DISCUSSION PAPER: OUABAIN‐INDUCED INHIBITION OF CARDIAC (N<scp>a</scp><sup>+</sup>+ K<sup>+</sup>)‐ATP<scp>ase</scp> AND THE POSITIVE INOTROPIC RESPONSE

Brody, Theodore M.

doi:10.1111/j.1749-6632.1974.tb19127.x

Cited by 8 publications

(3 citation statements)

References 4 publications

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“…The extracellular concentration of these ions in large part determines the intracellular concentration (McGrady & Nelson, 1973). The cardiac glycoside ouabain, an inhibitor of cardiac Na+-K+ ATPase (Brody, 1974), has been shown to bind to Na+-K+-activated ATPase sites in the midpiece-tail of bull spermatozoa (O'Donnell & Ellory, 1970). As part of our efforts to investigate the permeability properties of mature and developing spermatozoa, the present study of freshly ejaculated bull spermatozoa considered the effect of ouabain on membrane potential, frequency of the flagellar beat and maximum amplitude of the flagellar wave in order to (a) determine the contribution of ouabainsensitive Na+-K+ transport to membrane potential in bull spermatozoa, and (b) clarify the effects of ouabain on specific characteristics of motility.…”

Section: Introductionmentioning

confidence: 99%

The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa

McGrady¹

1979

Reproduction

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Ejaculated bull spermatozoa were exposed to various concentrations of ouabain to ascertain the effect on membrane potential, intracellular concentrations of sodium and potassium and motility. Membrane potenital, measured by electrophysiological methods, decreased. Intracellular potassium decreased and intracellular sodium increased. Progressive motility decreased. In addition, the motility change produced by ouabain was identified as a decrease both in beat frequency and in wave amplitude.

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Section: Introductionmentioning

confidence: 99%

The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa

McGrady¹

1979

Reproduction

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“…This inhibition accounts for its toxic and lethal effects, considering the widespread presence of this pump in all animal cells [ 6 ]. However, when administered at controlled doses, ouabain induces inhibition of Na + /K + -ATPase, leading to what is known as a positive inotropic effect, which explains its properties in cardiac tissue [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Ouabain Induces Transcript Changes and Activation of RhoA/ROCK Signaling in Cultured Epithelial Cells (MDCK)

Martínez-Rendón,

Hinojosa,

Xoconostle-Cázares

et al. 2023

CIMB

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Ouabain, an organic compound with the ability to strengthen the contraction of the heart muscle, was originally derived from plants. It has been observed that certain mammalian species, including humans, naturally produce ouabain, leading to its classification as a new type of hormone. When ouabain binds to Na+/K+-ATPase, it elicits various physiological effects, although these effects are not well characterized. Previous studies have demonstrated that ouabain, within the concentration range found naturally in the body (10 nmol/L), affects the polarity of epithelial cells and their intercellular contacts, such as tight junctions, adherens junctions, and gap junctional communication. This is achieved by activating signaling pathways involving cSrc and Erk1/2. To further investigate the effects of ouabain within the hormonally relevant concentration range (10 nmol/L), mRNA-seq, a high-throughput sequencing technique, was employed to identify differentially expressed transcripts. The discovery that the transcript encoding MYO9A was among the genes affected prompted an exploration of whether RhoA and its downstream effector ROCK were involved in the signaling pathways through which ouabain influences cell-to-cell contacts in epithelial cells. Supporting this hypothesis, this study reveals the following: (1) Ouabain increases the activation of RhoA. (2) Treatment with inhibitors of RhoA activation (Y27) and ROCK (C3) eliminates the enhancing effect of ouabain on the tight junction seal and intercellular communication via gap junctions. These findings further support the notion that ouabain acts as a hormone to emphasize the epithelial phenotype.

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“…The in vitro effect of exogenous digoxin on normal human spermatozoa progressive motility Ouabain, a digitalis related compound which is known to inhibit the Na-K ATPase pump in vitro (Brody, 1974), caused severe impairment of sperm motility and acrosome reaction (Nelson & McGrady, 1981). Chulavatnatol & Yiudepit (1976) demonstrated an increase in Na-K ATPase sensitivity to ouabain inhibition in rat sperm, when it migrates from the epididymal head to its cauda.…”

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confidence: 99%

The in vitro effect of exogenous digoxin on normal human spermatozoa progressive motility [letter]

Krivoy¹,

Vadasz²,

Stoler³

et al. 1992

Brit J Clinical Pharma

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DISCUSSION PAPER: OUABAIN‐INDUCED INHIBITION OF CARDIAC (Na⁺+ K⁺)‐ATPase AND THE POSITIVE INOTROPIC RESPONSE

Cited by 8 publications

References 4 publications

The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa

The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa

Ouabain Induces Transcript Changes and Activation of RhoA/ROCK Signaling in Cultured Epithelial Cells (MDCK)

The in vitro effect of exogenous digoxin on normal human spermatozoa progressive motility [letter]

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DISCUSSION PAPER: OUABAIN‐INDUCED INHIBITION OF CARDIAC (Na++ K+)‐ATPase AND THE POSITIVE INOTROPIC RESPONSE

Cited by 8 publications

References 4 publications

The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa

The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa

Ouabain Induces Transcript Changes and Activation of RhoA/ROCK Signaling in Cultured Epithelial Cells (MDCK)

The in vitro effect of exogenous digoxin on normal human spermatozoa progressive motility [letter]

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DISCUSSION PAPER: OUABAIN‐INDUCED INHIBITION OF CARDIAC (Na⁺+ K⁺)‐ATPase AND THE POSITIVE INOTROPIC RESPONSE