Discrimination of three Scrophularia plants utilizing ‘Scrophularia Radix’ by DNA markers based on internal transcribed spacer (ITS) sequences

Abstract

One of the medicinal materials produced by plants belonging to the genus Scrophularia,

Systematic position of Oreosolen (tribe Scrophularieae, Scrophulariaceae) based on nuclear and plastid sequences

“…Each fragment was sequenced for both strands. Thirty-two newly generated DNA sequences have been deposited in GenBank, whereas the other sequences were produced in the previous studies (Kornhall et al, 2001;Wolfe et al, 2002;Kornhall & Bremer, 2004;Albach et al, 2005;Oxelman et al, 2005;Han et al, 2009;Lee et al, 2010;Attar et al, 2011;Scheunert & Heubl, 2011, 2014Navarro-P erez et al, 2013Navarro-P erez et al, , 2015 and retrieved from GenBank (Table S1).…”

Multiple windows of colonization to Macaronesia by the dispersal-unspecialized Scrophularia since the Late Miocene

“…A total of 102 sequences of Scrophularia and two of Verbascum from both ITS and trnL-trnF regions were taken from our previous study (Navarro-Pérez et al, 2013). Fifty-eight additional ITS, trnL-trnF, psbA-trnH and trnQ-rps16 sequences of Scrophularia were taken from the GenBank database (Attar et al, 2011;Han et al, 2009;Lee et al, 2010;Heubl, 2011, 2014), as well as twelve sequences of related genera (Teedia, Manulea, Table 1 Groups of native Macaronesian species and subspecies of Scrophularia defined by Dalgaard (1979) (Kornhall and Bremer, 2004;Kornhall et al, 2001;Oxelman et al, 2005;Heubl, 2011, 2014;Wolfe et al, 2002) (see Table S1 for accession numbers). All newly generated sequences were submitted to the GenBank database (see Table S1 for accession numbers).…”

Correlation analyses between molecular perspective and phytochemical variations in Coptis chinensis Franch

“…The ITS region of nrDNA has been employed to assist identification of the traditional Chinese medicinal plants (Xie et al, 2009;Lee et al, 2010). In recent years, the interspecific relationships of genus Coptis have been reported (He et al, 2014), however, to date, no report showed the intraspecific relationship of C. chinensis.…”