Influence of electron transport proteins on the reactions catalyzed by Fusarium fujikuroi gibberellin monooxygenases

Cárcamo-Vega, José J.; Hedden, Peter; Tudzynski, Bettina; Rojas, María Cecilia

doi:10.1016/j.phytochem.2007.08.026

Cited by 19 publications

(13 citation statements)

References 45 publications

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“…Purified fractions containing 14 C-labeled products were derivatized with ethereal diazomethane and N -methyl- N -trimethylsilyltrifluoroacetamide and identified by GC-MS as described 60 . Compounds were identified by comparison of their mass spectra with published spectra 57 .…”

Section: Methodsmentioning

confidence: 99%

Elucidation of gibberellin biosynthesis in bacteria reveals convergent evolution

et al. 2016

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Gibberellins (GAs) are crucial phytohormones involved in many aspects of plant growth and development, including plant-microbe interactions, which has led to GA production by plant-associated fungi and bacteria as well. While the GA biosynthetic pathways in plants and fungi have been elucidated and found to have independently arisen through convergent evolution, little has been uncovered about GA biosynthesis in bacteria. Some nitrogen-fixing/symbiotic, legume-associated rhizobia, including Bradyrhizobium japonicum, the symbiont of soybean, and Sinorhizobium fredii, a broad-host-nodulating species, contain a putative GA biosynthetic operon/gene cluster. Through functional characterization of five unknown genes, we demonstrate that this operon encodes the enzymes necessary to produce GA9, thereby elucidating bacterial GA biosynthesis. The distinct nature of these enzymes indicates that bacteria have independently evolved a third biosynthetic pathway for GA production. Furthermore, our results also reveal a central biochemical logic that is followed in all three convergently evolved GA biosynthetic pathways.

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Section: Methodsmentioning

confidence: 99%

Elucidation of gibberellin biosynthesis in bacteria reveals convergent evolution

et al. 2016

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“…This may indicate that these fungal intermediates do not leave the active site, and their presence in fungal cultures may be due to hydrolysis of enzyme-bound intermediates [41]. However, cultures of F. fujikuroi grown in the presence of 18 O 2 or H 2 18 O incorporated 18 O only from 18 O 2 into the intermediates, indicating that they were not formed by hydrolysis [60].…”

Section: The Ga Biosynthetic Pathwaymentioning

confidence: 99%

Gibberellin biosynthesis and its regulation

Hedden

Thomas

2012

Biochemical Journal

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The GAs (gibberellins) comprise a large group of diterpenoid carboxylic acids that are ubiquitous in higher plants, in which certain members function as endogenous growth regulators, promoting organ expansion and developmental changes. These compounds are also produced by some species of lower plants, fungi and bacteria, although, in contrast to higher plants, the function of GAs in these organisms has only recently been investigated and is still unclear. In higher plants, GAs are synthesized by the action of terpene cyclases, cytochrome P450 mono-oxygenases and 2-oxoglutarate-dependent dioxygenases localized, respectively, in plastids, the endomembrane system and the cytosol. The concentration of biologically active GAs at their sites of action is tightly regulated and is moderated by numerous developmental and environmental cues. Recent research has focused on regulatory mechanisms, acting primarily on expression of the genes that encode the dioxygenases involved in biosynthesis and deactivation. The present review discusses the current state of knowledge on GA metabolism with particular emphasis on regulation, including the complex mechanisms for the maintenance of GA homoeostasis.

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“…The 14 C-labelled products eluted from HPLC were derivatized as methyl esters (ethereal diazomethane), TMSi ethers (N-methyl-Ntrimethylsilyltrifluoroacetamide) and identified by GC-MS as described (Troncoso et al, 2008). Compounds were identified by comparison of their mass spectra with those of authentic samples and/or with published spectra (Gaskin and MacMillan, 1992).…”

Section: Product Identificationmentioning

confidence: 99%

Gibberellin biosynthesis and gibberellin oxidase activities in Fusarium sacchari, Fusarium konzum and Fusarium subglutinans strains

et al. 2010

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Influence of electron transport proteins on the reactions catalyzed by Fusarium fujikuroi gibberellin monooxygenases

Cited by 19 publications

References 45 publications

Elucidation of gibberellin biosynthesis in bacteria reveals convergent evolution

Elucidation of gibberellin biosynthesis in bacteria reveals convergent evolution

Gibberellin biosynthesis and its regulation

Gibberellin biosynthesis and gibberellin oxidase activities in Fusarium sacchari, Fusarium konzum and Fusarium subglutinans strains

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