Discovery of Natural FabH Inhibitors Using an Immobilized Enzyme Column and Their Antibacterial Activity against <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>

Zhao, Shuangshuang; Xiao, Cheng; Wang, Jiajie; Tian, Kailin; Ji, Wenxia; Yang, Tingting; Khan, Bashir M.; Qian, Guoliang; Yan, Wei; Ye, Yonghao

doi:10.1021/acs.jafc.0c06363

Cited by 18 publications

(13 citation statements)

References 35 publications

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“…A new 4-hydroxycinnamic acid derivative compound, methyl 2-{(E)-2-[4-(formyloxy) phenyl]ethenyl}-4-methyl-3-oxopentanoate (146), along with the known compounds (3R,6R)-4-methyl-6-(1-methylethyl)-3-phenylmethylperhydro-1,4-oxazine-2,5-dione (147), (3R,6R)-N-methyl-N-(1-hydroxy-2-methylpropyl)-phenylalanine (148), siccanol (149), sambutoxin (150, Figure 9) and fusaproliferin (106), were extracted from Pyronema sp. an endophyte of the Taxus mairei.…”

Section: Minor Taxa Of the Ascomycetesmentioning

confidence: 99%

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Fungal Endophytes: A Potential Source of Antibacterial Compounds

Deshmukh

Dufossé²,

Chhipa

et al. 2022

JoF

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Antibiotic resistance is becoming a burning issue due to the frequent use of antibiotics for curing common bacterial infections, indicating that we are running out of effective antibiotics. This has been more obvious during recent corona pandemics. Similarly, enhancement of antimicrobial resistance (AMR) is strengthening the pathogenicity and virulence of infectious microbes. Endophytes have shown expression of various new many bioactive compounds with significant biological activities. Specifically, in endophytic fungi, bioactive metabolites with unique skeletons have been identified which could be helpful in the prevention of increasing antimicrobial resistance. The major classes of metabolites reported include anthraquinone, sesquiterpenoid, chromone, xanthone, phenols, quinones, quinolone, piperazine, coumarins and cyclic peptides. In the present review, we reported 451 bioactive metabolites isolated from various groups of endophytic fungi from January 2015 to April 2021 along with their antibacterial profiling, chemical structures and mode of action. In addition, we also discussed various methods including epigenetic modifications, co-culture, and OSMAC to induce silent gene clusters for the production of noble bioactive compounds in endophytic fungi.

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Section: Minor Taxa Of the Ascomycetesmentioning

confidence: 99%

“…Molecular modeling was then used to picture how these compounds interact with XooFabH. Compounds 44, and 343, displayed significant bactericidal activity against rice bacterial leaf blight with a protective efficiency of 66.2 and 82.5% at concentration of 200 µg/mL, respectively [148].…”

Section: Alternariamentioning

confidence: 99%

Fungal Endophytes: A Potential Source of Antibacterial Compounds

Deshmukh

Dufossé²,

Chhipa

et al. 2022

JoF

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“…Usage of enzymes to produce new compounds or modify antibacterial compounds is important (Zengin et al, 2018;Saleem et al, 2020;Zhao et al, 2020;Tukenova et al, 2021). Fungal laccases are an inexpensive and readily accessible enzyme for earning reaction components.…”

Section: Introductionmentioning

confidence: 99%

Testing for the ability to modify antibiotics of Panus tigrinus 8/18 Lentinus strigosus 1566 laccase

