Discovery of resistance to <i>Pratylenchus neglectus</i> among <i>P. thornei</i>‐resistant Iranian landrace wheats and the introgression of both resistances into advanced breeding lines

Sheedy, J. G.; Lin, J.; Thompson, John

doi:10.1111/ppa.13616

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2024

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Cited by 2 publications

References 30 publications

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Nematode problems in wheat and barley and their sustainable management

Owen¹,

Walia²,

Yan³

et al. 2023

Nematode Diseases of Crops and Their Sustainable Management

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Nematode problems in wheat and barley and their sustainable management

Owen¹,

Walia²,

Yan³

et al. 2023

Nematode Diseases of Crops and Their Sustainable Management

View full text Add to dashboard Cite

Differential induction of defense genes in hexaploid wheat roots by the plant-parasitic nematodes Pratylenchus neglectus and P. thornei

Okubara,

Sharpe,

Peetz

et al. 2024

PLoS ONE

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Pratylenchus neglectus and P. thornei are among the most destructive root lesion nematodes of wheat in the Pacific Northwest, United States of America and throughout the world. The aim of this study was to determine whether both nematode species were similar in their ability to induce defense genes in roots of wheat genotype Scarlet, and whether a combination of both species induced a different pattern of gene induction than each species alone. The long-term aspect of the research was to identify nematode-inducible promoters for deploying defense genes in roots in breeding programs. The root transcriptomes of genotype Scarlet were obtained after a one-week infection period with each nematode species separately, or both species combined. Root defense gene expression was induced for all three treatments relative to the no-nematode control, but P. thornei affected expression to a greater extent compared to P. neglectus. The species combination induced the highest number of defense genes. This result was not predicted from nematode enumeration studies, in which P. thornei colonization was substantially lower than that of P. neglectus, and the nematode combination did not show a significant difference. Quantitative real time polymerase chain reaction (qRT-PCR) assays for Dehydrin2, Glucan endo-1,3-beta-glucosidase, 1-cys-Peroxiredoxin, Pathogenesis-related protein 1 and Late embryogenesis-abundant proteins 76 and group 3 authenticated the induction observed in the transcriptome data. In addition, a near-isogenic line of Scarlet harboring genetic resistance to fungal soilborne pathogens, called Scarlet-Rz1, showed similar or higher levels of defense gene expression compared to fungus-susceptible Scarlet in qRT-PCR assays. Finally, transcriptome expression patterns revealed nematode-inducible promoters that are responsive to both P. neglectus and P. thornei.

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Discovery of resistance to Pratylenchus neglectus among P. thornei‐resistant Iranian landrace wheats and the introgression of both resistances into advanced breeding lines

Cited by 2 publications

References 30 publications

Nematode problems in wheat and barley and their sustainable management

Nematode problems in wheat and barley and their sustainable management

Differential induction of defense genes in hexaploid wheat roots by the plant-parasitic nematodes Pratylenchus neglectus and P. thornei

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