Discrimination, classification, identification of microorganisms using FTIR spectroscopy and chemometrics

Mariey, Laurence; Signolle, J. P.; Amiel, Caroline; Travert, J.

doi:10.1016/s0924-2031(01)00113-8

Cited by 310 publications

(209 citation statements)

References 56 publications

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“…Advances in the 1990s were favored by the development of interferometric IR spectroscopy and Fourier transform (FT) techniques. The reference work of Helm and colleagues (14) prompted a large amount of research, reviewed by Mariey and colleagues (26). Since 2001, more groups and genera have been studied: Lactobacillus (3,36,37), Carnobacterium (21), Bacillus (1), Brucella (27), Acinetobacter (52), yeasts (20,41), enterococci (7,10,19), coryneforms (24,34), and actinomycetes (54).…”

mentioning

confidence: 99%

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Mouwen

Weijtens

Capita

et al. 2005

Appl Environ Microbiol

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Fourier transform infrared spectroscopy (FT-IR) has been used together with pattern recognition methodology to study isolates belonging to the species Campylobacter coli and Campylobacter jejuni and to compare FT-IR typing schemes with established genomic profiles based on enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Seventeen isolates were cultivated under standardized conditions for 2, 3, and 4 days to study variability and improve reproducibility. ERIC-PCR profiles and FT-IR spectra were obtained from strains belonging to the species Campylobacter coli and C. jejuni, normalized, and explored by hierarchical clustering and stepwise discriminant analysis. Strains could be differentiated by using mainly the firstderivative FT-IR spectral range, 1,200 to 900 cm ؊1 (described as the carbohydrate region). The reproducibility index varied depending on the ages of the cultures and on the spectral ranges investigated. Classification obtained by FT-IR spectroscopy provided valuable taxonomic information and was mostly in agreement with data from the genotypic method, ERIC-PCR. The classification functions obtained from the discriminant analysis allowed the identification of 98.72% of isolates from the validation set. FT-IR can serve as a valuable tool in the classification, identification, and typing of thermophilic Campylobacter isolates, and a number of types can be differentiated by means of FT-IR spectroscopy.

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confidence: 99%

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Mouwen

Weijtens

Capita

et al. 2005

Appl Environ Microbiol

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“…The FTIR spectral chemometrics of different Termitomyces species showed sub grouping with other species may be due to heterogeneity of chemical components in different pellet zones. Grouping various strains to form clusters representing species or genera based on taxonomical classification is not always satisfactory by using physiological and morphological characters [48,49]. The chemical heterogeneity between these species may be affected by the geographic origin, morphology, physiology of culture and ability for metabolite production.…”

Section: Resultsmentioning

confidence: 99%

Potential of FTIR spectroscopy in chemical characterization of Termitomyces Pellets

2017

J App Biol Biotech

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Potential of Fourier-Transform Infrared (FTIR) spectroscopy was assessed and Hierarchical Cluster Analysis (HCA) was applied over entire range of spectra for determining characteristic chemical compositional similarity of 11 different Termitomyces Heim strains using 20 days old pelletized dry biomass produced under submerged shaken condition at 28±1 o C. Four dominant spectral windows showing C-H, O-H stretching region, amide I, amide II and polysaccharide regions were identified displaying characteristic variable bands across species at 3400-3200 cm -1 , 2900-2850 cm -1 , 2350-2215 cm -1 , 1750 cm -1 , 1658-1625 cm -1 , 1582-1547 cm -1 , 1375-1315 cm -1 and 900-725 cm -1 . The HCA dendrogram showed formation of two major clusters based on their presumptive chemical similarity.

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“…Even different strains within a species can be distinguished (Naumann et al 1991, Mariey et al 2001, Ngo-Thi et al 2003. Fungal studies addressed first species with relevance to medicine and food industry, such as Candida albicans, Saccharomyces cerevisiae, and various edible and toxic mushrooms (Naumann et al 1991, Ngo-Thi et al 2003).…”

Section: Detection Of Fungi In Woodmentioning

confidence: 99%

Wood production, wood technology, and biotechnological impacts

Kües¹

2007

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Discrimination, classification, identification of microorganisms using FTIR spectroscopy and chemometrics

Cited by 310 publications

References 56 publications

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Potential of FTIR spectroscopy in chemical characterization of Termitomyces Pellets

Wood production, wood technology, and biotechnological impacts

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