Discrimination of motile bacteria from filamentous fungi using dynamic speckle

Murialdo, Silvia Elena; Passoni, Lucía Isabel; Guzmán, Marcelo Nicolás; Sendra, Gonzalo Hernán; Rabal, H.; Trivi, Marcelo; Gonzalez, Jorge Froilán

doi:10.1117/1.jbo.17.5.056011

Cited by 23 publications

(17 citation statements)

References 14 publications

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“…12). Results are in qualitative agreement with those found in a previous work (for details and comparison see [11]). The region occupied by bacteria can be distinguished, as well as the fungus but with a considerable lower activity (see inside the red circle).…”

Section: B Activity Imagessupporting

confidence: 93%

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Island analysis of low-activity dynamic speckles

et al. 2013

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In this work we present a method to evaluate activity in low dynamic speckle patterns. It consists of binarizing the speckle image and analyzing the displacements and deformations of the resulting speckle grain regions, here called islands. Numerical simulations and controlled experiments were used to study the variations of the island features with the aim of finding a correlation with the activity of the speckle pattern. From the obtained results it was possible to conclude that the developed method can be useful for the analysis of low activity speckle patterns with the advantage of requiring only pairs of frames, thus permitting the assessment of nonstationary processes. In the case of stationary phenomena, so that stacks of frames registers are representative of them, dilute activity images can also be constructed.

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Section: B Activity Imagessupporting

confidence: 93%

“…The same procedure was also tested with a set of dynamic speckle images obtained from a biological sample (as in [11]). …”

Section: B Activity Imagesmentioning

confidence: 99%

Island analysis of low-activity dynamic speckles

et al. 2013

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“…The chemotactic images obtained recently with speckle laser (Murialdo et al, 2009(Murialdo et al, , 2012, were applied in this work for the first time to investigate chemotaxis morphology in presence of non-polar pollutants as attractants. We showed the effectiveness of using two complementary techniques with white light and dynamic speckle laser plus the FFM analysis for chemotactic morphology studies.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

Dynamic laser speckle and fuzzy mathematical morphology applied to studies of chemotaxis towards hydrocarbons

Nisenbaum

Bouchet

Guzmán

et al. 2014

IJENVH

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Abstract:The movement of the microorganisms towards a higher concentration of the chemical attractant is called positive chemotaxis and is involved in the efficiency of chemical degradation. Several studies are focused in this field related to genomics, and towards demonstrating chemotactic responses by bacteria, but there is little information related to the activity and morphology of their response. In this work, we use a recently reported dynamic speckle laser method, to process images and to distinguish motile surface patterns per area of colonisation by applying image processing techniques called fuzzy mathematical morphology (FMM). The images of bacterial colonies are usually surfaced, with vague edges and non-homogeneous grey levels. Hence, conventional image processing methods for shape analysis cannot be applied in these cases. In this paper, we propose the application FMM to solve this problem. The approach given was effective to segment, detect and also to describe colonisation patterns. 60M. Nisenbaum et al.

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“…It is an interference optical phenomenon, which presents information about the analyzed surface, and it may be used to identify fungi and bacteria [11].…”

Section: David Publishingmentioning

confidence: 99%

Maize Seeds Submitted to Thermotherapy and Analyzed by Dynamic Speckle

Peixoto¹,

Silva²,

Fujii³

et al. 2018

JAST-B

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The quality of the seeds used for sowing is fundamental for a high productivity and quality of the crop. Therefore, eradicating pathogens is essential for the plant to develop in the best possible way. The eradication of those pathogens can be accomplished by means of treatments that consist of the application of substances to the seeds or by performing physical procedures. Thermotherapy is a physical treatment, in which the seed is exposed to a given thermal energy, such as heated air or steam, and can be highly efficient for controlling those pathogens, in addition to reducing environmental damage and costs. The present work aimed to evaluate the efficiency of thermotherapy in two ways: wet heat (water) and dry heat (water vapor), both at 60 °C, alternating the exposure time to the thermal sources. The treatment with humid heat with a time of exposure of 5 min was the most efficient, since it presented higher germination percentages (83%) and less occurrence of Fusarium sp. (33%). It was observed that the most efficient thermotherapy is in the control of Fusarium, the most aggressive is for the quality of the seed. The quality of the treatments was evaluated through standard methodologies, according to the rules for seed analysis, such as germination and sanitation tests. Biospeckle laser was used to identify the microbial activity in these seeds, through activity maps generated by laser speckle contrast analysis (LASCA) processing. In this way, the research involving thermotherapy can be done by varying the temperature and the time of exposure to obtain values that considerably reduce the pathogenic agents and preserve the quality, in order to maintain the values of germination and vigor acceptable for commercialization.

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Discrimination of motile bacteria from filamentous fungi using dynamic speckle

Cited by 23 publications

References 14 publications

Island analysis of low-activity dynamic speckles

Island analysis of low-activity dynamic speckles

Dynamic laser speckle and fuzzy mathematical morphology applied to studies of chemotaxis towards hydrocarbons

Maize Seeds Submitted to Thermotherapy and Analyzed by Dynamic Speckle

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