Discovery of Nanos1 and Nanos2/3 as Germ Cell Markers During Scallop Gonadal Development

Liu, Liangjie; Liu, Tian; Wu, Shihao; Li, Yajuan; He, Wei; Zhang, Lijing; Shu, Ya; Yang, Yaxin; Xing, Qiang; Wang, Shi; Zhang, Lingling

doi:10.1007/s10126-022-10124-0

Cited by 8 publications

(7 citation statements)

References 33 publications

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“…In the trochophore larva, hybridization signals specifically appeared in three cells without any signals elsewhere (Figure 7). The distribution of Ml-prdm1 transcripts around the gastrula stage was remarkably similar to some of the putative PGC markers, especially nanos, in other bivalves [8,9]. In addition, Ml-prdm1-positive cells exhibited slow cell cycles from the blastula to the trochophore larva, and this was also consistent with observations in other mollusks in which PGCs were mitotically quiescent for a period [73,74].…”

Section: Discussionsupporting

confidence: 86%

“…Usually, a subset of cells labeled by marker genes, such as vasa and nanos , are identified as putative PGCs or their precursors in mollusks. Although the expression patterns of these markers throughout embryonic development are not completely the same, the bivalve blastula and gastrula shared a similar subcellular localization of markers, which were restricted to two cells or cell clumps [ 7 , 8 , 9 , 43 ]. In the present study, Ml-prdm1 exhibited a restricted expression in the embryos and larvae of M. lateralis .…”

Section: Discussionmentioning

confidence: 99%

“…These two mechanisms are not mutually exclusive and are inevitably used simultaneously at a certain stage of germ cell development [ 6 ]. So far, the PGC specification modes are different among different species, and both theories are reported in mollusks [ 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, understanding the molecular mechanisms of germ cells will facilitate the development of propagation control techniques and promote the aquaculture industry [ 40 , 41 , 42 ]. However, studies on the origin and development of PGCs in mollusks are scarce, and currently only three genes, vasa , nanos , and piwi , are used in related studies [ 7 , 8 , 9 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification and Expressional Analysis of Putative PRDI-BF1 and RIZ Homology Domain-Containing Transcription Factors in Mulinia lateralis

Zhao

Guo

et al. 2023

Biology

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Mollusca represents one of the ancient bilaterian groups with high morphological diversity, while the formation mechanisms of the precursors of all germ cells, primordial germ cells (PGCs), have not yet been clarified in mollusks. PRDI-BF1 and RIZ homology domain-containing proteins (PRDMs) are a group of transcriptional repressors, and PRDM1 (also known as BLIMP1) and PRDM14 have been reported to be essential for the formation of PGCs. In the present study, we performed a genome-wide retrieval in Mulinia lateralis and identified 11 putative PRDMs, all of which possessed an N-terminal PR domain. Expressional profiles revealed that all these prdm genes showed specifically high expression levels in the given stages, implying that all PRDMs played important roles during early development stages. Specifically, Ml-prdm1 was highly expressed at the gastrula stage, the key period when PGCs arise, and was specifically localized in the cytoplasm of two or three cells of blastula, gastrula, or trochophore larvae, matching the typical characteristics of PGCs. These results suggested that Ml-prdm1-positive cells may be PGCs and that Ml-prdm1 could be a candidate marker for tracing the formation of PGCs in M. lateralis. In addition, the expression profiles of Ml-prdm14 hinted that it may not be associated with PGCs of M. lateralis. The present study provides insights into the evolution of the PRDM family in mollusks and offers a better understanding of the formation of PGCs in mollusks.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Expressional Analysis of Putative PRDI-BF1 and RIZ Homology Domain-Containing Transcription Factors in Mulinia lateralis

Zhao

Guo

et al. 2023

Biology

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“…Nr4a1 may affect the ability of ovarian theca cells to produce androgens by regulating the transcription of the steroidogenic enzymes StAR, Cyp11a1, Cyp17 and HSD3B2 [ 41 ]. The Hsd17b gene comprises two genes, Hsd17b5 and Hsd17b6 , both of which encode key enzymes that facilitate the conversion of androstenedione to testosterone [ 42 ]. However, we did not find differential expression of Cyp17 , which we speculate may be due to the fact that the samples collected were at an early stage of development.…”

Section: Discussionmentioning

confidence: 99%

Screening of temperature-responsive signalling molecules during sex differentiation in Asian yellow pond turtle (Mauremys mutica)

Liu,

Xu,

Peng

et al. 2024

BMC Genomics

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Background The Asian yellow pond turtle (Mauremys mutica) is an important commercial freshwater aquaculture species in China. This species is a highly sexually dimorphic species, with males growing at a faster rate than females and exhibits temperature-dependent sex determination (TSD), in which the incubation temperature during embryonic development determines the sexual fate. However, the mechanisms of the sex determination or sex differentiation in the Asian yellow pond turtle are remain a mystery. Results Temperature-specific gonadal transcriptomics of the Asian yellow pond turtle were performed during the thermosensitive period (stage 15) using RNA-seq technology to identify candidate genes that initiate gonadal differentiation. We uncovered candidates that were the first to respond to temperature. These candidates were sexually dimorphic in expression, reflecting differences in gonadal (Cirbp, Runx1) and germline differentiation (Vasa, Nanos1, Piwil2), gametogenesis (Hmgb3, Zar1, Ovoinhibitor-like, Kif4), steroid hormone biosynthesis (Hsd17b5, Hsd17b6), heat shock (Dnajb6, Hsp90b1, Hsp90aa1) and transient receptor potential channel genes (Trpm1, Trpm4, Trpm6, Trpv1). Conclusions Our work will provide important genetic information to elucidate the mechanisms of sex control in the Asian yellow pond turtles, and will contribute important genetic resources for further studies of temperature-dependent sex determination in turtles.

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Integrated transcriptome and SNP analysis reveals sex-related genetic insights in the black-spotted frog (Pelophylax nigromaculatus)

Zhang,

Wan,

et al. 2024

Aquacult Int

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Discovery of Nanos1 and Nanos2/3 as Germ Cell Markers During Scallop Gonadal Development

Cited by 8 publications

References 33 publications

Identification and Expressional Analysis of Putative PRDI-BF1 and RIZ Homology Domain-Containing Transcription Factors in Mulinia lateralis

Identification and Expressional Analysis of Putative PRDI-BF1 and RIZ Homology Domain-Containing Transcription Factors in Mulinia lateralis

Screening of temperature-responsive signalling molecules during sex differentiation in Asian yellow pond turtle (Mauremys mutica)

Integrated transcriptome and SNP analysis reveals sex-related genetic insights in the black-spotted frog (Pelophylax nigromaculatus)

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