The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa

McGrady, Angele

doi:10.1530/jrf.0.0560549

Cited by 29 publications

(18 citation statements)

References 14 publications

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“…These differences in results between bull and rat spermatozoa [5] may be caused by the differences in species (rat vs. bull) and sources of spermatozoa (epididymal vs. ejaculated). It has been reported that 1 mM ouabain inhibited progressive motility of bovine sperm within 30 min [43]. In our study, a HamiltonThorn motility analyzer was used for motility evaluation, which detected a statistically significant reduction in progressive motility after 5 h of incubation under capacitating conditions, thus confirming the previously reported [43] impact of Na þ /K þ ATPase impact on sperm motility parameters.…”

supporting

confidence: 90%

“…This is NA þ /K þ ATPASE AND BOVINE SPERM CAPACITATION particularly relevant considering that inhibition of Na þ / K þ ATPase induces depolarization of sperm membrane, an event associated with acrosome reaction [43], and that acrosomal exocytosis of spermatozoa following incubation in the presence of ouabain is independent of extracellular calcium levels. Therefore, inhibition of Na þ /K þ ATPase and associated tyrosine phosphorylation may ''prematurely'' induce acrosome reaction before sperm complete the capacitation process.…”

Section: Inhibition Of Na þ /K þ Atpase Depolarizes Sperm Plasma Membmentioning

confidence: 99%

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Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation1

Thundathil

Anzar

Buhr

2006

Biology of Reproduction

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A heteromeric integral membrane protein, Na+/K+ATPase is composed of two polypeptides, alpha and beta, and is active in many cell types, including testis and spermatozoa. It is a well-known ion transporter, but binding of ouabain, a specific inhibitor of Na+/K+ATPase, to Na+/K+ATPase in somatic cells initiates responses that are similar to signaling events associated with bovine sperm capacitation. The objectives of the present study were to demonstrate the presence of Na+/K+ATPase in bovine sperm and to investigate its role in the regulation of bovine sperm capacitation. The presence of Na+/K+ATPase in sperm from mature Holstein bulls was demonstrated by immunoblotting and immunocytochemistry using a monoclonal antibody developed in mouse against the beta 1 polypeptide of Na+/K+ATPase. Binding of ouabain to Na+/K+ATPase inhibited motility (decreased progressive motility, average path velocity, and curvilinear velocity) and induced tyrosine phosphorylation and capacitation but did not increase intracellular calcium levels in spermatozoa. Furthermore, binding of ouabain to Na+/K+ATPase induced depolarization of sperm plasma membrane. Therefore, binding of ouabain to Na+/K+ATPase induced sperm capacitation through depolarization of sperm plasma membrane and signaling via the tyrosine phosphorylation pathway without an appreciable increase in intracellular calcium. To our knowledge, this is the first report concerning the signaling role of Na+/K+ATPase in mammalian sperm capacitation.

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supporting

confidence: 90%

Section: Inhibition Of Na þ /K þ Atpase Depolarizes Sperm Plasma Membmentioning

confidence: 99%

Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation1

Thundathil

Anzar

Buhr

2006

Biology of Reproduction

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“…In this species, ouabain does not modify total motility or amplitude of lateral head displacement, but it decreases progressive motility, and curvilinear and average path velocities (Thundathil et al 2006). On the other hand, McGrady (1979) found ouabain to cause a general decrease in bull sperm flagellar wave. Our results obtained from rats show that the inhibitory effect of ouabain extends to a wide range of sperm motility parameters.…”

Section: Nak-atpase A4 Isoform Mechanisms Of Actionmentioning

confidence: 61%

Activity of the Na,K-ATPase α4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa

Jiménez

Sánchez²,

Wertheimer³

et al. 2010

Reproduction

104

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While the function of the ubiquitous Na,K-ATPase a1 subunit has been well documented, the role of the sperm-specific a4 isoform of this ion transporter is less known. We have explored the importance of a4 in rat sperm physiology by taking advantage of the high sensitivity of this isoform for the inhibitor ouabain. Using concentrations that selectively block a4 activity, we found ouabain to reduce not only sperm total motility, but also multiple parameters of sperm movement, including progressive motility, straight line, curvilinear, and average path velocities, lateral head displacement, beat cross frequency, and linearity. According to a direct role of a4 in Na C transport, ouabain inhibition of a4 increased [Na C ] i in the male gametes. In addition, interference of a4 activity with ouabain produced cell membrane depolarization, diminished pH, and increased [Ca 2C ] i in spermatozoa. Inhibition of a4 was sufficient to cause all these effects and additional blockage of a1, the other Na,K-ATPase a isoform expressed in sperm, and higher doses of ouabain did not result in further changes in the cell parameters studied. These results show that a4 is the Na,K-ATPase isoform primarily involved in controlling the transmembrane Na C gradient in sperm, and that a4 activity is necessary for maintaining membrane potential, [Ca ] i , and acid-base balance suggests that their regulation is the mechanism by which a4 maintains motility of the male gametes.

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“…ouabain) is associated with an im paired spermatozoa motility [1], This finding which was demonstrated in more than one animal species [2,3] is controversial in humans [1]. In our laboratories we failed to demonstrate any effect of digoxin in vitro on normal human spermatozoa functions [unpubl.…”

Section: Introductionmentioning

confidence: 91%

“…The impaired spermatozoa motility in animals is thought to be related to the inhibition of the Na-K ATPase located mainly in the midpiece and tail regions of the spermatozoa membrane [2], Endogenous digoxin-like immunoreactivity (EDLI) was detected in different physiological and pathological conditions [4][5][6]11] and in several biological fluids [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Endogenous Digoxin-Like Immunoreactivity Measured in Seminal Fluid from a Normal Male Population

Vadazs

Jakobi²,

Stoler

et al. 1992

Gynecol Obstet Invest

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Endogenous digoxin-like immunoreactivity (EDLI) has been detected in different biological fluids and in several pathophysiological conditions. In this study, using radioimmunoassay we reported for the first time the existence of bound and unbound EDLI in normal seminal fluid. The unusual finding was the detection of unbound EDLI in the seminal fluid, while this reactivity was undetected in plasma. Two main hypotheses are presented: (1) local secretion of unbound EDLI and/or (2) passive diffusion from plasma to the seminal fluid of unbound EDLI and subsequent local concentration.

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The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa

Cited by 29 publications

References 14 publications

Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation1

Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation1

Activity of the Na,K-ATPase α4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa

Endogenous Digoxin-Like Immunoreactivity Measured in Seminal Fluid from a Normal Male Population

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