et al. 2024

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In advanced biotechnology, the utilization of enzymes to achieve new or modified compounds with antibacterial, fungicidal, and anti-cancer specifications is crucial. Mushroom lactases are a hopeful biocatalyst for the synthesis and modification of different compounds. They are an accessible and inexpensive enzyme for the preparation of reaction objects and have recently received attention. Laccase purification was performed from basidiomycete Lentinus strigosus (LS) in several stages: Stage 1. On ion-exchange chromatography on TEAE Servacell 23 (400 ml), two distinctly separated laccase activity peaks were observed, eluted from the carrier at 0.21 and 0.27 M NaCl. In order to reduce the loss of enzymes, all fractions with laccase activity were collected, concentrated, and desalted using an ultrafiltration cell (Amicon, United States) with a UM-10 membrane. Stage 2. The resulting preparation with laccase activity was applied to a Q-Sepharose column (60 ml). Two well-separated peaks with laccase activity were obtained during the elution: laccase I (0.12 M NaCl) and laccase II (0.2 M NaCl). Stage 3. In the course of further purification of both enzymes, carried out on anion-exchange carrier Resource Q (6 ml), a broken gradient was used: 0 - 10%, 10 - 20%, and 20 - 100% with 1M NaCl. Stage 4. Both laccase I and laccase II, obtained after Resource Q, were desalted, concentrated to 1 ml each, and applied to a Superdex 75 gel filtration column. As a result, two laccases were obtained in a homogeneous form.

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“…During our recent studies examining bioactive metabolites from endophytes, 15–18 Alternaria alternata ZHJG5, a Cercis chinensis ‐associated fungus, showed strong antibacterial effects against many phytopathogens. A study of the crude extract of A. alternata ZHJG5 led to the discovery of five new polyketide derivatives, isotalaroflavone ( 2 ), (+/−)‐5′‐dehydroxytalaroflavone ( 3a/3b ), (+)‐talaroflavone ( 4b ) and bialternacin G ( 7 ), along with five known compounds.…”

Section: Introductionmentioning

confidence: 99%

Novel metabolites from the Cercis chinensis derived endophytic fungus Alternaria alternataZHJG5 and their antibacterial activities

Zhao

Wang

Tian

et al. 2021

Pest Management Science

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BACKGROUND Phytopathogenic bacteria, such as Xanthomonas oryzae pv. oryzae (Xoo) and Ralstonia solanacearum (Rs), seriously threaten crop production and are the cause of enormous yield losses. Endophytic fungi are abundant sources of bioactive metabolites that may be potential candidates in the development of new agrochemicals. This work emphasizes the discovery of bioactive polyketides from endophytic Alternaria alternata ZHJG5 and reports their structural elucidation and antibacterial activities in detail. RESULTS Five novel polyketide derivatives, isotalaroflavone (2), (+/−)‐5′‐dehydroxytalaroflavone (3a/3b), (+)‐talaroflavone (4b), and bialternacin G (7), along with five known compounds (1, 4a, 5, 6, and 8), were obtained from the Cercis chinensis‐derived fungus A. alternata ZHJG5. The compounds' structures were characterized using spectroscopic methods and X‐ray diffraction. Chiral high‐performance liquid chromatography was used to separate the racemates 3 and 4, whose absolute configurations were unambiguously confirmed by comparing their experimental and calculated electron circular dichroism data. All isolated compounds were tested for antibacterial activity against the phytopathogenic bacteria Xoo, Xanthomonas oryzae pv. oryzicola (Xoc) and Rs. Compounds 1, 2 and 8 showed pronounced antibacterial activity against all tested bacteria, with minimal inhibitory concentrations ranging from 0.5 to 64 μg/ml. In addition, compound 1 showed a potent protective effect against rice bacterial leaf blight caused by Xoo with a protective efficacy of 75.1% at a concentration of 200 μg/ml. CONCLUSION These findings highlight the practical potential of antibacterial compounds as candidates for the discovery of novel bactericides. © 2021 Society of Chemical Industry

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Discovery of Natural FabH Inhibitors Using an Immobilized Enzyme Column and Their Antibacterial Activity against Xanthomonas oryzae pv. oryzae

Cited by 18 publications

References 35 publications

Fungal Endophytes: A Potential Source of Antibacterial Compounds

Fungal Endophytes: A Potential Source of Antibacterial Compounds

Testing for the ability to modify antibiotics of Panus tigrinus 8/18 Lentinus strigosus 1566 laccase

Novel metabolites from the Cercis chinensis derived endophytic fungus Alternaria alternataZHJG5 and their antibacterial activities

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