Discriminatory proteomic biomarker analysis identifies free hemoglobin in the cerebrospinal fluid of women with severe preeclampsia

Norwitz, Errol R.; Tsen, Lawrence C.; Park, Joong Shin; Fitzpatrick, Patricia A.; Dorfman, David M.; Saade, George R.; Buhimschi, Catalin S.; Buhimschi, Irina A.

doi:10.1016/j.ajog.2005.06.055

Cited by 42 publications

(39 citation statements)

References 14 publications

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“…Proteomics is a recently developed tool that has the characteristics of having high-throughput and being highly sensitive. To investigate the pathogenesis and protein markers of preeclampsia, proteomics has been employed by some researchers to explore the peripheral blood [5], cerebrospinal fluid [6], urine [7], amniotic fluid [8] and placental trophoblasts [9]. To date, no study has been conducted to investigate the decidual tissues of women with preeclampsia with proteomics.…”

Section: Discussionmentioning

confidence: 99%

Alpha-1-Antitrypsin Acts as a Preeclampsia-Related Protein: A Proteomic Study

Feng

Zhou²,

Li³

et al. 2012

Gynecol Obstet Invest

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Aims: To screen the preeclampsia-related protein by proteomics. Methods: Proteomics was performed to identify differential protein expression profiles between normal full-term pregnancy, early-onset severe preeclampsia (ES-PE) or late-onset severe preeclampsia (LS-PE; n = 10 per group). Real-time quantitative PCR and immunohistochemistry were conducted to confirm the expression of α₁-antitrypsin (α₁-AT) in the decidual tissues of different subjects. ELISA was employed to detect the α₁-AT content in the peripheral blood of 90 women (n = 30 per group). Results: We successfully constructed two-dimensional electrophoresis maps of decidual tissues, and a total of 20 differentially expressed proteins were identified. The α₁-AT expression was different among the three groups. The normal full-term pregnancy women expressed the most α₁-AT, and the LS-PE women expressed the least amount of α₁-AT. The difference in the α₁-AT expression was consistent with the proteomics data. The peripheral α₁-AT content was the highest in the normal full-term pregnancy group (1.85 ± 0.15 g/l), moderate in the ES-PE group (0.77 ± 0.14 g/l) and lowest in the LS-PE group (0.42 ± 0.07 g/l; p < 0.05). Conclusion: Using 2D PAGE, we identified twenty proteins with significantly altered expression in PE. These differentially expressed proteins include prevention protein, in which α₁-AT is downregulated in PE.

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Section: Discussionmentioning

confidence: 99%

Alpha-1-Antitrypsin Acts as a Preeclampsia-Related Protein: A Proteomic Study

Feng

Zhou²,

Li³

et al. 2012

Gynecol Obstet Invest

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“…Two tumor-related proteins, N-myc oncoprotein and low-molecular weight caldesmon, were identified in CSF samples of patients with primary brain tumors [356]. The CSFs of patients suffering from HIV, Chronic Fatigue Syndrome (CFS), preeclampsia, and cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy were also investigated [357][358][359][360]. Assessment of CSF fluids from CFS, Persian Gulf War Illness (PGI), and fibromyalgia patients by LC-QTOF-MS suggested that the CSF proteomes and presumably pathological mechanisms might be shared among these disorders although syndrome names and definitions are different.…”

Section: Application To Neurological Disease Detectionmentioning

confidence: 99%

Human body fluid proteome analysis

2006

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The focus of this article is to review the recent advances in proteome analysis of human body fluids, including plasma/serum, urine, cerebrospinal fluid, saliva, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate fluid, tear fluid, and amniotic fluid, as well as its applications to human disease biomarker discovery. We aim to summarize the proteomics technologies currently used for global identification and quantification of body fluid proteins, and elaborate the putative biomarkers discovered for a variety of human diseases through human body fluid proteome (HBFP) analysis. Some critical concerns and perspectives in this emerging field are also discussed. With the advances made in proteomics technologies, the impact of HBFP analysis in the search for clinically relevant disease biomarkers would be realized in the future.

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“…Currently, only limited advantage has been taken from this new approach in preeclampsia-related material. One observation was made in cerebrospinal fluid of women with severe preeclampsia, where small amounts of free hemoglobin were detected in contrast to mild preeclampsia or normotensive controls (Norwitz et al, 2005). As proof of principle, a differential expression pattern of 5 proteins in plasma samples of preeclamptic compared to normal pregnant women at 26th weeks of gestation was found, yet the nature of the proteins was not communicated (Myers et al, 2004).…”

Section: Genetics and Hypothesis-generating Approachesmentioning

confidence: 95%

Molecular aspects of preeclampsia

Mohaupt¹

2007

Molecular Aspects of Medicine

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Discriminatory proteomic biomarker analysis identifies free hemoglobin in the cerebrospinal fluid of women with severe preeclampsia

Cited by 42 publications

References 14 publications

Alpha-1-Antitrypsin Acts as a Preeclampsia-Related Protein: A Proteomic Study

Alpha-1-Antitrypsin Acts as a Preeclampsia-Related Protein: A Proteomic Study

Human body fluid proteome analysis

Molecular aspects of preeclampsia

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