Disease Status and Yield Losses Due to Wet Bubble Disease (Mycogone perniciosa) Associated with the Cultivation of White Button Mushroom at Different Mushroom Units of Kashmir Valley

Kouser, Shaheen; Ahmed, Mohammad Bulbul; Shah, Shaiesta

doi:10.3923/ppj.2013.104.109

Cited by 8 publications

(2 citation statements)

References 5 publications

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“…Production of button mushrooms in China has rapidly increased in recent years as a result of the expanded area of cultivation and the adoption of technology for improved commercial cultivation (He et al, 2014;Sonnenberg et al, 2017;Li et al, 2018;McGee, 2018). However, diseases caused by fungi, bacteria, and viruses are major constraints to A. bisporus production worldwide, often leading to serious economic losses (Fletcher et al, 1989;Largeteau and Savoie, 2010;Kouser et al, 2013). Wet bubble disease (WBD) is one of the most devastating diseases of A. bisporus, causing yield losses of 15-30% under favorable conditions and up to 75% or total crop loss in the most severe cases (Zhou et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of Chitinase GH18 Gene Family in Mycogone perniciosa

Yang

Sossah²,

Li³

et al. 2021

Front. Microbiol.

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Mycogone perniciosa causes wet bubble disease in Agaricus bisporus and various Agaricomycetes species. In a previous work, we identified 41 GH18 chitinase genes and other pathogenicity-related genes in the genome of M. perniciosa Hp10. Chitinases are enzymes that degrade chitin, and they have diverse functions in nutrition, morphogenesis, and pathogenesis. However, these important genes in M. perniciosa have not been fully characterized, and their functions remain unclear. Here, we performed a genome-wide analysis of M. perniciosa GH18 genes and analyzed the transcriptome profiles and GH18 expression patterns in M. perniciosa during the time course of infection in A. bisporus. Phylogenetic analysis of the 41 GH18 genes with those of 15 other species showed that the genes were clustered into three groups and eight subgroups based on their conserved domains. The GH18 genes clustered in the same group shared different gene structures but had the same protein motifs. All GH18 genes were localized in different organelles, were unevenly distributed on 11 contigs, and had orthologs in the other 13 species. Twelve duplication events were identified, and these had undergone both positive and purifying selection. The transcriptome analyses revealed that numerous genes, including transporters, cell wall degrading enzymes (CWDEs), cytochrome P450, pathogenicity-related genes, secondary metabolites, and transcription factors, were significantly upregulated at different stages of M. perniciosa Hp10 infection of A. bisporus. Twenty-three out of the 41 GH18 genes were differentially expressed. The expression patterns of the 23 GH18 genes were different and were significantly expressed from 3 days post-inoculation of M. perniciosa Hp10 in A. bisporus. Five differentially expressed GH18 genes were selected for RT-PCR and gene cloning to verify RNA-seq data accuracy. The results showed that those genes were successively expressed in different infection stages, consistent with the previous sequencing results. Our study provides a comprehensive analysis of pathogenicity-related and GH18 chitinase genes’ influence on M. perniciosa mycoparasitism of A. bisporus. Our findings may serve as a basis for further studies of M. perniciosa mycoparasitism, and the results have potential value for improving resistance in A. bisporus and developing efficient disease-management strategies to mitigate wet bubble disease.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of Chitinase GH18 Gene Family in Mycogone perniciosa

Yang

Sossah²,

Li³

et al. 2021

Front. Microbiol.

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“…Mushrooms contain compounds such as minerals, vitamins, proteins and polysaccharides, and are therefore considered as nutritious foods [1] [2]. Commercial mushroom production is around 16 million tons annually worldwide and more than 75% of these mushrooms are produced in China, where the total value of mushroom production is ranked the fifth in terms of crop value [3].…”

Section: Introductionmentioning

confidence: 99%

The <i>PafR</i> Gene Is Required for Antifungal Activity of Strain MS82 against <i>Mycogone perniciosa</i>

Ma¹,

Wang²,

Deng³

et al. 2017

AiM

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Bacterial strain MS82, isolated from the rhizosphere of a soybean plant, belongs to the species Pseudomonas fluorescens. The most important feature of strain MS82 is the production of antifungal activity against the mushroom pathogenic fungus Mycogone perniciosa but not against the mushroom fungus Agaricus bisporus. In this study, the mutant MS82MT19 generated with the EZ-Tn5 transposon system completely lost the antifungal activity against M.perniciosa. An open-reading frame named as PafR and predicted to code for a sensory box GGDEF/EAL domain protein, was disrupted in MS82MT19. To further confirm the function of this gene, site-directed mutagenesis with insertion of the terminatorless nptII cassette into the PafR gene was used to generate the mutant MS82SD19. As expected, there was no detectable antifungal activity of mutant MS82SD19 against M. perniciosa. These results suggest that the PafR gene plays an important role in the production of antifungal activity of P. fluorescens strain MS82.

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De novo genome sequencing of mycoparasite Mycogone perniciosa strain MgR1 sheds new light on its biological complexity

Kumar¹,

Sharma²,

Kumar³

et al. 2021

Braz J Microbiol

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Disease Status and Yield Losses Due to Wet Bubble Disease (Mycogone perniciosa) Associated with the Cultivation of White Button Mushroom at Different Mushroom Units of Kashmir Valley

Cited by 8 publications

References 5 publications

Genome-Wide Identification and Analysis of Chitinase GH18 Gene Family in Mycogone perniciosa

Genome-Wide Identification and Analysis of Chitinase GH18 Gene Family in Mycogone perniciosa

The <i>PafR</i> Gene Is Required for Antifungal Activity of Strain MS82 against <i>Mycogone perniciosa</i>

De novo genome sequencing of mycoparasite Mycogone perniciosa strain MgR1 sheds new light on its biological complexity

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Disease Status and Yield Losses Due to Wet Bubble Disease (Mycogone perniciosa) Associated with the Cultivation of White Button Mushroom at Different Mushroom Units of Kashmir Valley

Cited by 8 publications

References 5 publications

Genome-Wide Identification and Analysis of Chitinase GH18 Gene Family in Mycogone perniciosa

Genome-Wide Identification and Analysis of Chitinase GH18 Gene Family in Mycogone perniciosa

The &lt;i&gt;PafR&lt;/i&gt; Gene Is Required for Antifungal Activity of Strain MS82 against &lt;i&gt;Mycogone perniciosa&lt;/i&gt;

De novo genome sequencing of mycoparasite Mycogone perniciosa strain MgR1 sheds new light on its biological complexity

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The <i>PafR</i> Gene Is Required for Antifungal Activity of Strain MS82 against <i>Mycogone perniciosa</i>