Discrimination between protein glycoforms using lectin-functionalised gold nanoparticles as signal enhancers

Neves, Marta M.P.S.; Richards, Sarah‐Jane; Baker, Alexander N.; Walker, Marc; Georgiou, Panagiotis G.; Gibson, Matthew I.

doi:10.1039/d2nh00470d

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Lectins are natural proteins that are able to recognize bound monosaccharides, oligosaccharides, and glycans attached to the proteins, thus enabling recognition of the glycoproteins. Lectins have been utilized as recognition elements or bioreceptors in the fabrication of biosensors tailored for glycoprotein determination. ,, A general requirement of impedimetric biosensors is to utilize small bioreceptors for protein detection . Lectins are generally smaller than antibodies, and thus they are ideal candidates to serve as small bioreceptors, being able to form a denser biofilm on the sensor surface to enable sensitive detection.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Li,

2024

Langmuir

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An electrochemical impedimetric biosensing platform with lectin as a molecular recognition element has been established for the sensitive detection of glycoproteins, a class of important biomarkers in clinical diagnosis. One of the representative metal−organic framework materials, MIL-101(Cr)-NH 2 , was utilized as the supporting matrix, and its amino groups served as the anchors to immobilize the lectins of concanavalin A (Con A), constituting Con A@MIL-101(Cr)-NH 2 for the determination of invertase (INV) as a model glycoprotein. The Con A concentration, immobilization time, and incubation time with INV were optimized. Under the optimal conditions, the degree of impedance increase was linearly proportional to the logarithm of INV concentration between 1.0 × 10 −16 and 1.0 × 10 −11 M, affording a limit of detection as low as 3.98 × 10 −18 M. Good specificity, stability, reproducibility, and repeatability were demonstrated for the fabricated biosensing platform. Moreover, real mouse serum samples were spiked with different concentrations of INV. Excellent recoveries were obtained, which demonstrated the biosensing platform's capability of analyzing glycoproteins within a complex matrix.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Li,

2024

Langmuir

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Sugar-Coated: Can Multivalent Glycoconjugates Improve upon Nature’s Design?

Leslie,

Berry,

Miller

et al. 2024

J. Am. Chem. Soc.

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Multivalent interactions between receptors and glycans play an important role in many different biological processes, including pathogen infection, self-recognition, and the immune response. The growth in the number of tools and techniques toward the assembly of multivalent glycoconjugates means it is possible to create synthetic systems that more and more closely resemble the diversity and complexity we observe in nature. In this Perspective we present the background to the recognition and binding enabled by multivalent interactions in nature, and discuss the strategies used to construct synthetic glycoconjugate equivalents. We highlight key discoveries and the current state of the art in their applications to glycan arrays, vaccines, and other therapeutic and diagnostic tools, with an outlook toward some areas we believe are of most interest for future work in this area.

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Deciphering disease through glycan codes: leveraging lectin microarrays for clinical insights

Yang,

Lin,

et al. 2024

ABBS

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Glycosylation, a crucial posttranslational modification, plays a significant role in numerous physiological and pathological processes. Lectin microarrays, which leverage the high specificity of lectins for sugar binding, are ideally suited for profiling the glycan spectra of diverse and complex biological samples. In this review, we explore the evolution of lectin detection technologies, as well as the applications and challenges of lectin microarrays in analyzing the glycome profiles of various clinical samples, including serum, saliva, tissues, sperm, and urine. This review not only emphasizes significant advancements in the high-throughput analysis of polysaccharides but also provides insight into the potential of lectin microarrays for diagnosing and managing diseases such as tumors, autoimmune diseases, and chronic inflammation. We aim to provide a clear, concise, and comprehensive overview of the use of lectin microarrays in clinical settings, thereby assisting researchers in conducting clinical studies in glycobiology.

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Discrimination between protein glycoforms using lectin-functionalised gold nanoparticles as signal enhancers

Abstract: Biolayer interferometry is used to distinguish between glycoforms of the same using lectin-functional nanoparticles as both readers, and signal enhancers. The use of this is exemplified with prostate specific antigen.

Cited by 3 publications

References 38 publications

Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Sugar-Coated: Can Multivalent Glycoconjugates Improve upon Nature’s Design?

Deciphering disease through glycan codes: leveraging lectin microarrays for clinical insights

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