Disintegration of Cells by Extrusion Under Pressure

Edebo, L.

doi:10.1007/978-3-642-69148-5_10

Cited by 15 publications

(10 citation statements)

References 104 publications

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“…Preparations of somatic antigens (S) and the cell wall residue (W) were made from two strains of H. soninus (2336 and 939190). The frozen bacterial suspensions were homogenized by six pressings by an Xpress (AB Biox, Sweden) using a precooled (-30°C) 5 ml cylinder operated with a maximum force of 80 MPa (5). The product was centrifuged at 20,000 g at 5°C for I h, thereby separating the water soluble somatic antigens (S) and the cell wall residue (W) as the supernatant and the pellet, respectively.…”

Section: An I Igen Ic Prepuru T Ionsmentioning

confidence: 99%

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Development of a peroxidase‐antiperoxidase (PAP) technique for the identification of Haemophilus somnus in pneumonic calf lungs in Denmark

Tegtmeier¹,

Jensen²,

Jensen

1995

APMIS

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A peroxidase-antiperoxidase (PAP) technique was developed for the identification of Haemophilus somnus bacteria in lung tissues of calves. Antisera raised against somatic and wall antigens of a Danish and American strain of H. somnus were produced. Experimentally infected murine tissues were used for the determination of the sensitivity and specificity of antiserum that had been heterologously absorbed with antigens of cross-reacting bacteria, i.e. Pasteurella haemolytica and Pasteurella multocida. None of the antisera reacted with Actinomyces pyogenes. An antiserum raised against somatic antigens of the Danish strain of H. somnus revealed the highest sensitivity in the PAP technique and became specific following absorption. Heterologous absorption also rendered this antiserum specific in crossed immunoelectrophoresis. Subsequently, the PAP technique was applied on formalin-fixed pneumonic lung tissues of 86 calves. An immunodiagnosis of H. somnus pneumonia was obtained in 15 of 17 lungs from which the bacterium had been isolated. Moreover, immunostained bacteria were also demonstrated in 20 lungs from which H. somnus had not been isolated. Thus, application of immunohistochemistry significantly enhanced the diagnostic sensitivity of H. somnus pneumonia of calves and should be used as a potent supplementary tool for the routine screening of suspected lung tissues of calves from which bacterial isolation is negative.

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Section: An I Igen Ic Prepuru T Ionsmentioning

confidence: 99%

“…Antigenic preparations of P. haemolytica, P. multocida and A. pyogenes were produced and stored as described for H. somnus, but for the disruption of P. multocida and A. pyogenes a 25 ml cylinder operated at a maximum force of 200 MPa was used (5).…”

Section: An I Igen Ic Prepuru T Ionsmentioning

confidence: 99%

Development of a peroxidase‐antiperoxidase (PAP) technique for the identification of Haemophilus somnus in pneumonic calf lungs in Denmark

Tegtmeier¹,

Jensen²,

Jensen

1995

APMIS

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“…About 700 mg of mycelia were suspended in 10 mL of the extraction solution (50 mM Tris-HCl pH 8.0; 5 mM EDTA; 4 mM DTT; 2.5 M sucrose and 10 mg/mL bovine serum albumin) frozen at À20°C and disrupted under 28,000 psi in a chilled X-Press (Edebo, 1983). The thawed homogenate was centrifuged at 10,000g for 20 min and the supernatant was further centrifuged at 100,000g for 1 h. The 100,000g pellet was suspended in 2 mL of a solution containing 50 mM Tris-HCl pH 7.5, 4 mM DTT, 10 mg/mL bovine serum albumin and 2 M sucrose.…”

Section: Isolation Of Microsomesmentioning

confidence: 99%

Influence of electron transport proteins on the reactions catalyzed by Fusarium fujikuroi gibberellin monooxygenases

et al. 2008

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“…The frozen tissue blocks were disintegrated by being extruded twice in an X-press (AB Biox, Sweden) using a precooled (-30°C to -35°C) 25-ml cylinder operated at a maximal force of 200 Mpa (Edebo, 1983). The extruded material was centrifuged at 20 000g for 60 min at 5°C, and the supernatant was stored at -80°C until assayed.…”

Section: Disb2tegration Of Tissuesmentioning

confidence: 99%

Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle

Jensen

1993

Vet Res Commun

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A novel method for diagnosing bovine aspergillosis and zygomycosis is described. Rabbit hyperimmune antisera raised against somatic antigens of Aspergillus fumigatus and Absidia corymbifera were used in crossed immunoelectrophoresis with supernatants from disintegrated tissues from acute necrohaemorrhagic mycotic lesions from cattle. The method specifically identified 4 of 5 lesions with aspergillosis and 2 of 5 lesions with zygomycosis. One lesion dually infected with aspergillosis and zygomycosis was negative. The method worked with unabsorbed sera, was specific, and required only standard electrophoretic equipment. It can therefore supplement chemical detection of fungi in tissues in the diagnosis of bovine aspergillosis and zygomycosis.

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Disintegration of Cells by Extrusion Under Pressure

Cited by 15 publications

References 104 publications

Development of a peroxidase‐antiperoxidase (PAP) technique for the identification of Haemophilus somnus in pneumonic calf lungs in Denmark

Development of a peroxidase‐antiperoxidase (PAP) technique for the identification of Haemophilus somnus in pneumonic calf lungs in Denmark

Influence of electron transport proteins on the reactions catalyzed by Fusarium fujikuroi gibberellin monooxygenases

Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle

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