Surface disinfection of eggs from marine fish: evaluation of four chemicals

Salvesen, I.; Vadstein, Ôlav

doi:10.1007/bf00118098

Cited by 85 publications

(64 citation statements)

References 24 publications

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“…Se ha probado con éxito a altas concentraciones (hasta 1600 mg L -1 ) en diversas especies principalmente de aguas frías como la platija (Pleuronectes platessa), bacalao (Gadhus morhua), lenguado del Atlántico (Hippoglossus hippoglossus) (Salvesen & Vadstein 1995) y en especies de la familia Sparide como el sargo (Diplodus sargo), besugo (Pagrus pagrus) y la dorada (Sparus aurata) (Escaffre et al 2001, Katharios et al 2007). La selección de las dosis del glutaraldehído en el presente estudio, fue en base al trabajo realizado por Ibarra-Castro (2008), quien lo evaluó en huevos del pargo lunarejo (Lutjanus guttatus), y obtuvo resultados de eclosión menores a 80%.…”

Section: Discussionunclassified

“…La acriflavina se ha usado tradicionalmente para desinfectar huevos de peces marinos como es el caso del barramundi (Lates calcarifer), obteniendo resultados de eclosión mayores de 80% (Tucker 1998, Álvarez-Lajonchère & Tsuzuki 2008. El uso de la formalina para desinfectar huevos de especies dulceacuícolas y marinas ha sido muy eficiente, además es un agente químico aprobado en Estados Unidos de América para el mantenimiento saludable de cultivos intensivos de peces (Van Waters 1988, Shereier et al 1996, Gieseker et al 2006, Ibarra-Castro 2008; así mismo, el glutaraldehído se ha aplicado en especies marinas debido a su amplio espectro de actividad para desinfectar los huevos (Block 1977, Vadstein et al 1993, Harboe et al 1994, Salvesen & Vadstein 1995.…”

Section: Introductionunclassified

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Efecto de la acriflavina, formalina y glutaraldehído sobre la desinfección y la eclosión de los huevos del botete diana Sphoeroides annulatus

Rodríguez-Ibarra

Parra

et al. 2011

Rev. biol. mar. oceanogr.

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Section: Discussionunclassified

Section: Introductionunclassified

Efecto de la acriflavina, formalina y glutaraldehído sobre la desinfección y la eclosión de los huevos del botete diana Sphoeroides annulatus

Rodríguez-Ibarra

Parra

et al. 2011

Rev. biol. mar. oceanogr.

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“…For adequate egg disinfection, chemicals other than iodine may be necessary, such as glutaraldehyde (Salvesen & Vadstein 1995, Salvesen et al 1997. For treatment of largemouth bass Micropterus salmoides eggs, acriflavine (500 to 700 ppm for 15 min) was the disinfectant recommended over 5 other disinfectants by Wright & Snow (1975).…”

Section: Discussionmentioning

confidence: 99%

Physical and chemical effects on viability of the Myxobolus cerebralis triactinomyxon

Wagner¹,

Smith²,

Arndt³

et al. 2003

Dis. Aquat. Org.

