Disk Electrophoresis of Human Serum Lipoproteins

Narayan, K A; Narayan, Suhasini; Kummerow, Fred A.

doi:10.1038/205246a0

Cited by 104 publications

(15 citation statements)

References 7 publications

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“…Polyacrylamide gel disc electrophoresis of serum (100 L) was performed according to the method of Narayan et al 16 The pattern of electrophoresis was analyzed by densitometry.…”

Section: Polyacrylamide Gel Disc Electrophoresis Of Serum Lipoproteinsmentioning

confidence: 99%

Effect of MMP-2 Deficiency on Atherosclerotic Lesion Formation in ApoE-Deficient Mice

Kuzuya

Nakamura

Sasaki

et al. 2006

ATVB

193

123

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Objective-Although it has been reported that matrix metalloproteinase (MMP)-2 is a major proteinase in atherosclerotic plaque lesions, there is no direct evidence of the role of MMP-2 in atherosclerotic lesion formation. In the present study we determined the role of MMP-2 in atherosclerosis plaque development using apolipoprotein E-deficient (apoE Ϫ/Ϫ ) mice. Methods and Results-To generate MMP-2-deficient, apoE-deficient mice (MMP-2 Ϫ/Ϫ :apoE Ϫ/Ϫ ), MMP-2 Ϫ/Ϫ mice were crossed with apoE Ϫ/Ϫ mice. After 8 weeks of feeding with a lipid-rich diet, morphological and biochemical studies of the aortic sinus and arch were conducted. A significant reduction of the atherosclerotic plaque in the aortic sinus and arch with the decrease in smooth muscle cell-positive area was observed in MMP-2 Ϫ/Ϫ :apoE Ϫ/Ϫ mice compared with that of MMP-2 ϩ/ϩ :apoE Ϫ/Ϫ mice. Macrophage-and collagen-positive areas were less in aortic sinus but not in aortic arch in MMP-2 Ϫ/Ϫ :apoE Ϫ/Ϫ mice. There was no difference of MMP-9 mRNA expression in the plaque lesion between the 2 genotypes. A much lower level of mRNA expression of TIMP-1 and TIMP-2 was detected in the atherosclerotic plaque lesions of MMP-2 Ϫ/Ϫ :apoE Ϫ/Ϫ mice than in those of MMP-2 ϩ/ϩ :apoE Ϫ/Ϫ mice. Key Words: atherosclerosis Ⅲ collagen Ⅲ metalloproteinases Ⅲ plaque I n human or animal models of atherosclerosis, varying matrix metalloproteinases (MMPs) have been demonstrated to increase in atherosclerotic lesions, including MMP-1, -2, -3, -7, -9, -12, -13, and MT-MMPs. 1-3 MMPs have been believed to contribute to the development and progression of atherosclerosis. 1-3 However, there are only limited data providing direct evidence of the contribution of MMPs to the development of atherosclerotic lesions. Although MMP activity is commonly considered instrumental to the development of atherosclerotic lesions, this notion has been challenged by recent studies in gene-targeting mice. It has been reported that overexpression of the tissue inhibitor of metalloproteinases-1 (TIMP-1) reduced atherosclerotic lesions in apolipoprotein E-deficient (apoE Ϫ/Ϫ ) mice. 4 The deletion of the TIMP-1 gene resulted in either a reduction of or no change in the plaque size in the apoE Ϫ/Ϫ mice. 5,6 Atherosclerotic lesions were significantly larger in mice with a combined deficiency of apoE and MMP-3 than in apoE Ϫ/Ϫ mice. 7 Overexpression of MMP-1 in apoE Ϫ/Ϫ mice, which is not normally expressed in mice, decreased the extent of atherosclerosis. 8 A more recent study demonstrated that MMP-9 deficiency but not MMP-12 deficiency reduced the atherosclerotic lesion growth in apoE Ϫ/Ϫ mice. 9 MMP-13 deficiency had no effect on atherosclerotic plaque formation with similar accumulation of plaque macrophages and smooth muscle cells, but contained more interstitial collagen in apoE Ϫ/Ϫ mice. 10 These results seemingly contradict a central tenet in our understanding of atherosclerosis-that increased MMP activity leads to the formation of a thicker neointima-suggesting that the role of individual MMPs in pla...

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“…Polyacrylamide gel disc electrophoresis of serum (100 L) was performed according to the method of Narayan et al 16 The pattern of electrophoresis was analyzed by densitometry.…”

Section: Polyacrylamide Gel Disc Electrophoresis Of Serum Lipoproteinsmentioning

confidence: 99%

Effect of MMP-2 Deficiency on Atherosclerotic Lesion Formation in ApoE-Deficient Mice

Kuzuya

Nakamura

Sasaki

et al. 2006

ATVB

193

123

View full text Add to dashboard Cite

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“…Polyacrylamide gel was prepared in a 7 cm length x 5 mm internal diameter glass tube according to Narayan et al (1965 except that 6.75% polyacrylamide gel was used for the lower gel. An aliquot of serum was mixed with half the amount of ethylene glycol in which Sudan black B had been dissolved (McDonald and Ribeiro 1959).…”

Section: Methodsmentioning

confidence: 99%

“…and Narayan et al (1965 ) reported a rapid and simple electrophoresic method using polyacrylamide gel by which the amount of protein contained in the discrete subfractions was densitometrically quantitated. Hara et al (1980) showed that the high performance liquid chromatography made it possible to separate HDL into subfractions.…”

mentioning

confidence: 99%

A simple method for quantitative determination of high density lipoprotein 2- and 3-cholesterol concentrations by means of polyacrylamide gel electrophoresis.

Murai¹,

Miyahara²,

Tanaka

et al. 1982

Tohoku J. Exp. Med.

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“…Plasmaapolipoprotein concentrations weredeterminedby single radial immunodifusion methods. The plasma lipoprotein profile was analyzed by polyacrylamide gel electrophoresis (18). The separated lipoprotein fractions were stained with Sudan Black and detected with a densitometer.…”

Section: Case Reportmentioning

confidence: 99%

Clinical Characterization of a Case with Familial Hypobetalipoproteinemia Caused by Apo B-76, a New Truncation of Apolipoprotein B, Combined with Apo E2/E2 Phenotype.

et al. 2001

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Wereport a 43-year-old Japanese man with hypobetalipoproteinemia likely due to apolipoprotein (apo) B-76, a new truncation of apo B, and with homozygousity for the apo E2 isoform. He had no history suggestive of fat malabsorption and no sign of neurological disorder. His fasting baseline serum low-density lipoprotein (LDL) cholesterol and apo B levels were approximately half of normal. His plasma apo E level was elevated and its phenotype showed the E2/E2 homozygote. SDS-polyacrylamide gel electrophoresis of delipidated LDLfraction revealed a new truncated apo B, designated as apo B-76 according to the centile system of nomenclature. The postprandial lipid metabolism of the patient showedan almost normal response after fat loading. (Internal

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Disk Electrophoresis of Human Serum Lipoproteins

Cited by 104 publications

References 7 publications

Effect of MMP-2 Deficiency on Atherosclerotic Lesion Formation in ApoE-Deficient Mice

Effect of MMP-2 Deficiency on Atherosclerotic Lesion Formation in ApoE-Deficient Mice

A simple method for quantitative determination of high density lipoprotein 2- and 3-cholesterol concentrations by means of polyacrylamide gel electrophoresis.

Clinical Characterization of a Case with Familial Hypobetalipoproteinemia Caused by Apo B-76, a New Truncation of Apolipoprotein B, Combined with Apo E2/E2 Phenotype.

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