IL-12p70 remained unchanged, the secretion of IL-1β decreased, and the production of TNF-α increased. A significant increase in IL-6 was observed in the 128 µg/mL L-MSNtreated DCs. In particular, MSN treatment dramatically improved the ex vivo movement and in vivo homing ability of both the local resident and blood circulating DCs. Furthermore, the cytoskeleton rearrangement regulated by ROS elevation was responsible for the enhanced homing ability of the MSNs. More robust CD4 + and CD8 + T cell proliferation and activation (characterized by high expression of CD107a, CD69 and ICOS) was observed in mice vaccinated with MSN-treated DCs. Importantly, exposure to MSNs did not interrupt LPSinduced DC activation, homing and T cell priming. Conclusion: Few-layered MSNs ranging from 100 to 500 nm in size could play an immunostimulatory role in enhancing DC maturation, migration and T cell elicitation, making them a good candidate for vaccine adjuvants. Investigation of this study will not only expand the applications of MSNs and other new transition metal dichalcogenides (TMDCs) but also shed light on the in vivo immune-risk evaluation of MSN-based nanomaterials.

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Section: Ros-induced Cytoskeleton Arrangement Participates In the Impmentioning

confidence: 77%

Immunostimulatory Potential of MoS2 Nanosheets: Enhancing Dendritic Cell Maturation, Migration and T Cell Elicitation

Deng

Pan

Sun

et al. 2020

IJN

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“…[ 93 ] Furthermore, Yu et al have demonstrated the induction of pro‐inflammatory and apoptotic responses (e.g., IL‐1β, IL‐6, and AIF gene) in the livers of adult zebrafish by chitosan‐functionalized MoS 2 micro‐sheets. [ 150 ] Thus, much attention should be paid to the adverse effects induced by MoS 2 NMs for developing safer nanoapplications.…”

Section: Cell Type‐specific Responses and Molecular Mechanismsmentioning

confidence: 99%

Understanding Nanomaterial–Liver Interactions to Facilitate the Development of Safer Nanoapplications

Chen

Xia

2022

Advanced Materials

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vaccines, and drug carriers (Figure 1). The functional advantages of NMs are derived from their unique physical and chemical properties. [1,[4][5][6] Among all the NMs, metallic NMs including metal and metal oxides (MOx) nanoparticles (NPs), such as Ag and Au NPs, transition-metal oxides (TMOs, e.g., SiO 2 , Co 3 O 4 , and Mn 2 O 3 ), and rare-earth oxides REOs (e.g., Gd 2 O 3 , La 2 O 3 , and Y 2 O 3 ) NPs, are among the most produced NMs worldwide. Also, they are widely used in consumer products such as dietary supplements, fuel additives, cosmetics, bioimaging, and drug carriers, etc. due to their properties such as small size, high surface area, controlled particle shape as well as superior mechanical, electronic, optical, and magnetic properties. [7][8][9] For example, SiO 2 NPs have been applied in the theranostic fields, including bioimaging and targeted drug delivery. [7] In addition, the popularity of carbon nanomaterials (CNMs) including 0D fullerenes, 1D carbon nanotubes (CNTs), and 2D graphene-based nanoparticles (GBN) including graphene, graphene oxide (GO), and reduced graphene oxide (rGO) has been on the rise for their applications in battery, electronics, drug delivery, bio-sensing, bio-imaging, and tissue engineering due to their large surface area, diverse surface functional groups, excellent optical property, and efficient drug-loading capacity. [10][11][12][13][14][15][16] For example, an advanced NMsbased biosensing platform based on rGO has been developed to detect the coronavirus disease 2019 (COVID-19) antibodies within seconds. [17] Additionally, a form of nanostructured cellulose (nanocellulose), including cellulose nanocrystal (CNC) and cellulose nanofiber (CNF) has been increasingly considered for applications in papermaking, coatings, food, nanocomposite formulations and reinforcement, and biomedical fields (e.g., wound dressing) due to its biocompatibility, outstanding mechanical, chemical, and rheological properties. [18,19] Also 2D transition metal dichalcogenides (TMDs) (including MoS 2 and BN) with high surface area and free surface energy levels are increasingly being used for commercial applications in energy generation, sensors, catalysis, electronics, and biomedicine fields. [13,[20][21][22] Similarly, organic NPs (including lipid, liposome, polymer, micelle, dendrimer, and protein/peptide-based NPs) have been widely used in diagnosis, drug delivery, bioimaging, and cancer therapy because of their facile synthesis and chemical modification, self-assembly, biocompatibility, and biodegradability. [23][24][25] The global market value of NMs will be expected to reach US$ 125 billion marks by 2024. [26] The widespread Nanomaterials (NMs) are widely used in commercial and medical products, such as cosmetics, vaccines, and drug carriers. Exposure to NMs via various routes such as dermal, inhalation, and ingestion has been shown to gain access to the systemic circulation, resulting in the accumulation of NMs in the liver. The unique organ structures and blood flow features facilitate...