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Various chemical and physical methods for destroying the triactinomyxon (TAM) stage of the myxozoan parasite Myxobolus cerebralis were tested. The fluorescent stains propidium iodide and fluorescein diacetate were used as indicators of viability. Physical variables tested included freezing, drying, high temperature, sonication, and pressure of 6.2 × 10 7 Pa (9000 psi). Chemicals evaluated included chlorine bleach, povidone-iodine, and hydrogen peroxide. Freezing or drying for 1 h was effective in killing TAMs, but pressure was not. Temperatures above 75°C for at least 5 min were also effective. Sonication with a laboratory instrument cleaner for 10 to 13 min killed and ruptured TAMs, resulting in <1.9% recovery. However, among the surviving TAMs, 39 to 58% were still viable. Chlorine concentrations of 130 ppm for 10 min were also effective at temperatures ranging from ice-water to room temperature and total hardness ranging from 10 to 500 mg l -1. Lethal concentrations of hydrogen peroxide and povidone-iodine (10% solution) were quite high: 10% for 10 min, and 50% (5000 ppm active iodine) for 60 min, respectively. The stain results indicating TAM death were verified in 2 tests in which rainbow trout Oncorhynchus mykiss were exposed to TAMs that had been either frozen for 1 h or treated with 66 ppm chlorine as sodium hypochlorite for 1 min. None of the fish exposed to the treated TAMs became infected. These results should provide disinfection guidelines to prevent transfer of M. cerebralis TAMs to uninfected areas and provide information on the risks of parasite transfer under various treatment scenarios. KEY WORDS: Myxobolus · Whirling disease · Disinfection · Chlorine · UltrasoundResale or republication not permitted without written consent of the publisher

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“…A wide range of chemicals were reported as egg disinfectants for the routine use in fish hatcheries, e.g. benzalkonium chloride (Douillet and Holt 1994), glutaraldehyde (Salvesen and Vadstein 1995), formalin (Stuart et al 2010), hydrogen peroxide (Rach et al 1998;Rasowo et al 2007), iodine compounds (Salvesen and Vadstein 1995;El-Dakour et al 2013), and chlorine-based disinfectants such as sodium hypochlorite (Harboe et al 1994) and bronopol (Birkbeck et al 2006;Oono et al 2007). These disinfectants proved to be safe for different species of fish eggs, while highly toxic to aquatic pathogens.…”

Section: Introductionmentioning

confidence: 99%

Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticus

Jantrakajorn¹,

Wongtavatchai²

2015

Czech J. Anim. Sci.

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ABSTRACT:Intensive tilapia egg incubation techniques create favourable conditions for microbial proliferation which often leads to massive mortalities of fish larvae. The effects of Nile tilapia (Oreochromis niloticus) eggs exposure to bronopol on decontamination of their bacterial surface and survival of larvae were observed. Immersion treatments of fertilized eggs were applied at 10, 25, 50, 100, and 250 mg/l of bronopol for 10, 20, and 30 min. This treatment substantially reduced the number of bacteria on Nile tilapia eggs. The greatest reduction in bacterial numbers (1.58 × 10 3 colony forming units/g of egg) was observed at the maximum treatment dosage, i.e. 250 mg/l for 30 min, but this was not significant when compared with treatments of 100-250 mg/l bronopol for 10-30 min. Treatments of 50-250 mg/l bronopol provided better larval survival (89.33-94.67%) than those of < 50 mg/l (P < 0.05). The larval survival obtained from the Nile tilapia eggs disinfected with 10 and 25 mg/l bronopol for 10-30 min was similar to that of negative controls (79.33-80.97%). Additional in vitro test was performed to determine the inhibitory potency of bronopol against bacteria cultured from fertilized Nile tilapia eggs. Twenty-two bacterial isolates (Aeromonas hydrophila (n = 12), Staphylococcus aureus (n = 6), Escherichia coli (n = 2), and Micrococcus spp. (n = 2)) responded to minimum inhibitory concentrations of bronopol ranging from 64 to 128 µg/ml. The study demonstrates that the immersion treatment of Nile tilapia fertilized eggs with 250 mg/l for 10-30 min, the concentration proven effective against bacteria cultured from fertilized eggs, significantly reduced bacterial load and improved larval survival.

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Surface disinfection of eggs from marine fish: evaluation of four chemicals

Cited by 85 publications

References 24 publications

Efecto de la acriflavina, formalina y glutaraldehído sobre la desinfección y la eclosión de los huevos del botete diana Sphoeroides annulatus

Efecto de la acriflavina, formalina y glutaraldehído sobre la desinfección y la eclosión de los huevos del botete diana Sphoeroides annulatus

Physical and chemical effects on viability of the Myxobolus cerebralis triactinomyxon

Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticus

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