In recent years, metagenomics has emerged as a powerful tool for mining of hidden microbial treasure in a culture independent manner. In the last two decades, metagenomics has been applied extensively to exploit concealed potential of microbial communities from almost all sorts of habitats. A brief historic progress made over the period is discussed in terms of origin of metagenomics to its current state and also the discovery of novel biological functions of commercial importance from metagenomes of diverse habitats. The present review also highlights the paradigm shift of metagenomics from basic study of community composition to insight into the microbial community dynamics for harnessing the full potential of uncultured microbes with more emphasis on the implication of breakthrough developments, namely, Next Generation Sequencing, advanced bioinformatics tools, and systems biology.
Experiments were conducted both under in vitro and in situ conditions to determine the biodegradation potential of button mushroom spent substrate (SMS) and its dominating microbes (fungi and bacteria) for carbendazim and mancozeb, the commonly used agricultural fungicides. During 6 days of incubation at 30 ± 2°C under broth culture conditions, highest degradation of carbendazim (17.45%) was recorded with B-1 bacterial isolate, while highest degradation of mancozeb (18.05%) was recorded with Trichoderma sp. In fungicide premixed sterilized SMS, highest degradation of carbendazim (100.00-66.50 lg g -1 ) was recorded with mixed
Simple sequence repeats are predominantly found in most organisms. They play
a major role in studies of genetic diversity, and are useful as diagnostic markers
for many diseases. The simple sequence repeats database (SSRD) for the human
genome was created for easy access to such repeats, for analysis, and to be used
to understand their biological significance. The data includes the abundance and
distribution of SSRs in the coding and non-coding regions of the genome, as well as
their association with the UTRs of genes. The exact locations of repeats with respect
to genomic regions (such as UTRs, exons, introns or intergenic regions) and their
association with STS markers are also highlighted. The resource will facilitate repeat
sequence analysis in the human genome and the understanding of the functional and
evolutionary significance of simple sequence repeats. SSRD is available through two
websites, http://www.ccmb.res.in/ssr and http://www.ingenovis.com/ssr.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.