Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Abstract

An electrochemical impedimetric biosensing platform with lectin as a molecular recognition element has been established for the sensitive detection of glycoproteins, a class of important biomarkers in clinical diagnosis. One of the representative metal−organic framework materials, MIL-101(Cr)-NH 2 , was utilized as the supporting matrix, and its amino groups served as the anchors to immobilize the lectins of concanavalin A (Con A), constituting Con A@MIL-101(Cr)-NH 2 for the determination of invertase (INV) as a model glycoprotein. The Con A concentration, immobilization time, and incubation time with INV were optimized. Under the optimal conditions, the degree of impedance increase was linearly proportional to the logarithm of INV concentration between 1.0 × 10 −16 and 1.0 × 10 −11 M, affording a limit of detection as low as 3.98 × 10 −18 M. Good specificity, stability, reproducibility, and repeatability were demonstrated for the fabricated biosensing platform. Moreover, real mouse serum samples were spiked with different concentrations of INV. Excellent recoveries were obtained, which demonstrated the biosensing platform's capability of analyzing glycoproteins within a complex matrix.

Functional Interface for Glycoprotein Sensing: Focusing on Biosensors

“…However, lectins are able to exist in oligomer structures with multiple binding sites to simultaneously interact with different glycans of the same glycoprotein or with different arms of a branched oligosaccharide, thus achieving multivalent interaction to boost selectivity and affinity . Lectins have been utilized as capable recognition element to enable glycoprotein identification, profiling and sensing. − Tkac and co-workers have developed a series of lectin-based impedimetric biosensors for the sensitive detection and glycoprofiling of glycoproteins. , Recently, Qu and co-worker reported to modify lectin of concanavalin A (Con A) on metal–organic framework material of MIL-101­(Cr)-NH 2 to afford an electrochemical impedimetric biosensing platform of Con A@MIL-101­(Cr)-NH 2 for glycoprotein detection …”