Discrimination of zygotic and nucellar seedlings of five polyembryonic citrus rootstocks by isozyme analysis and seedling morphology

Ashari, Sumeru; Aspinall, D.; Sedgley, Margaret

doi:10.1080/14620316.1988.11515912

Cited by 24 publications

(12 citation statements)

References 2 publications

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“…Isoenzyme analysis has found similar genetic variability at the biochemical level even 234 banding, CMA/DAPI staining, heterochromatin, in morphologically uniform seedling populations (Moore & Castle, 1988). Such an approach has also been useful to distinguish zygotic from nucellar seedlings (Iglesias et al, 1974;Ashari et at., 1988). As we are concerned with the cytogenetic analysis of the genus, we are interested in identifying its chromosomal variability and in looking for a further approach to the genetic characterization of species or clones of Citrus.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining

Guerra

1993

Heredity

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The CMA/DAPI chromosome banding pattern of six Citrus species was analysed. All of them showed a large amount of heterochromatin and heterozygosity. Most of the heterochromatin was CMA+ /DAPI -and was located mainly in the terminal region of the long arms. Each individual was heterozygous for at least one chromosome pair and each species presented a different banding pattern. The greatest amount of information on karyotype variation can be obtained from the four largest pairs. Pairs I and III showed the highest amount of heterochromatin and heterozygosity whereas pair II was highly stable and almost completely lacked CMA+ heterochromatin. On the other hand, some features common to all species suggest a strong chromosome homoeology conservation. The CMA staining revealed some chromosome markers for each Citrus species that may be very useful in the identification of zygotic versus nucellar embryos, as well as for further evolutionary studies.

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Section: Introductionmentioning

confidence: 99%

Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining

Guerra

1993

Heredity

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“…Andrade-Rodriguez et al (2004) were able to classify 26% of seedlings from polyembryonic seeds of C. volkameriana as zygotics, whereas Bastianel et al (1998) (1988) used isozyme markers to identify a wide range of frequency of zygotics, 5.6 to 50.6%, among the 12 different citrus rootstock accessions screened. Studies by Ashari et al (1988) reported as low as 0% of zygotic seedlings in Cleopatra mandarin using isozymes and morphological characteristics. The percentages of zygotic progenies in various citrus hybrids have been found to depend on the seed parent used (Spiegel-Roy et al 1977), the pollen origin (Cameron and Soost 1969), and environmental influences (Moore and Castle 1988).…”

Section: Discussionmentioning

confidence: 97%

“…Various techniques have been used to distinguish nucellar from zygotic offspring including colorimetric assays (Furr and Reece 1946), infrared spectroscopy (Pieringer and Edwards 1967), enzymatic darkening of polyphenols (Esen and Soost 1974), chromatography (Weinbaum et al 1982), and isozyme pattern analysis (Ashari et al 1988; Moore and Castle 1988;Xiang and Roose 1988;Tusa et al 2002). However, none of these methods efficiently provide sufficient confirmation of the identity of true nucellar seedlings (Ruiz et al 2000;Tusa et al 2002).…”

Section: Introductionmentioning

confidence: 98%

Characterization of zygotic and nucellar seedlings from sour orange-like citrus rootstock candidates using RAPD and EST-SSR markers

Rao

Soneji

Chen

et al. 2007

Tree Genetics & Genomes

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A series of crosses designed for introgression of mandarin (Citrus reticulata Blanco) and pummelo (C. maxima Merr.) germplasm, to develop an alternative rootstock to sour orange (C. aurantium L.), were carried out. It is necessary to identify those hybrids that yield nucellar seedlings for rootstock propagation. Rootstocks can be developed through traditional plant breeding methods; however, the ability to screen and select for economically important traits (such as production of true nucellar seedlings) in an efficient fashion is limited by the difficulties of screening techniques based on whole plant performance. To address these problems, we have used randomly amplified polymorphic DNA (RAPD) and fluorescently labeled expressed sequence tag simple sequence repeat (EST-SSR) molecular markers. A total of 204 individual seedlings obtained from 34 hybrid parental plants were successfully characterized using five RAPD primers. Ten hybrid parents and their progenies, found to be genetically similar among themselves, were selected for more scrutiny using eight EST-SSR primer pairs. The degrees of genetic similarity (nucellars) among progeny seedlings were determined and compared with that of their parents. The mean genetic similarity varied from 67-99% among the selected rootstock candidates screened. The genetic similarity relationship identified using RAPD and EST-SSR molecular markers was highly concordant (p=0.001). Two elite rootstock candidates (B6R5T56; B6R11T129) that seem to be ideal for future mandarin and pummelo derived rootstock breeding programs have been identified. Our results indicate that either RAPD or EST-SSR analyses could be equally successful in identifying true nucellars among the progenies obtained from introgression crosses of mandarin and pummelo, thus improving the accuracy of early selection in a citrus rootstock breeding program.

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“…, 적외선 분광법 (Pieringer and Edwards, 1967), 박층크로마토그래피 (Tatum et al, 1978), 크로마토그래피 (Weinbaum et al, 1982), 그리고 동질효소 패턴분석법 (Ashari et al, 1988;Moore and Castle, 1988;Moon et al, 1991;Tusa et al, 2002) 등이 사용되었으나 이 들 방법은 식물체 상태, 환경적 조건에 따라서 영향을 받기 때문에 실험자에 따라 동일한 결과를 얻지 못하는 문제점이 있다. 따라서, 이 방법들은 감귤 교배육종에서 교잡종 실생 묘를 선발하기 위한 일반적인 방법으로는 효과적이지 못하 다 (Ruiz et al, 2000;Tusa et al, 2002).…”

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Early Identification of Citrus Zygotic Seedlings Using Pollen-specific Molecular Markers

Jin¹,

Yun²,

Park³

et al. 2015

HST

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This study was carried out to develop molecular techniques to allow the selection of zygotic seedlings in the early stage of the plant development. We identified 37 pollen-specific molecular markers from RAPD analysis and successfully used them for identification of the zygotic seedlings from various hybrid crosses. Three Satsuma mandarin cultivars ('Morita unshiu', 'Nangan 20' and 'Miyagawawase') were used as mother parents and seven cultivars ('Ponkan', 'Lee', 'Kinokuni', 'Shiranuhi', 'Tamnaneunbong', 'Shinyegam', and 'Sunburst' mandarins) served as pollen parents. PCR analysis showed that 2 primers could identify zygotic hybrid seedlings. Among them, an UBC-27 primer was used to identify the zygotic seedlings from hybrid crosses of "'Nangan 20' × 'Kinokuni'" mandarin, "'Nangan 20 × Ponkan'" mandarin and "'Miyagawawase × Sunburst'" tangerine. In total 29 out of 40 seedlings (73%), 9 out of 47 seedlings (19%), and 13 out of 45 (29%) were identified as zygotic seedlings, respectively. These results can show that the pollen-specific markers selected in this study can be used effectively for early identification of zygotic seedlings from Citrus hybrid crosses.

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Discrimination of zygotic and nucellar seedlings of five polyembryonic citrus rootstocks by isozyme analysis and seedling morphology

Cited by 24 publications

References 2 publications

Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining

Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining

Characterization of zygotic and nucellar seedlings from sour orange-like citrus rootstock candidates using RAPD and EST-SSR markers

Early Identification of Citrus Zygotic Seedlings Using Pollen-specific Molecular Markers

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