Disinfection of Oreochromis mossambicus (Peters) eggs against commonly occurring potentially pathogenic bacteria and fungi under artificial hatchery conditions

Subasinghe, R. P.; Sommerville, Christina

doi:10.1111/j.1365-2109.1985.tb00301.x

Cited by 19 publications

(20 citation statements)

References 10 publications

(17 reference statements)

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“…were also reported in Mozambique tilapia (O. mossambicus) eggs from the artificial incubation systems (Subasinghe and Sommerville 1985). In our study, A. hydrophila was the most frequently found isolate.…”

Section: Discussionsupporting

confidence: 49%

“…Treasurer et al (2005) reported the number of 3100 to 38 000 CFU/ml of egg in cod and haddock, while the colony counts in California yellowtail, white seabass, and California halibut were 16.7, 110.0, and 233.3 CFU/ml of egg, respectively (Stuart et al 2010). In our study, the bacterial counts (1.57 × 10 3 CFU/ml) in the hatchery water were comparable to those in the rearing system of California halibut (10 4 CFU/ml) (Stuart et al 2010) and of Mozambique tilapia (6.25 × 10 3 CFU/ml) (Subasinghe and Sommerville 1985). The relatively high numbers of bacteria colonized on the surface of Nile tilapia eggs may be attributed to the rapid growth of the waterborne pathogens in temperate water temperature, particularly in the hatchery facility that is in the tropical region as Thailand, and the gelatinous matrix surrounding the eggs may be an appropriate substrate for bacterial proliferation.…”

Section: Discussionmentioning

confidence: 49%

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Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticus

Jantrakajorn¹,

Wongtavatchai²

2015

Czech J. Anim. Sci.

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ABSTRACT:Intensive tilapia egg incubation techniques create favourable conditions for microbial proliferation which often leads to massive mortalities of fish larvae. The effects of Nile tilapia (Oreochromis niloticus) eggs exposure to bronopol on decontamination of their bacterial surface and survival of larvae were observed. Immersion treatments of fertilized eggs were applied at 10, 25, 50, 100, and 250 mg/l of bronopol for 10, 20, and 30 min. This treatment substantially reduced the number of bacteria on Nile tilapia eggs. The greatest reduction in bacterial numbers (1.58 × 10 3 colony forming units/g of egg) was observed at the maximum treatment dosage, i.e. 250 mg/l for 30 min, but this was not significant when compared with treatments of 100-250 mg/l bronopol for 10-30 min. Treatments of 50-250 mg/l bronopol provided better larval survival (89.33-94.67%) than those of < 50 mg/l (P < 0.05). The larval survival obtained from the Nile tilapia eggs disinfected with 10 and 25 mg/l bronopol for 10-30 min was similar to that of negative controls (79.33-80.97%). Additional in vitro test was performed to determine the inhibitory potency of bronopol against bacteria cultured from fertilized Nile tilapia eggs. Twenty-two bacterial isolates (Aeromonas hydrophila (n = 12), Staphylococcus aureus (n = 6), Escherichia coli (n = 2), and Micrococcus spp. (n = 2)) responded to minimum inhibitory concentrations of bronopol ranging from 64 to 128 µg/ml. The study demonstrates that the immersion treatment of Nile tilapia fertilized eggs with 250 mg/l for 10-30 min, the concentration proven effective against bacteria cultured from fertilized eggs, significantly reduced bacterial load and improved larval survival.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 49%

Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticus

Jantrakajorn¹,

Wongtavatchai²

2015

Czech J. Anim. Sci.

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“…In vitro studies have shown that a 5 min treatment with 25 mg active iodine 1-l is effective against several fish pathogens (Amend and Pie&h, 1972;Ross and Smith, 1972) and lO-15min treatments with 50-100 mg active iodine 1-l have been recommended for surface disinfection of fis,h eggs from many freshwater species (McFadden, 1969;Amend, 1974;Schachte, 1979;Subasinghe and Sommerville, 1985). Positive effects in seawater of treating Atlantic halibut eggs for 10 min with 50 mg active iodine 1-l have also been reported (Bergh et al, 1991).…”

Section: Discussionmentioning

confidence: 96%

“…At higher doses of Buffodine the bactericidal effect was better, but these doses did not have a good safety margin to lethal doses. Toxic effects have previously been observed after 10-15 min treatments with 2 200 mg active iodine 1-l (McFadden, 1969;Wright and Snow, 1975;Subasinghe and Sommerville, 1985). The toxicity of iodophores is pH dependent, i.e.…”

Section: Discussionmentioning

confidence: 98%

“…In most freshwater studies, the use of aldehydes as a surface disinfectant for fish eggs has not been promising (Gee and Sarles, 1942;Cline and Post, 1972;Wright and Snow, 1975;Subasinghe and Sommerville, 1985). Surface sterile eggs of Tilapia macrocephafa have, however, been obtained after 10 min treatment with a 0.04% solution of formaldehyde, and larvae from treated eggs survived under sterile conditions until they eventually died of starvation (Shaw, 1957).…”

Section: Discussionmentioning

confidence: 99%

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Surface disinfection of eggs from marine fish: evaluation of four chemicals

Salvesen

Vadstein

1995

Aquacult Int

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For surface disinfection of marine fish eggs Buffodine (1.06% free iodine), glutaraldehyde, chloramine-T and sodium hypochlorite (5% free chlorine) were tested using plaice (Pleuronectes plutessa L.) as the main species for evaluation. Glutaraldehyde was the most promising candidate of the four chemicals tested. Good bactericidal effects without any documented negative effects on eggs and larvae were obtained at concentrations of 400-600 mg 1-l and contact times of 5-10 min. Replicated experiments under identical disinfection conditions revealed a clear correlation between the degree of successful surface disinfection and the initial bacterial load of the egg batch.

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Chemical treatment of lobster eggs against epibiotic bacteria

1996

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Eggs of the European lobster, Homarus gammarus (L.), were exposed to malachite green (5, 10, 15 mg 1-1:10 min), glutaraldehyde (50, 100, 150 mg 1-1:3 min) and iodine as Buffodine TM (50, 100, 150 mg 1-~: 10 min). The efficiency of the treatments was tested by incubating eggs individually in wells of multiwell dishes with TSB agar for 14 days after exposure. In order to find any effect on viability, batches of 30 eggs from each of three females were incubated artificially in a recirculation system for 19 days and repeatedly exposed to the disinfectants. Iodine as Buffodine (150 mg 1-1) was the only treatment that resulted in a significant decrease of the bacterial growth on lobster eggs, but the treatment also resulted in inhibited hatching compared with the control group. Thus, our results indicate that treatment with 150mg-1 iodine as Buffodine could be a strategy for reducing bacterial growth on lobster eggs when massive egg mortality due to bacteria is otherwise unavoidable. The treatment could, however, lead to decreased viability of larvae due to inhibited hatching.

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Disinfection of Oreochromis mossambicus (Peters) eggs against commonly occurring potentially pathogenic bacteria and fungi under artificial hatchery conditions

Cited by 19 publications

References 10 publications

Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticus

Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticus

Surface disinfection of eggs from marine fish: evaluation of four chemicals

Chemical treatment of lobster eggs against epibiotic bacteria

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