Disinfection efficacy over yeast biofilms of juice processing industries

Abstract

Membrane separation systems represent a hot - spot for biofilm formation in juice industries. Sodium hypochlorite (NaOCl) has been traditionally the disinfectant of choice; however, its effectiveness over well-established biofilms is limited. In this work the study of biofilm formation on ultrafiltration membranes was proposed. The effectiveness of cleaning and disinfection procedures commonly used in juice industry was tested on the removal and killing of cells. The species used (Rhodotorula mucilaginosa, Candida krusei, Candida kefyr and Candida tropicalis) were isolated from ultrafiltration modules of a clarified apple juice industry. Industrial concentrations of NaOCl (200mgCL∙L) showed to be effective against planktonic cultures with >4 log reductions, whereas their overall efficiency against adhered cells was smaller. Recovery of viable cell counts to initial numbers was evidenced regardless of the time of colonization. The topography of the surface showed to have an impact on the efficiency of the disinfectant, presenting membranes smaller log reductions than stainless steel (~1.09-1.53logCFU). At 200mgCl∙L only membrane's cross flow recovery was reached with no long-term effect over the attached cells. The overall results demonstrated the recalcitrance of these biofilms to typical cleaning and disinfection process which may confer them with a selective advantage.

Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes

“…are among the most common yeast species associated with UF membranes in fruit juice processing plants (Tarifa et al . ). Candida tropicalis L5, a strain previously isolated from UF membranes, strongly adheres to the surface of stainless steel, forming resistant biofilms which may confer a competitive advantage over other yeasts (Brugnoni et al .…”

“…Candida spp. are among the most common yeast species associated with UF membranes in fruit juice processing plants (Tarifa et al 2018). Candida tropicalis L5, a strain previously…”

Monitoring Growth and Removal of Pseudomonas Biofilms on Cellulose-Based Fabrics

“…Since microorganisms residing on textiles can have several harmful effects, it is important to avoid microbial growth or to remove established biofilms from the material. Many conventional mechanical and chemical routine methods are available to contain biofilm formation in different industrial equipment [58], but these methods do not work for every setting where reliable removal is needed, such as for biofilm removal from various types of fabrics. Therefore, in this study, once it was observed that Pseudomonas species adhere strongly to the cellulose surface, different washing formulations (based on detergent and enzymatic solutions) were tested to remove biofilms of Pseudomonas spp.…”

Technologies and Extraction Methods of Polyphenolic Compounds Derived from Pomegranate (Punica granatum) Peels. A Mini Review

“…Pomegranate juice wastes are the result of the fruits squeezing process. Due to the large amounts of wastes produced in the fruit juice industry every cultivation period, this industrial activity is of social [13], economic [14,15], and ecological importance [16,17].…”