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“…[30,66] However, despite these concerns there is currently no direct evidence for MoS 2 -induced hepatotoxicity except the documentation of pro-inflammatory effects (e.g., IL-1β, IL-6, and AIF gene) and occurrence of apoptosis in the liver tissue of zebrafish. [27] Additional experimentation in rodents is required for the further assessment of MoS 2 safety in vivo.…”

Section: Cations For Example Moomentioning

confidence: 99%

“…[25,26] For instance, Yu et al have demonstrated the development of localized liver lesions in adult zebrafish during exposure to dispersible MoS 2 micro-sheets. [27] However, the mechanism of liver toxicity at the molecular level is still unclear and there has been no attempt to differentiate between the adverse impact on specific liver cell types. This stresses the importance of understanding the differential effects of BN or MoS 2 on specific liver cell types that may encounter the 2D materials that are being delivered to liver sinusoids from hepatic and portal blood circulations.…”

Section: Introductionmentioning

confidence: 99%

Dissolution of 2D Molybdenum Disulfide Generates Differential Toxicity among Liver Cell Types Compared to Non‐Toxic 2D Boron Nitride Effects

Guiney

Downing

et al. 2021

Small

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2D boron nitride (BN) and molybdenum disulfide (MoS2) materials are increasingly being used for applications due to novel chemical, electronic, and optical properties. Although generally considered biocompatible, recent data have shown that BN and MoS2 could potentially be hazardous under some biological conditions, for example, during, biodistribution of drug carriers or imaging agents to the liver. However, the effects of these 2D materials on liver cells such as Kupffer cells (KCs), liver sinusoidal endothelial cells, and hepatocytes, are unknown. Here, the toxicity of BN and MoS2, dispersed in Pluronic F87 (designated BN‐PF and MoS2‐PF) is compared with aggregated forms of these materials (BN‐Agg and MoS2‐Agg) in liver cells. MoS2 induces dose‐dependent cytotoxicity in KCs, but not other cell types, while the BN derivatives are non‐toxic. The effect of MoS2 could be ascribed to nanosheet dissolution and the release of hexavalent Mo, capable of inducing mitochondrial reactive oxygen species generation and caspases 3/7‐mediated apoptosis in KUP5 cells. In addition, the phagocytosis of MoS2‐Agg triggers an independent response pathway involving lysosomal damage, NLRP3 inflammasome activation, caspase‐1 activation, IL‐1β, and IL‐18 production. These findings demonstrate the importance of Mo release and the state of dispersion of MoS2 in impacting KC viability.

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Dispersible MoS₂ micro-sheets induced a proinflammatory response and apoptosis in the gills and liver of adult zebrafish

Cited by 16 publications

References 45 publications

<p>Immunostimulatory Potential of MoS<sub>2</sub> Nanosheets: Enhancing Dendritic Cell Maturation, Migration and T Cell Elicitation</p>

<p>Immunostimulatory Potential of MoS<sub>2</sub> Nanosheets: Enhancing Dendritic Cell Maturation, Migration and T Cell Elicitation</p>

Understanding Nanomaterial–Liver Interactions to Facilitate the Development of Safer Nanoapplications

Dissolution of 2D Molybdenum Disulfide Generates Differential Toxicity among Liver Cell Types Compared to Non‐Toxic 2D Boron Nitride Effects

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