Diseases of dab (Limanda limanda): Analysis and assessment of data on externally visible diseases, macroscopic liver neoplasms and liver histopathology in the North Sea, Baltic Sea and off Iceland

Lang, Thomas; Feist, Stephen W.; Stentiford, Grant D.; Bignell, John P.; Vethaak, A.D.; Wosniok, Werner

doi:10.1016/j.marenvres.2015.12.009

Cited by 25 publications

(19 citation statements)

References 20 publications

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“…A Fish Disease Index based on externally visible diseases and parasites, visible liver neoplasms and other histopathologically detected liver lesions has been developed and used extensively in the North Sea, Baltic Sea, and off Iceland. This index was found to be an important tool as an ecosystem health indicator 52 .…”

Section: Discussionmentioning

confidence: 98%

Necropsy-based Wild Fish Health Assessment

Blazer

Walsh

Braham

et al. 2018

JoVE

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Anthropogenic influences from increased nutrients and chemical contaminants, to habitat alterations and climate change, can have significant effects on fish populations. Adverse effects monitoring, utilizing biomarkers from the organismal to the molecular level, can be used to assess the cumulative effects on fishes and other organisms. Fish health has been used worldwide as an indicator of aquatic ecosystem health. The necropsy-based fish health assessment provides data on visible abnormalities and lesions, parasites, condition and organosomatic indices. These can be compared by site, season and sex, as well as temporally, to document change over time. Severity ratings can be assigned to various observations to calculate a fish health index for more quantitative assessment. A drawback of the necropsy-based assessment is that it is based on visual observations and condition factors, which are not as sensitive as tissue and subcellular biomarkers for sublethal effects. Additionally, it is rarely possible to identify causes or risk factors associated with observed abnormalities. So, for instance a raised lesion or "tumor" on the fins, lips or body surface may be a neoplasm. However, it could also be a response to a parasite, chronic inflammation or hyperplasia of normal cells in response to an irritant. Conversely, neoplasms, certain parasites, other infectious agents and many tissue changes are not visible and so may be underestimated. However, during the necropsy-based assessment, blood (plasma), tissues for histopathology (microscopic pathology), genomics and other molecular analyses, and otoliths for aging can be collected. These downstream analyses, together with geospatial analyses, habitat assessments, water quality and contaminant analyses can all be important in comprehensive ecosystem evaluations.

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Section: Discussionmentioning

confidence: 98%

Necropsy-based Wild Fish Health Assessment

Blazer

Walsh

Braham

et al. 2018

JoVE

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“…Despite their importance, these skin lesions were previously merely scored macroscopically using a crude semi‐quantitative scoring system of the skin ulcerations based on their presence, intensity and impact on the host. The scoring of skin ulcerations is part of the calculated Fish Disease Index (FDI) used as indicators for the health of the marine environment (Bucke et al., ; Lang et al., ). This superficial scoring was overcome in the present study, that is, by sampling freshly killed fish on board of the research vessel and perform a thorough sampling to avoid the confounding effects of post‐mortem degeneration, bacterial overgrowth (Khuntia, ) and generating a broad database.…”

Section: Discussionmentioning

confidence: 99%

“…Despite their importance, these skin lesions were previously merely scored macroscopically using a crude semi-quantitative scoring system of the skin ulcerations based on their presence, intensity and impact on the host. The scoring of skin ulcerations is part of the calculated Fish Disease Index (FDI) used as indicators for the health of the marine environment (Bucke et al, 1996;Lang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

First isolation of Vibrio tapetis and an atypical strain of Aeromonas salmonicida from skin ulcerations in common dab (Limanda limanda) in the North Sea

Vercauteren

Swaef

Declercq

et al. 2017

Journal of Fish Diseases

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Skin ulcerations rank amongst the most prevalent lesions affecting wild common dab (Limanda limanda) with an increase in prevalence of up to 3.5% in the Belgian part of the North Sea. A complex aetiology of these ulcerations is suspected, and many questions remain on the exact factors contributing to these lesions. To construct the aetiological spectrum of skin ulcerations in flatfish, a one-day monitoring campaign was undertaken in the North Sea. Fifteen fish presented with one or more ulcerations on the pigmented and/or non-pigmented side. Pathological features revealed various stages of ulcerations with loss of epidermal and dermal tissue, inflammatory infiltrates and degeneration of the myofibers bordering the ulceration, albeit in varying degrees. Upon bacteriological examination, pure cultures of Vibrio tapetis were retrieved in high numbers from five fish and of Aeromonas salmonicida in one fish. The V. tapetis isolates showed cross-reactivity with the sera against the representative strain of serotype O2 originating form a carpet-shell clam (Ruditapes descussatus). Moreover, the A. salmonicida isolates displayed a previously undescribed vapA gene sequence (A-layer type) with possible specificity towards common dab. Further research is necessary to pinpoint the exact role of these agents in the development of skin ulcerations in common dab.

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“…The approach presented here adds to our understanding of the mechanisms of liver tumors in sentinel marine flatfish. This greater insight into the drivers of disease will further aid in the interpretation of field data where fish are increasingly beings used as sentinels of wider environmental health (Stentiford et al, 2010;Lang et al, 2015;Lyons et al, 2015). CDKN1B with mean data plotted (+SD); n=12 fish for normal and n=8 fish for HCA.…”

Section: Mrna Transcripts Down-regulated In Dab Liver Adenoma Cells Rmentioning

confidence: 99%

Hepatocellular adenoma in a European flatfish (Limanda limanda): Genetic alterations in laser-capture micro-dissected tissue and global transcriptomic approach

Lerebours

Chapman

Lyons

et al. 2017

Marine Pollution Bulletin

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Liver tumours in flatfish have been diagnosed using histopathology for decades to monitor the impacts of marine pollution. Here we describe the application of specific gene (retinoblastoma, Rb) profiling in laser capture micro-dissected samples, and a suppression subtractive hybridization (SSH) approach to isolate differentially expressed genes in hepatocellular adenoma (HCA) samples from dab, Limanda limanda. The Rb profiles from apparently normal and HCA micro-dissected samples of fish from the North Sea showed no significant difference, and genotypic heterogeneity within defined histological phenotypes was observed. In the SSH, sequences associated with cell signalling, cell cycle, gene expression regulation, protein transport and protein degradation were isolated. These included up-regulation of arrestin domain containing 3 (arrdc3), Rac-1 and tribbles, and down-regulation of ankyrin repeat/sterile alpha-motif domain-containing protein 1B-like (ANKS1B-like), c-fos, CDKN1B and RhoA-like sequences, previously implicated in mammalian HCA. This study offers new candidates involved in fish liver tumour development.

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Diseases of dab (Limanda limanda): Analysis and assessment of data on externally visible diseases, macroscopic liver neoplasms and liver histopathology in the North Sea, Baltic Sea and off Iceland

Cited by 25 publications

References 20 publications

Necropsy-based Wild Fish Health Assessment

Necropsy-based Wild Fish Health Assessment

First isolation of Vibrio tapetis and an atypical strain of Aeromonas salmonicida from skin ulcerations in common dab (Limanda limanda) in the North Sea

Hepatocellular adenoma in a European flatfish (Limanda limanda): Genetic alterations in laser-capture micro-dissected tissue and global transcriptomic approach

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